王贞
副教授
所属院部： 齐鲁医学院
访问次数：次
基本信息
  • 教师拼音名称：
    wangzhen
  • 入职时间：
    2003-07-16
  • 所在单位：
    基础医学院
  • 学历：
    博士研究生毕业
  • 性别：
    女
  • 联系方式：
    wangzhen@sdu.edu.cn
  • 学位：
    博士
  • 在职信息：
    在职
  • 毕业院校：
    山东大学
  • 博士生导师
  • 硕士生导师
教育经历
  • 2000-9 — 2003-6
    山东大学
    生理学
    硕士
  • 2006-9 — 2011-6
    山东大学
    医学
    博士
  • 1993-9 — 1998-6
    新乡医学院
    临床医学
    学士
工作经历
  • 2003-7-至今
    山东大学医学院
  • 2000-9 — 2003-6
    新乡医学院
  • 1998-9 — 2000-8
    驻马店地区卫生学校
研究领域


自2003年以来，在山东大学基础医学院从事神经发育和中枢神经系统损伤与再生修复的研究，并致力于H2S在中枢神经系统损伤中的作用和机制研究，研究成果处于国内外领先水平。近来从事的系列研究一：“H2S对缺氧缺血性脑损伤的影响及其机制”，通过建立缺氧缺血性脑损伤的动物和细胞模型，从多角度研究内源性和外源性H2S对缺氧缺血性脑损伤的保护作用，探讨其可能机制。近年来发表相关论文14篇，旨在揭示H2S治疗缺氧缺血性脑损伤患者的新靶点和潜在价值，为临床的H2S治疗应用提供理论依据。系列研究二：“H2S对蛛网膜下腔出血的影响及其机制”，通过建立蛛网膜下腔出血（SAH）的动物模型，从多角度研究内源性和外源性H2S对SAH的保护作用，探讨其可能机制。系列研究三：“间充质干细胞来源的细胞外囊泡对对缺氧缺血性脑损伤的影响及其机制”，通过建立缺氧缺血性脑损伤的动物和细胞模型，从多角度研究无细胞疗法（细胞外囊泡）对缺氧缺血性脑损伤的保护作用，探讨其可能机制。全面阐明H2S修饰的细胞外囊泡对缺氧缺血性脑损伤后的神经保护作用及机制，为研发H2S改良外泌体治疗缺氧缺血性脑损伤的策略提供重要支持。


科研成果
论文

1.  . 3D Gelatin Microsphere Scaffolds Promote Functional Recovery after Spinal Cord Hemisection in Rats.  Advanced Science,  2023. 

2.  李婷婷. L-Cysteine attenuates osteopontin-mediated neuroinflammation following hypoxia-ischemia insult in neonatal mice by inducing S-sulfhydration of Stat3.  中国药理学报（英文版）,  2022. 

3.  李婷婷. L-Cysteine Provides Neuroprotection of Hypoxia-Ischemia Injury in Neonatal Mice via a PI3K/Akt-Dependent Mechanism.  DRUG DESIGN DEVELOPMENT AND THERAPY,  15,  517, 2021. 

4.  李婷婷. H2S prevents peripheral immune cell invasion, increasing [Ca2+]i and excessive phagocytosis following hypoxia-ischemia injury in neonatal mice.  Biomed Pharmacother.,  135,  2021. 

5.  辛丹清. MSCs-extracellular vesicles attenuated neuroinflammation, synapse damage and microglial phagocytosis after hypoxia-ischemia injury by preventing osteopontin expression.  《Pharmacol Res.》,  164,  2021. 

6.  韩敏. Mesenchymal stem cell-derived extracellular vesicles promote microglial M2 polarization after subarachnoid hemorrhage in rats and involve the AMPK/NF-kappa B signaling pathway.  Biomed Pharmacother.,  133,  2021. 

7.  初锡丽. Hydrogen sulfide-modified extracellular vesicles from mesenchymal stem cells for treatment of hypoxic-ischemic brain injury.  Journal of Controlled Release,  328,  13, 2020. 

8.  辛丹清. Mesenchymal stromal cell-derived extracellular vesicles modulate microglia/macrophage polarization and protect the brain against hypoxia-ischemic injury in neonatal mice by targeting delivery of miR-21a-5p.  Acta Biomaterialia,  2020. 

9.  初锡丽. Hydrogen sulfide-modified extracellular vesicles from mesenchymal stem cells for treatment of hypoxic-ischemic brain injury. Journal of Controlled Release.  Journal of Controlled Release,  2020. 

10.  辛丹清. Mesenchymal stromal cell-derived extracellular vesicles modulate microglia/macrophage polarization and protect the brain against hypoxia-ischemic injury in neonatal mice by targeting delivery of miR-21a-5p.  Acta Biomaterialia,  113,  597, 2020. 

11.  柯鸿飞. Hydrogen-Rich Saline Regulates Microglial Phagocytosis and Restores Behavioral Deficits Following Hypoxia-Ischemia Injury in Neonatal Mice via the Akt Pathway.  DRUG DESIGN DEVELOPMENT AND THERAPY,  14,  3827, 2020. 

12.  柯鸿飞. Hydrogen-Rich Saline Regulates Microglial Phagocytosis and Restores Behavioral Deficits Following Hypoxia-Ischemia Injury in Neonatal Mice via the Akt Pathway.  DRUG DESIGN DEVELOPMENT AND THERAPY,  2020. 

13.  刘德祥. Purmorphamine Attenuates Neuro-Inflammation and Synaptic Impairments After Hypoxic-Ischemic Injury in Neonatal Mice via Shh Signaling..  FRONTIERS IN PHARMACOLOGY,  2020. 

14.  刘德祥. Purmorphamine Attenuates Neuro-Inflammation and Synaptic Impairments After Hypoxic-Ischemic Injury in Neonatal Mice via Shh Signaling.  FRONTIERS IN PHARMACOLOGY,  11,  2020. 

15.  熊叶. Neuroprotective Mechanism of L-cysteine After Subarachnoid Hemorrhage.  Neural Regeneration Research,  2020. 

16.  韩敏. Evaluation of H2S-producing enzymes in cerebrospinal fluid and its relationship with interleukin-6 and neurologic deficits in subarachnoid hemorrhage..  Biomedicine & Pharmacotherapy,  2020. 

17.  韩敏. Neuroprotective Effect of Mesenchymal Stromal Cell-Derived Extracellular Vesicles Against Cerebral Ischemia-Reperfusion-Induced Neural Functional Injury: A Pivotal Role for AMPK and JAK2/STAT3/NF-κB Signaling Pathway Modulation.  DRUG DESIGN DEVELOPMENT AND THERAPY,  2020. 

18.  韩敏. Neuroprotective Effect of Mesenchymal Stromal Cell-Derived Extracellular Vesicles Against Cerebral Ischemia-Reperfusion-Induced Neural Functional Injury: A Pivotal Role for AMPK and JAK2/STAT3/NF-kappa B Signaling Pathway Modulation.  DRUG DESIGN DEVELOPMENT AND THERAPY,  14,  2865, 2020. 

19.  熊叶. Neuroprotective mechanism of L-cysteine after subarachnoid hemorrhage.  Neural Regeneration Research,  15,  1920, 2020. 

20.  谢云凯. Resveratrol reduces brain injury after subarachnoid hemorrhage by inhibiting oxidative stress and endoplasmic reticulum stress.  Neural Regeneration Research,  2019. 

21.  韩敏. Evaluation of H2S-producing enzymes in cerebrospinal fluid and its relationship with interleukin-6 and neurologic deficits in subarachnoid hemorrhage.  Biomed Pharmacother.,  123,  2020. 

22.  尹森. Neuroprotective Effects of the Sonic Hedgehog Signaling Pathway in Ischemic Injury through Promotion of Synaptic and Neuronal Health.  Neural Plasticity,  2020. 

23.  初锡丽 , 刘德祥 , 李婷婷 , 柯鸿飞 , 辛丹清 , 王书涵 , 曹颖 , 薛皓  and 王贞. Hydrogen sulfide-modified extracellular vesicles from mesenchymal stem cells for treatment of hypoxic-ischemic brain injury.  Journal of Controlled Release,  328,  13-27, 2020. 

24.  辛丹清 , 李婷婷 , 初锡丽 , 柯鸿飞 , 于卓雅 , 曹丽丽 , 柏雪梅 , 刘德祥  and 王贞. Mesenchymal stromal cell-derived extracellular vesicles modulate microglia/macrophage polarization and protect the brain against hypoxia-ischemic injury in neonatal mice by targeting delivery of miR-21a-5p.  Acta Biomaterialia,  597-613, 2020. 

25.  辛丹清 , 初锡丽 , 柏雪梅 , 马薇薇 , 袁洪涛 , 邱杰 , 刘长兴 , 李彤 , 周鑫 , 陈文强 , 刘德祥  and 王贞. L-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation.  Brain Behavior and Immunity,  222-234, 2018. 

26.  柳松 , 辛丹清 , 王凌霄 , 张甜甜 , 柏雪梅 , 李彤 , 谢云凯 , 薛皓 , 薄仕世 , 刘德祥  and 王贞. Therapeutic effects of L-Cysteine in newborn mice subjected to hypoxia-ischemia brain injury via the CBS/H2S system: role of oxidative stress and endoplasmic reticulum stress.  Redox Biology,  528-540, 2017. 

27.  辛丹清 , 李婷婷 , 初锡丽 , 柯鸿飞 , 刘德祥  and 王贞. MSCs-extracellular vesicles attenuated neuroinflammation, synapse damage and microglial phagocytosis after hypoxia-ischemia injury by preventing osteopontin expression.  Pharmacological Research,  105322, 2021. 

28.  初锡丽 , 曹丽丽 , 于卓雅 , 辛丹清 , 李婷婷 , 马薇薇 , 周鑫 , 陈文强 , 刘德祥  and 王贞. Hydrogen-rich saline promotes microglia M2 polarization and complement-mediated synapse loss to restore behavioral deficits following hypoxia-ischemic in neonatal mice via AMPK activation.  Journal of Neuroinflammation,  16,  104, 2019. 

29.  李彤 , 柳瀚森 , 薛皓 , 张金森 , 韩晓 , 杨绍峰 , 薄仕世 , 袁琳 , 邓林 , 李刚  and 王贞. Neuroprotective effects of hydrogen sulfide against early brain injury and secondary cognitive deficits following subarachnoid hemorrhage.  Brain Pathology,  27,  51-63, 2017. 

30.  袁琳 , 柳松 , 柏雪梅 , 高岩 , 刘广恒 , 王雪儿 , 刘德祥 , 李彤 , 郝爱军  and 王贞. Oxytocin inhibits lipopolysaccharide-induced inflammation in microglial cells and attenuates microglial activation in lipopolysaccharide-treated mice.  Journal of Neuroinflammation,  13,  77, 2016. 

31.  周鑫 , 初锡丽 , 辛丹清 , 李婷婷 , 柏雪梅 , 邱杰 , 袁洪涛 , 刘德祥 , 王大川  and 王贞. L-Cysteine-Derived H2S Promotes Microglia M2 Polarization via Activation of the AMPK Pathway in Hypoxia-Ischemic Neonatal Mice. Frontier in Molecular Neuroscience..  Frontier in Molecular Neuroscience,  58, 2019. 

32.  刘德祥 , 柏雪梅 , 马薇薇 , 辛丹清 , 初锡丽 , 袁洪涛 , 邱杰 , 柯鸿飞 , 尹森 , 陈文强  and 王贞. Purmorphamine Attenuates Neuro-Inflammation and Synaptic Impairments After Hypoxic-Ischemic Injury in Neonatal Mice via Shh Signaling.  Frontiers in Pharmacology,  204, 2020. 

33.  李婷婷 , 初锡丽 , 辛丹清 , 柯鸿飞 , 王书涵 , 刘德祥 , 陈文强  and 王贞. H2S prevents peripheral immune cell invasion, increasing [Ca2+]i and excessive phagocytosis following hypoxia-ischemic injury in neonatal mice.  Biomedicine & Pharmacotherapy,  111207, 2021. 

34.  韩敏 , 曹颖 , 郭晓凡 , 初锡丽 , 李婷婷 , 薛皓 , 辛丹清 , 袁琳 , 柯鸿飞 , 李刚  and 王贞. Mesenchymal stem cell-derived extracellular vesicles promote microglial M2 polarization after subarachnoid hemorrhage in rats and involve the AMPK/NF-κB signaling pathway.  Biomedicine & Pharmacotherapy,  111048, 2021. 

35.  李婷婷 , 李江冰 , 李彤 , 赵一静 , 柯鸿飞 , 王双连 , 刘德祥  and 王贞. L-Cysteine provides neuroprotection of hypoxia-ischemia injury in neonatal mice via a PI3K/Akt-dependent mechanism.  Drug Design Development and Therapy,  517-529, 2021. 

36.  尹森 , 柏雪梅 , 辛丹清 , 李婷婷 , 初锡丽 , 柯鸿飞 , 韩敏 , 陈文强 , 李新刚  and 王贞. Neuroprotective Effects of the Sonic Hedgehog Signaling Pathway in Ischemic Injury through Promotion of Synaptic and Neuronal Health.  Neural Plasticity,  8815195, 2020. 

37.  韩敏 , 刘德祥 , 邱杰 , 袁洪涛 , 胡泉 , 薛皓 , 李婷婷 , 马薇薇 , 张琼 , 李刚  and 王贞. Evaluation of H2S-producing enzymes in cerebrospinal fluid and its relationship with interleukin-6 and neurologic deficits in subarachnoid hemorrhage.  Biomedicine & Pharmacotherapy,  109722, 2020. 

38.  熊叶 , 辛丹清 , 胡泉 , 王凌霄 , 邱杰 , 袁洪涛 , 初锡丽 , 刘德祥 , 李刚  and 王贞. Neuroprotective Mechanism of L-cysteine After Subarachnoid Hemorrhage.  Neural Regeneration Research,  15,  1920-1930, 2020. 

39.  韩敏 , 曹颖 , 薛皓 , 初锡丽 , 李婷婷 , 辛丹清 , 袁琳 , 柯鸿飞 , 李刚  and 王贞. Neuroprotective Effect of Mesenchymal Stromal Cell-Derived Extracellular Vesicles Against Cerebral Ischemia-Reperfusion-Induced Neural Functional Injury: A Pivotal Role for AMPK and JAK2/STAT3/NF-κB Signaling Pathway Modulation.  Biomedicine & Pharmacotherapy,  111048, 2021. 

40.  柯鸿飞 , 刘德祥 , 李婷婷 , 初锡丽 , 辛丹清 , 韩敏 , 王双连  and 王贞. Hydrogen-Rich Saline Regulates Microglial Phagocytosis and Restores Behavioral Deficits Following Hypoxia-Ischemia Injury in Neonatal Mice via the Akt Pathway.  Drug Design Development and Therapy,  3827-3839, 2020. 

41.  杨春雷 , 李彤 , 薛皓 , 王凌霄 , 邓林 , 谢云凯 , 柏雪梅 , 辛丹清 , 袁洪涛 , 邱杰 , 王贞  and 李刚. Inhibition of Necroptosis Rescues SAH-Induced Synaptic Impairments in Hippocampus via CREB-BDNF Pathway.  Frontier in Neuroscience,  990, 2020. 

42.  李江冰 , 薛皓 , 李婷婷 , 初锡丽 , 辛丹清 , 熊叶 , 邱伟 , 高校 , 钱明宇 , 徐建业 , 王贞  and 李刚. Exosomes derived from mesenchymal stem cells attenuate the progression of atherosclerosis in ApoE-/- mice via miR-let7 mediated infiltration and polarization of M2 macrophage.  Biochemical and Biophysical Research Communications,  565-572, 2019. 

43.  谢云凯 , 周鑫 , 袁洪涛 , 邱杰 , 辛丹清 , 初锡丽 , 王大川  and 王贞. Resveratrol reduces brain injury after subarachnoid hemorrhage by inhibiting oxidative stress and endoplasmic reticulum stress.  Neural Regeneration Research,  14,  1734-1742, 2019. 

44.  李彤 , 王凌霄 , 胡泉 , 柳松 , 柏雪梅 , 谢云凯 , 张甜甜 , 薄仕世 , 高向前 , 吴淑华 , 李刚  and 王贞. Neuroprotective Roles of L-Cysteine in Attenuating Early Brain Injury and Improving Synaptic Density via the CBS/H2S Pathway Following Subarachnoid Hemorrhage in Rats.  Frontiers in Neurology,  176, 2017. 

45.  葛丽 , 魏丽华 , 杜长青 , 宋国华 , 薛亚卓 , 石浩申 , 杨明 , 尹欣欣 , 李润庭 , 王雪儿  and 王贞. Hydrogen-rich saline attenuates spinal cord hemisection-induced testicular injury in rats.  Oncotarget,  8,  42314-42331, 2017. 

46.  胡泉 , 李彤 , 王凌霄 , 谢云凯 , 柳松 , 柏雪梅 , 张甜甜 , 薄仕世 , 辛丹清 , 薛皓 , 李刚  and 王贞. Neuroprotective Effects of a Smoothened Receptor Agonist against Early Brain Injury after Experimental Subarachnoid Hemorrhage in Rats.  Frontiers in Cellular Neuroscience,  306, 2017. 

47.  柏雪梅 , 柳松 , 袁琳 , 谢云凯 , 李彤 , 王凌霄 , 王雪儿 , 张甜甜 , 秦树存 , 宋国华 , 葛丽  and 王贞. Hydrogen-rich saline mediates neuroprotection through the regulation of endoplasmic reticulum stress and autophagy under hypoxia-ischemia neonatal brain injury in mice.  Brain Research,  410-7, 2016. 

48.  张群 , 柳松 , 李彤 , 袁琳 , 柳瀚森 , 王雪儿 , 王富武 , 王双连 , 郝爱军 , 刘德祥  and 王贞. Preconditioning of bone marrow mesenchymal stem cells with hydrogen sulfide improves their therapeutic potential.  Oncotarget,  7,  58089-58104, 2016. 

49.  张群 , 袁琳 , 张庆瑞 , 高岩 , 刘广恒 , 修萌 , 魏翔  and 王贞. Resveratrol attenuates hypoxia-induced neurotoxicity through inhibiting microglial activation.  International Immunopharmacology,  28,  578-587, 2015. 

50.  张群 , 袁琳 , 刘德祥 , 王建梅 , 王双连 , 张庆瑞 , 龚艳粉 , 刘宏达 , 郝爱军  and 王贞. Hydrogen sulfide attenuates hypoxia-induced neurotoxicity through inhibiting microglial activation.  Pharmacological Research,  32-44, 2014. 

51.  刘德祥 , 谢凯 , 杨旭东 , 顾建华 , 李葛 , 王雪儿  and 王贞. Resveratrol reverses the effects of chronic unpredictable mild stress on behavior, serum corticosterone levels and BDNF expression in rats.  Behavioural Brain Research,  9-16, 2014. 

52.  刘德祥 , 张庆瑞 , 顾建华 , 王雪儿 , 谢凯 , 仙秀英 , 王建梅 , 江虹  and 王贞. Resveratrol prevents impaired cognition induced by chronic unpredictable mild stress in rats.  Progress in Neuro-Psychopharmacology and Biological Psychiatry,  21-9, 2014. 

53.  王贞 , 张群 , 袁琳 , 王双连 , 刘立伟 , 杨旭东  and 郝爱军. The effects of curcumin on depressive-like behavior in mice after lipopolysaccharide administration.  Behavioural Brain Research,  282-90, 2014. 

54.  王贞 , 刘德祥 , 张群 , 王建梅 , 詹景敏 , 仙秀英 , 杜朝霞 , 王雪儿  and 郝爱军. Palmitic acid affects proliferation and differentiation of neural stem cells in vitro.  Journal of Neuroscience Research,  922,  574-86, 2014. 

55.  江虹 , 王贞 , 王一鹤 , 谢凯 , 张庆瑞 , 栾钦松 , 陈文强  and 刘德祥. Antidepressant-like effects of curcumin in chronic mild stress of rats: Involvement of its anti-inflammatory action.  Progress in Neuro-Psychopharmacology and Biological Psychiatry,  33-9, 2013. 

56.  王贞 , 刘德祥 , 詹景敏 , 谢凯 , 王雪儿 , 仙秀英 , 顾建华 , 陈文强  and 郝爱军. Melatonin improves short and long-term neurobehavioral deficits and attenuates hippocampal impairments after hypoxia in neonatal mice.  Pharmacological Research,  84-97, 2013. 

57.  王贞 , 刘德祥 , 王富武 , 张群 , 杜朝霞 , 詹景敏 , 袁秋华 , Ling Eng-Ang  and 郝爱军. L-cysteine promotes the proliferation and differentiation of neural stem cells via the CBS/H2S pathway.  Neuroscience,  106-117, 2013. 

58.  王贞 , 詹景敏 , 王雪儿 , 顾建华 , 谢凯 , 张庆瑞  and 刘德祥. Sodium hydrosulfide prevents hypoxia-induced behavioral impairment in neonatal mice.  Brain Research,  126-34, 2013. 

59.  王贞 , 顾建华 , 王雪儿 , 谢凯 , 栾钦松 , 万年青 , 张群 , 江虹  and 刘德祥. Antidepressant-like activity of resveratrol treatment in the forced swim test and tail suspension test in mice: the HPA axis, BDNF expression and phosphorylation of ERK.  Pharmacology Biochemistry and Behavior,  104-10, 2013. 

60.  王贞 , 刘德祥 , 王富武 , 刘尚明 , 赵世斗 , Ling Eng-Ang  and 郝爱军. Saturated fatty acids activate microglia via Toll-like receptor 4/nuclear factor κB signaling.  British journal of nutrition. 2012 January 28; 107(2):,  107,  229-41, 2012. 

61.  Cai Lei , 王贞 , Ji Ailing , Meyer Jason M  and van der Westhuyzen Deneys R. Scavenger receptor CD36 expression contributes to adipose tissue inflammation and cell death in diet-induced obesity.  PloS one,  7,  e36785, 2013. 

62.  王贞 , 刘德祥 , 王俊涛 , 刘尚明 , 高明  and 郝爱军. Cytoprotective effects of melatonin on astroglial cells subjected to palmitic acid treatment in vitro.  Journal of pineal research,  52,  253-64, 2011. 

63.  刘德祥 , 王贞 , 刘尚明 , 王富武 , 赵世斗  and 郝爱军. Anti-inflammatory effects of fluoxetine in lipopolysaccharide (LPS)-stimulated microglial cells.  Neuropharmacology,  61,  592-9, 2011. 

专利

1. 骨桥蛋白在缺氧缺血性脑损伤中的应用

2. MSCs-EVs在制备治疗HI诱导的神经炎症的产品中的应用

3. MSCs-EVs在制备治疗HI诱导的神经炎症的产品中的应用

4. 用于早期检测动脉瘤性蛛网膜下腔出血严重程度及预后评估的生物标志物及其应用

5. 一种复苏生物实验中暂时停跳心脏的装置及方法

6. 硫化氢修饰的间充质干细胞外囊泡作为miRNA递送载体在缺氧缺血性脑损伤中的应用

7. 硫化氢修饰的间充质干细胞外囊泡作为miRNA递送载体在缺氧缺血性脑损伤中的应用

8. 一种复苏生物实验中暂时停跳心脏的装置

版权所有   ©山东大学　地址：中国山东省济南市山大南路27号　邮编：250100　
查号台：（86）-0531-88395114
值班电话：（86）-0531-88364731　建设维护：山东大学信息化工作办公室