山东省海洋原位传感器工程技术协同创新中心

Extensible LED-Induced Integrated Fluorescence Detection Module for Quantitative Analysis of Lucigenin Concentration

  • 发表刊物: Photonics
  • 关键字: extensible; LED-induced; ultra-compact; fluorescence detection; lucigenin concentration;quantitative analysis
  • 是否译文:
  • 发表时间: 2023-04-01
  • 收录刊物: SCI
摘要: We developed an extensible LED-induced fluorescence detection module with a highly integrated and ultra-compact structure. A target-oriented design methodology was used to demonstrate the module’s optimal design. Lucigenin solution was used as a test sample in evaluation trials to demonstrate the module’s quantitative fluorescence detection capability. Results showed that the integrated module has an outstanding linear response in the range of 0–1 µmol·L−1, with sensitivity and limit of detection (LOD) of 0.1692 V/µmol·L−1 and 0.03 µmol·L−1, respectively. Statistical analyses showed that our integrated module has extremely high repeatability and accuracy, i.e., the values of Pearson’s correlation coefficient and root-mean-square error exceeded 0.9995 and 1.8‰, respectively. More importantly, the integrated module possesses favorable extensibility and can realize on-demand rapid fluorescence-signal detection of other targets using appropriate parameter combinations. This module offers new opportunities for reliable, cost-effective and easy-to-use fluorescence-signal detection, especially in resource-constrained fluorescence detection applications.