刘玲
助理研究员
所属院部: 海洋学院
访问次数:
论文成果
Cytotoxicity of the soluble and insoluble fractions of atmospheric fine particulate matter
  • 所属单位:
    ​山东大学环境研究所
  • 教研室:
    ​山东大学环境研究所
  • 发表刊物:
    Journal of Environmental Sciences
  • 刊物所在地:
    中国
  • 关键字:
    PM2.5;Soluble fraction;Insoluble fraction;Membrane rupture;Reactive oxygen species
  • 摘要:
    Inhaled atmospheric fine particulate matter (PM2.5) includes soluble and insoluble fractions, and each fraction can interact with cells and cause adverse effects. PM2.5 samples were collected in Jinan, China, and the soluble and insoluble fractions were separated. According to physiochemical characterization, the soluble fraction mainly contains water-soluble ions and organic acids, and the insoluble fraction mainly contains kaolinite, calcium carbonate and some organic carbon. The interaction between PM2.5 and model cell membranes was examined with a quartz crystal microbalance with dissipation (QCM-D) to quantify PM2.5 attachment on membranes and membrane disruption. The cytotoxicity of the total PM2.5 and the soluble and insoluble fractions, was investigated. Negatively charged PM2.5 can adhere to the positively charged membranes and disrupt them. PM2.5 also adheres to negatively charged membranes but does not cause membrane rupture. Therefore, electrostatic repulsion does not prevent PM2.5 attachment, but electrostatic attraction induces remarkable membrane rupture. The human lung epithelial cell line A549 was used for cytotoxicity assessment. The detected membrane leakage, cellular swelling and blebbing indicated a cell necrosis process. Moreover, the insoluble PM2.5 fraction caused a higher cell mortality and more serious cell membrane damage than the soluble fraction. The levels of reactive oxygen species (ROS) enhanced by the two fractions were not significantly different. The findings provide more information to better understand the mechanism of PM2.5 cytotoxicity and the effect of PM2.5 solubility on cytotoxicity.
  • 全部作者:
    Qiuhua Zhou,Xuezhi Yang,Gang Li,Jingzhu Zhang,Xuehua Zhou 
  • 第一作者:
    Ling Liu
  • 论文类型:
    研究论文
  • 通讯作者:
    Wei Jiang
  • 文献类型:
    期刊论文
  • 卷号:
    91
  • 页面范围:
    105-116
  • ISSN号:
    1001-0742​
  • 是否译文:
  • 发表时间:
    2020-05-01
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