房俊强， 博士， 副教授，博士生导师

Email: fangjunqiang@sdu.edu.cn

研究方向：

1. 均一糖胺聚糖的模块化酶法合成策略研究

2. 复杂寡糖的化学酶法合成及其应用

3. 糖核苷酸合成途径研究及规模化制备 

主持或参加科研项目：

1.  国家重点研发计划“绿色生物制造”子课题（2021YFC2103100）“医用多糖生物合成共性机制及透明质酸高产关键技术”2021-2025 主持

2. 国家重点研发计划“合成生物学专项”子课题（2018YF091704）“人乳寡糖多酶合成关键技术及应用”2019-2024 主持

3. 国家自然科学基金面上项目（31770854）“均一糖胺聚糖的酶法合成及其在肿瘤进展中的作用机理研究” 2018-2021  主持

4. 国家自然科学基金青年科学基金项目（31100588）“一种原核糖核苷酸合成酶的分子改造研究及其在稀有糖核苷酸合成中的应用” 2012-2014 主持

5. 山东省重点研发计划公益类（2019GSF107035）“杂合型糖胺聚糖寡糖片段的酶法合成及构效关系研究”2019-2020 主持

6. 山东省优秀中青年科学家科研奖励基金（BS2010SW002）“化学-酶法合成UDP-GlcNAc衍生物” 2010-2013   主持

7. 山东省博士后创新项目（201002019）“分子改造大肠杆菌K12尿苷转移酶拓宽底物选择性的研究” 2010-2012 主持

8. 山东大学自主创新基金（2010 GN034） “化学酶法合成氨基糖核苷酸衍生物” 2010-2012 主持

9. 国家高技术研究发展计划（863计划）课题（2012AA021504），“糖工程关键技术与重大产品开发”项目之课题4“糖链修饰与糖衍生物的研制”，2012-2015 研究骨干

10. 国家重大新药创制专项课题（2012ZX09502001-005）“糖疫苗及相关技术体系的建立与研究” 2012 -2015   研究骨干

11. 教育部科学技术研究重大项目（313033）“工程化大肠杆菌表达药用糖蛋白技术研究” 2012 -2015   研究骨干

代表性研究成果：

期刊论文：

24.  Yinshuang Li, Yi Li, Yuxi Guo, Congcong Chen, Lin Yang, Qian Jiang, Peixue Ling, Shuaishuai Wang,*, Lei Li,*, Junqiang Fang*.2024. Enzymatic modular synthesis of asymmetrically branched human milk oligosaccharides. Carbohydrate Polymers. 333,121908.

23.   Lin Yang, Shuaishuai Wang, Changsheng Chen, Changcheng Liu, Xiaoqin Wang, Shuang Li, Yi Li,Peixue Ling,* Zhongfu Wang,* and Junqiang Fang*.2024. Programmable Enzymatic Toolbox for the Assembly of Fucosylated Chondroitin Derivatives. ACS Catal. 2024, 14, 670−677.

22.    Shuang Li, Shuaishuai Wang, Jingyao Qu, Jingjun Han, Lin Yang, Yi Li, Lei Li, Qingwen Jia, Congcong Chen, Peixue Ling and Junqiang Fang*. 2023. Programmable One Cycle-One Disaccharide Unit Modular Synthesis of Hyaluronan and Chondroitin Hybrid Glycans. Green Chemistry ,25, 2021–2027.

21.   Jie Zheng, Han Xu, Junqiang Fang *and Xing Zhang *.2022. Enzymatic and chemoenzymatic synthesis of human milk oligosaccharides and derivatives. Carbohydrate Polymers, 291, 119564. 

20.    Lin Yang, Xuan Xu, Shuang Li, Yi Li, Peixue Ling, Junqiang Fang *. 2021. Robust one-pot multi-enzyme polysaccharide remodeling strategy for the synthesis of uniform chondroitin fragments and derivatives. Carbohydrate Research, 509.108442. 

19.    Shuang Li, Shuaishuai Wang, Yaqian Wang, Jingyao Qu, Xian-wei Liu, Peng George Wang, and Junqiang Fang*. 2021. Gram-scale production of sugar nucleotides and their derivatives. Green Chemistry, 23, 2628-2633. 

18.    Yaqian Wang, Shuang Li, Xuan Xu, Yujie Tan, Xian-wei Liu, Junqiang Fang*. 2020. Chemoenzymatic synthesis of homogeneous chondroitin polymers and its derivatives. Carbohydrate Polymers, 232, 115822.

17.    Shuang Li, Shuaishuai Wang, Xuan Fu, Xian-wei Liu, Peng George Wang, Junqiang Fang*. 2017 Sequential One-pot Multienzyme Synthesis of Hyaluronan and its Derivative. Carbohydrate Polymers, 178, 221-227. 

16.    Hailiang Zhu, Zhigang Wu, Madhusudhan Reddy Gadic, Shuaishuai Wang, Yuxi Guo, Garrett Edmundsc, Wanyi Guan*, Junqiang Fang*. 2017 Cation exchange assisted binding-elution strategy for enzymatic synthesis of human milk oligosaccharides (HMOs). Bioorg Med Chem Lett. 27(18):4285-4287.

15.   Angie D. Calderon,  Jun Zhou,  Wanyi Guan,  Zhigang Wu,  Yuxi Guo,  Jing Bai,  Qing Li,  Peng George Wang,  Junqiang Fang*  and  Lei Li*.2017 An enzymatic strategy to asymmetrically branched N-glycans. Org. Biomol. Chem., 15(35):7258-7262. 

14.    Xuan Fu,  Wenjing Shang,  Shuaishuai Wang,  Yunpeng Liu,  Jingyao Qu,  Xi Chen, Peng George Wang*  and  Junqiang Fang*.2017.  A general strategy for the synthesis of homogeneous hyaluronan conjugates and their biological applications. Chem. Commun., 53, 3555-3558.

13.    Yunpeng Liu, Liuqing Wen, Lei Li, Madhusudhan Reddy Gadi, Wanyi Guan, Kenneth Huang, Zhongying Xiao, Mohui Wei, Cheng Ma, Qing Zhang, Hai Yu, Xi Chen, Peng George Wang, Junqiang Fang*. 2016. A General Chemoenzymatic Strategy for the Synthesis of Glycosphingolipids. European J Org Chem,4315-4320. 

12.    Liuqing Wen*, Kenneth Huang, Yuan Zheng, Junqiang Fang*, Shukkoor Muhammed Kondengaden, Peng George Wang*.2016 Two-step enzymatic synthesis of 6-deoxy-L-psicose. Tetrahedron Letters, 57: 3819–3822. 

11.   Liuqing Wen, Kenneth Huang, Mohui Wei, Jeffrey Meisner, Yunpeng Liu, Kristina Garner, Lanlan Zang, Xuan Wang, Xu Li, Junqiang Fang, Houcheng Zhang, Peng George Wang.2015. Facile Enzymatic Synthesis of Ketoses. Angewandte Chemie, 127(43): 12845–12849.

10.   Shuaishuai Wang†, Xuan Fu†, Yunpeng Liu, Xian-wei Liu, Lin Wang, Junqiang Fang*, and Peng George Wang. 2015. Probing the roles of Conserved Residues in Uridyltransferase domain of Escherichia coli K12 GlmU by Site-directed Mutagenesis. Carbohydrate Research, 413(2): 70-74. 

9.      Yafei Zhai, Donglei Han, Ying Pan, Shuaishuai Wang, Junqiang Fang, Peng Wang, Xian-wei Liu. 2015. Enhancing GDP-fucose production in recombinant Escherichia coli by metabolic pathway engineering. Enzyme and Microbial Technology, 69:38-45.

8.      Yuxi Guo, Junqiang Fang, Tiehai Li, Xu Li, Cheng Ma, Xuan Wang, Peng G. Wang. 2015. Comparing substrate specificity of two UDP-sugar pyrophosphorylases and efficient one-pot enzymatic synthesis of UDP-GlcA and UDP-GalA. Carbohydrate Research, 411:1-5.

7.      Li, Tiehai; Wen, Liuqing; Williams, Adriel; Wu, Baolin; Li, Lei; Qu, Jingyao; Meisner, Jeffrey; Xiao, Zhongying; Fang, Junqiang; Wang, Peng George. 2014. Chemoenzymatic synthesis of ADP-d-glycero-β-d-manno-heptose and study of the substrate specificity of HldE. Bioorganic & Medicinal Chemistry, 22(3): 1139-1147

6.      Junqiang Fang, Mengyang Xue, Guofeng Gu, Xian-wei Liu*, Peng George Wang. 2013. A chemoenzymatic route to synthesize unnatural sugar nucleotides using a novel N-acetylglucosamine-1-phosphate pyrophosphorylase from Camphylobacter jejuni NCTC 11168. Bioorganic & Medicinal Chemistry Letters, 23(15): 4303-4307. 

5.      Mengyang Xue, Wanyi Guan, Yang Zou, Junqiang Fang*, Xian-wei Liu*, Peng George Wang, Fengshan Wang. 2012. Investigation of the nucleotide triphosphate substrate specificity of Homo sapiens UDP-N-acetylgalactosamine pyrophosphorylase (AGX1). Bioorganic & Medicinal Chemistry Letters, 22 (12) 3957-3961. 

4.      Min Fang, Li Gu, Guofeng Gu*, Junqiang Fang. 2012. Facile Synthesis and Antitumor Activities of Timosaponin AIII and Its Analogs. Journal of Carbohydrate Chemistry, 31( 3): 187-202.

3.      Yafei Zhai, Min Liang, Junqiang Fang, Xiaoyue Wang, Wanyi Guan, Xian-wei Liu*, Peng Wang, Fengshan Wang. 2012. NahK/GlmU fusion enzyme: Characterization and one-step enzymatic synthesis of UDP-N-acetylglucosamine. Biotechnology Letters, 34(7): 1321-1326.

2.      Wanyi Guan^†, Li Cai^†, Junqiang Fang^†, Baolin Wu, Peng George Wang*. 2009. Enzymatic synthesis of UDP-GlcNAc/UDP-GalNAc analogs using N-acetylglucosamine 1-phosphate uridyltransferase (GlmU). Chemical Communications, (45): 6976-6978.

1.    Junqiang Fang, Wanyi Guan, Li Cai, Guofeng Gu*, Xianwei Liu*, Peng George Wang. 2009. Systematic study on the broad nucleotide triphosphate specificity of the pyrophosphorylase domain of the N-acetylglucosamine-1-phosphate uridyltransferase from Escherichia coli K12. Bioorganic & Medicinal Chemistry Letters, 19(22): 6429-6432.

专著章节：

1.       Mohui Wei, Zijie Li, Baolin Wu, Yunpeng Liu, Tiehai Li, Liuqing Wen, Jing Li, Jiansong Cheng, Junqiang Fang, Xianwei Liu, Peng George Wang. Chapter 6: Dihydroxyacetone Phosphate-Dependent Aldolases: From Flask Reaction to Cell-Based Synthesis. Handbook of Carbohydrate-Modifying Biocatalysts. Pages 275–296 Print ISBN: 978-981-4669-78-8 eBook ISBN: 978-1-351-73398-4.

2.         Guohui Zhao , Yunpeng Liu, Zhigang Wu, Hailiang Zhu, Zaikuan Yu, Junqiang Fang , Peng  George Wang. Chapter 52 Chemoenzymatic Synthesis of Glycoproteins. Glycoscience: Biology and Medicine