姜玮，男，1962年生人，1984年7月毕业于山东大学化学系，获理学学士学位并留校任教，分别于1992年和2003年在山东大学获理学硕士、博士学位。现任山东大学化学与化工学院教授，博士生导师，院学术委员会委员，山东大学基础学部学术委员会委员，山东省化学化工学会常务理事，中国化学会会员，中国化学会有机分析专业委员会委员，山东省环境学会理事。学习及工作经历：1984.07 － 1991.12 山东大学化学系，助教1991.12 － 1997.09 山东大学化学与化工学院，讲师1997.09 － 2002.09 山东大学化学与化工学院，副教授2002.09 － 现在 山东大学化学与化工学院，教授2006.06 － 现在 山东大学化学与化工学院，教授，博士生导师1999.11 － 2003.12 山东大学化学与化工学院，副院长2004.01 － 2007.12 山东大学研究生院，副院长2004.06 － 2007.12 兼任山东大学威海分校研究生处，处长2007.12 － 2013.01 山东大学化学与化工学院，党委书记兼副院长2013.01 － 2018.04 山东大学化学与化工学院，党委书记主讲课程：分析化学前沿，分析化学，无机及分析化学，分析化学实验，仪器分析实验研究领域和兴趣：研究领域为生物分析化学，研究工作集中在：1、DNA纳米材料的设计、制备、表征与功能化；2、多模态、多维度荧光显微成像技术与单分子检测、单细胞分析；3、新型荧光信号放大策略与生物分子超灵敏检测技术；4、新型纳米荧光生物传感器的设计、构建与生物医学应用。作为项目负责人承担国家自然科学基金面上项目5项，山东省自然科学基金重点项目2项。在Analytical Chemistry，Chemical Science, Biomaterials，Chemical Communications，Biosensor and Bioelectronics，ACS Applied Materials & Interfaces，Bioconjugate Chemistry等国际知名学术杂志发表SCI收录研究论文100余篇。主要论著：103. A bipedal-unequivalent three-dimensional DNA walker and its biosensing application Sensors and Actuators: B. Chemical 327 (2021) 128942102.A bimolecular i-motif mediated FRET strategy for imaging protein homodimerization on a living tumor cell surfaceChemical Communications, 2020, 56, 13405-13408101.An interparticle relatively motional DNA walker and its sensing applicationChemical Science, 2020, 11, 7415-7423100.A track-regenerated DNA walker: Construction and its derived sensing applicationSensors and Actuators B-Chemical, 2020, 314, 12805399.Multifunctional molecular beacons-modified gold nanoparticles as a nanocarrier for synergistic inhibition and in situ imaging of drug-resistant-related mRNAs in living cells.ACS Appl. Mater. Interfaces, 2019, 11, 35548-3555598.A DNA walker powered by endogenous enzymes for imaging uracil-DNA glycosylase activity in living cellsChemical Communications, 2019, 55, 6026-602997.pH-responsive ZnO nanoprobe mediated DNAzyme signal amplification strategy for sensitive detection and live cell imaging of multiple microRNAsSensors and Actuators B-Chemical, 2019, 293, 93-9996.MnO2 nanosheet-mediated ratiometric fluorescence biosensor for microRNA detection and imaging in living cellsAnalytica Chimica Acta, 2019, 1063, 152-15895.Multiple sealed primers-mediated rolling circle amplification strategy for sensitive and specific detection of DNA methyltransferase activityTalanta, 2019, 194, 282-288.94.T7 exonuclease-assisted and target-triggered cascade dual recycling signal amplification strategy for the sensitive and specific detection of adenosineTalanta, 2019, 197, 234-23893.Endogenous stimuli-responsive nucleus-targeted nanocarrier for intracellular mRNA imaging and drug deliveryACS Appl. Mater. Interfaces 2018, 10, 39524-3953192.Binding-induced nicking site reconstruction strategy for quantitative detection of membrane protein on living cellTalanta, 2018, 189, 383-38891.An enzyme-free and label-free fluorescence biosensor for microRNA detection based on cascade amplification of DNAzyme-powered three-dimensional DNA walker and hybridization chain reactionSensors and Actuators B-Chemical, 2018, 268, 287-29290.Protein binding protection in combination with DNA masking for sensitive and reliable transcription factor detectionTalanta, 2018, 186, 293-29889.A smart DNA tweezer for detection of human telomerase activityAnalytical Chemistry, 2018, 90, 3521-353088.An integrated and restructive probe mediated strand displacement amplification strategy for sensitive and specific DNA methyltransferase activity detectionSensors and Actuators B-Chemical, 2018, 266, 124-13087.Multifunctional aptamer probe mediated cascade amplification for label-free detection of adenosineSensors and Actuators B-Chemical, 2018, 260, 581-58686.A dual-targeting DNA tetrahedron nanocarrier for breast cancer cell imaging and drug deliveryTalanta, 2018, 179, 356-36385.Target binding protection mediated rolling circle amplification for sensitive detection of transcription factorsTalanta, 2018, 179, 331-33684.Self-primer and self-template recycle rolling circle amplification strategy for sensitive detection of uracil-DNA glycosylase activity Analytica Chimica Acta, 2018, 1001, 119-12483.Convertible DNA ends-based silver nanoprobes for colorimetric detection human telomerase activityTalanta, 2018, 178, 458-46382.Integrating DNA structure switch with branched hairpins for the detection of uracil-DNA glycosylase activity and inhibitor screeningTalanta, 2018, 179, 51-5681.Sensitive and selective detection of the p53 gene based on a triple-helix magnetic probe coupled to a fluorescent liposome hybridization assembly via rolling circle amplificationAnalyst, 2017, 142, 3598-360480.Ultrasensitive and accurate assay of human methyltransferase activity at the single-cell level based on a single integrated magnetic microprobeACS Appl. Mater. Interfaces, 2017, 9, 29554-2956179.Liposome-encoded magnetic beads initiated by padlock exponential rolling circle amplification for portable and accurate quantification of microRNAsChemical Communications, 2017, 53, 10772-1077578.Uracil removal-inhibited ligase reaction in combination with catalytic hairpin assembly for sensitive and specific detection of uracil-DNA glycosylase activityAnalyst, 2017, 142, 4655-466077.DNA nanostructure-based drug delivery nanosystems in cancer therapyInternational Journal of Pharmaceutics, 2017, 533, 169-17876.A catalytic assembled enzyme-free three-dimensional DNA walker and its sensing applicationChemical Communications, 2017, 53, 5527-553075.Terminal protection-mediated autocatalytic cascade amplification coupled with graphene oxide fluorescence switch for sensitive and rapid detection of folate receptor Talanta, 2017, 174, 684-68874.A target triggered proximity combination-based fluorescence sensing strategy for adenosine detectionAnalyst, 2017, 142, 2247-2252 73.A loop-mediated cascade amplification strategy for highly sensitive detection of DNA methyltransferase activitySensors and Actuators B-Chemical, 2017, 244, 599-60572.Sensitive detection of T4 polynucleotide kinase activity based on multifunctional magnetic probes and polymerization nicking reactions mediated hyperbranched rolling circle amplificationBiosensors and Bioelectronics, 2017, 91, 631-63671.Label-free molecular beacons-based cascade amplification DNA machine for sensitive detection of telomerase activityTalanta, 2017, 167, 645-65070.Toehold-mediated strand displacement reaction-dependent fluorescent strategy for sensitive detection of uracil-DNA glycosylase activityBiosensors and Bioelectronics, 2017, 89, 984-98869.Colocalization recognition-activated cascade signal amplification strategy for ultrasensitive detection of transcription factorsBiosensors and Bioelectronics, 2017, 89, 978-98368.A bicyclo-hairpin probe mediated strand displacement amplification strategy for label-free and sensitive detection of bleomycinSensors and Actuators B, 2017, 238, 318-32467.Model-guided interface probe arrangement for sensitive protein detectionAnalytical Chemistry, 2016, 88, 9885-988966.Application of an ultrahigh-performance liquid chromatography coupled to quadrupole-Orbitrap high-resolution mass spectrometry for the rapid screening, identification and quantification of illegal adulterated glucocorticoids in herbal medicinesJournal of Chromatography B, 2016, 1038, 34-4265.Target-controlled gating liposome "off–on"cascade amplification for sensitive and accurate detection of phospholipase D in breast cancer cells with a low-background signalChemical Communications, 2016, 52, 10660-1066364.Binding Induced Colocalization Activated Hybridization Chain Reaction on the Surface of Magnetic Nanobead for Sensitive Detection of AdenosineBiosensors and Bioelectronics, 2016, 86, 966--97063.A split recognition mode combined with cascade signal amplification strategy for highly specific, sensitive detection of microRNABiosensors and Bioelectronics, 2016, 86, 834-83962.T-Hg2+-T metallo-base pair-mediated dual amplification fluorescent strategy for the selective and sensitive detection of Hg2+RSC Advances, 2016, 6, 70984-7098961.Protein binding-protected DNA three-way junction-mediated rolling circle amplification for sensitive and specific detection of transcription factorsRSC Advances, 2016, 6, 68846-6885160.Modular nuclease-responsive DNA three-way junctions-based dynamic assembly of DNA device and its sensing applicationAnalytical Chemistry, 2016, 88, 3817-382559.A unique dual recognition hairpin probe mediated fluorescent amplification method for sensitive detection of uracil-DNA glycosylase and endonuclease IV activitiesAnalyst，2016, 141, 1789-179558.Au Nanoparticles Fluorescence Switch-mediated Target Recycling Amplification Strategy for Sensitive Nucleic Acid DetectionRSC Advances, 2016, 6, 10650-1065457.Cascade Signal Amplification Based on Copper Nanoparticle-Reported Rolling Circle Amplification for Ultrasensitive Electrochemical Detection of the Prostate Cancer BiomarkerACS Appl. Mater. Interfaces 2016, 8, 2573−258156.Multiplexed detection of cytokines based on dual bar-code strategy and single-molecule countingAnalytical Chemistry, 2016, 88, 1578-158455.Target-protected dumbbell molecular probe mediated cascade rolling circle amplification strategy for the sensitive assay of DNA methyltransferase activityChemical Communications, 2016, 52, 2517-252054.A dual amplification fluorescent strategy for sensitive detection of DNA methyltransferase activity based on strand displacement amplification and DNAzyme amplificationBiosensors and Bioelectronics, 2016, 77, 650-65553.Label-free and enzyme-free detection of transcription factors with graphene oxide fluorescence switch-based multifunctional G-quadruplex-hairpin probeBiosensors and Bioelectronics，2016, 75, 155-16052.Label-free detection of nicotinamide adenine dinucleotide based on ligation-triggered exonuclease III-assisted signal amplificationRSC Advances, 2015, 5, 86625-8663051.Label-free Molecular Beacons-Based Quadratic Isothermal exponential Amplification: A simple and Sensitive One-Pot Method to Detection DNA Methyltransferase ActivityChemical Communications, 2015, 51, 13538-1354150.Hairpin assembly circuit-based fluorescence cooperative amplification strategy for enzyme-free and label-free detection of small moleculeTalanta, 2015, 143: 101-10649.FokI cleavage–inhibition strategy for the specific and accurate detection of transcription factors Talanta, 2015,144, 44-5048.Label-free and dual-amplified detection of protein via small molecule-ligand linked DNA and a cooperative DNA machineBiosensors and Bioelectronics, 2015, 72, 107-11347.Highly selective and sensitive detection of miRNA based on toehold-mediated strand displacement reaction and DNA tetrahedron substrate Biosensors and Bioelectronics, 2015, 71, 401-40646.Label-free fluorescence dual-amplified detection of adenosine based on exonucleaseIII-assisted DNA cycling and hybridization chain reactionBiosensors and Bioelectronics, 2015, 70, 15-20 45.Label-free Nucleic Acid Detection Based on DNA Templated Silver Nanoclusters Fluorescent ProbeTalanta, 2015, 138, 163-16844.A DNA machine-based fluorescence amplification strategy for sensitive detection of uracil-DNA glycosylase activityBiosensors and Bioelectronics, 2015, 68, 654-65943.A Binding-induced Sutured Toehold Activation for Controllable DNA Strand Displacement ReactionsChemical Communications, 2015, 51, 2903-290642.Sensitive detection of transcription factors with Ag+-stabilized self-assembly triplex DNA molecular switch Chemical Communications, 2014, 50, 14987-1499041.Visualizing the endocytosis of phenylephrine in living cells by quantum dot-based trackingBiomaterials, 2014, 35, 7042-704940.Quantitative detection of tumor necrosis factor-α by single molecule counting based on hybridization chain reactionBiosensors and Bioelectronics, 2014, 60, 180-18439.Toehold-mediated Strand Displacement Reaction Triggered Isothermal DNA Amplification for Highly Sensitive and Selective Fluorescent Detection of Single-base MutationBiosensors and Bioelectronics, 2014, 59, 276-28138.Cascade Signal Amplification Strategy for Sensitive and Label-free DNA Detection Based on Exo III-catalyzed Recycling Coupled with Rolling Circle AmplificationAnalyst, 2014, 139, 2884-288937.Aptamer-Based Exonuclease Protection and Enzymatic Recycling Cleavage Amplification Homogeneous Assay for Highly sensitive Detection of thrombinAnalyst, 2014, 139, 3167-317336.Quantitative Single-molecule Detection of Protein Based on DNA Tetrahedron Fluorescent Nanolabels Talanta, 2014, 125, 393-39935.An ultrasensitive fluorescence assay for protein detection by hybridization chain reaction-based DNA nanotags Biosensors and Bioelectronics, 2014, 51, 421-42534.Surface-enhanced Raman spectroscopy detection of polybrominated diphenylethers using a portable Raman spectrometerTalanta, 2013, 116, 14-1733.Cysteamine-Modified Silver Nanoparticle Aggregates for Quantitative SERS Sensing of Pentachlorophenol with a Portable Raman Spectrometer ACS Appl. Mater. Interfaces, 2013, 5 (15), 6902-690832.Cysteine-Modulated Colorimetric Sensing of Extracellular Mg2+ in Rat Brain Based on the Strong Chelation Interaction between Dithiothreitol and Mg2+Analyst, 2013,138, 3046-3052 31.A novel label-free cascade amplification strategy based on dumbbell probe-mediated rolling circle amplification-responsive G-quadruplex formation for highly sensitive and selective detection of NAD+ or ATPChemical Communications, 2013, 49, 2640-264230.A Novel Enzyme-free and Label-free Fluorescence Aptasensor for Amplified Detection of AdenosineBiosensors and Bioelectronics, 2013, 44, 52-5629.Selective and Sensitive Mercuric (II) Ion Detection Based on Quantum Dots and Nicking Endonuclease Assisted Signal AmplificationBiosensors and Bioelectronics, 2013, 43, 84-8728.Electrospun porous CuO-Ag nanofibers for quantitative sensitive SERS detectionCrystEngComm, 2013, 15, 1339-134427.Visualization the Intracellular Location and Stability of DNA origami with a Label-free Fluorescent ProbeChemical Communications, 2012, 48, 11301-1130326.Quantitative Detection of Single DNA Molecules on DNA Tetrahedron Decorated SubstratesChemical Communications, 2012, 48, 9661-966325.In situ fabrication of single-crystalline porous ZnO nanoplates on zinc foil to support silver nanoparticles as a stable SERS substrateCrystEngComm, 2012, 14, 6023-6028 24.UV Photolysis-induced Generation of Graphene/M (M=Ag/Au) Hybrid NanomaterialsMaterials Letters, 2012, 84, 120-12323.Silver nanoparticle aggregates on copper foil for reliable quantitative SERS analtsis of polycyclic aromatic hydrocarbons with a portable Raman spectrometerAnalyst, 2012, 137, 3995-400022.Micrococcal Nuclease Detection Based on Peptide-Bridged Energy Transfer between Quantum Dots and Dye-Labeled DNATalanta, 2012, 97, 533-53821.Sensitive Detection of Proteins Using Assembled Cascade Fluorescent DNA Nanotags Based on Rolling Circle AmplicationBioconjugate Chemistry, 2012, 23, 734-73920.Strong Interaction between Imidazolium-Based Polycationic Polymer and Ferricyanide: toward Redox Potential Regulation for Selective In Vivo Electrochemical MeasurementsAnalytical Chemistry, 2012, 84, 1900-190619.A versatile platform for highly sensitive detection of protein: DNA enriching magnetic nanoparticles based rolling circle amplication immunoassayChemical Communications, 2012, 33, 3930-3932. Inside cover paper18.Synthesis of a quinazoline derivative: A new a 1-adrenoceptors ligand for conjugation to quantum dots to study a 1-adrenoceptors in living cellsBioorganic & Medicinal Chemistry Letters, 2011, 21, 5905-590917.Label free DNA detection based on gold nanoparticles quenching fluorescence of Rhodamine BTalanta, 2011, 85, 725-72916.A Sensitive Enzymatic Method for Paraoxon Detection based on Enzyme Inhibition and Fluorescence QuenchingTalanta, 2011, 84, 400-405 15.Quantitative Detection of Single Molecules Using Enhancement of Dye/DNA Conjugate-Labeled NanoparticlesBioconjugate Chemistry, 2010, 21, 1987-199314.Solid phase single-molecule counting of antibody binding to supported protein layers surface with low nonspecific adsorptionTalanta, 2010, 82, 1003-100913.Fluorescence single-molecule counting assays for protein quantification using epi-fluorescence microscopy with quantum dots labelingAnalytical Chimica Acta, 2010, 662, 170-17612.DNA Quantification Based on FRET Realized by Combination with Surfactant CPBTalanta, 2010, 81, 597-60111.The co-luminescence effect of a europium(III)-lanthanum(III)-gatifloxacin-sodium dodecylbenzene sulfonate system and its application for the determination of trace amount of europium(III)Journal of Luminescence, 2010, 130, 591-59710.Quantitative detection of antibody based on single-molecule counting by total internal reflection fluorescence microscopy with quantum dot labelingAnalytica Chimica Acta, 2009, 634, 83-8809.Rapid Determination of Minocycline in Pharmaceutical Formulations and Human Urine/Serum Samples Based on the Minocycline-Europium-Sodium Dodecylbenzene Sulfonate Luminescence SystemAnalytical Letters, 2009, 42, 228-242 08.Luminescence enhancement effect for the determination of balofloxacin with balofloxacin–europium (III)–sodium dodecylbenzene sulfonate systemJournal of Luminescence, 2009, 129, 90-9407.Micelle-enhanced and terbium-sensitized spectrofluorimetric determination of gatifloxacin and its interaction mechanismSpectrochimica Acta Part A, 2009, 72, 766-77106.Rapid determination of gatifloxacin in biological samples and pharmaceutical products using europium-sensitized fluorescence spectrophotometryLuminescence, 2008, 23, 7-1305.Quantification of protein based on single-molecule counting by total internal reflection fluorescence microscopy with adsorption equilibriumAnalytica Chimica Acta, 2007, 590, 104-10904.Fluorimetric Determination of Europium Based on the Co-fluorescence System Eu-Y-Sparfloxacin-SDSMikrochimica Acta, 2004, 144, 29-34 03.Determination of trace europium based on a new fluorescence system of europium-sparfloxacin-1,10-phenanthroline-sodium dodecyl sulfateAnalytical Bioanalytical Chemstry, 2003, 377(4), 681-684 02.Spectrofluorimetric determination of trace amounts terbium with 4-chlorsalicylic acid, EDTA, and cetyltrimethylammonium bromideAnalytical Sciences, 2003, 19, 923-92501.Study of the absorption spectra of the 4f electron transitions of the praseodymium complex with ciprofloxacin and its analytical applicationAnalyst, 2001, 126, 1440-1443科研项目：01. 国家基金面上项目：内源性刺激响应的复合DNA纳米探针的构建与活细胞内功能生物分子的原位成像及调控研究（No.22074081），2021.01-2024.12，主持02. 国家基金面上项目：基于功能化石墨烯的循环肿瘤细胞分选与活细胞原位荧光成像研究（No.21675100），2017.01-2020.12，主持03. 国家基金面上项目：新型细胞表面膜蛋白适体荧光探针的构建与活细胞原位分析研究（No.21675101），2017.01-2020.12，主持04. 国家基金面上项目：基于DNA催化自组装的模块化荧光生物传感技术及其生物医学应用研究 (No.21475077)，2015.01-2018.12，主持05. 国家基金面上项目：基于结构DNA纳米材料的生物单分子荧光成像技术研究 (No.21175081)，2012.01-2015.12，主持06. 国家基金面上项目：全内反射荧光显微术检测单个生物大分子 (No.20775043)，2008.01-2010.12，主持07. 山东省基金重点项目：量子点结合荧光共振能量转移技术检测生物大分子 (Z2008B05)，2009.01-2011.12，主持08. 山东省基金重点项目：单分子计数及细胞内单分子运动轨迹的观测 (Z2004B03 )，2005.01-2007.12，主持