LIU Xianwei   

Title:Associate Professor
Supervisor of Master's Candidates

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Language:English

Profile

刘现伟,山东大学,国家糖工程技术研究中心,副教授

教育经历:

2003/09-2009/06,山东大学,生命科学学院,博士(硕博连读),导师:王鹏教授

1999/09-2003/06,山西大学,生命科学学院,学士

科研与学术工作经历:

2010/07-至今,山东大学,国家糖工程技术研究中心

2009/07-2010/06,山东大学,药学院,师资博士后,合作导师:王凤山教授

2007/02-2009/03,美国俄亥俄州立大学,生物化学和化学院,访问学者

主持或参加科研项目(课题)及人才计划项目情况(按时间倒序排序):

1 科技部,国家重点研发计划“合成生物学”专项课题,2018YFA0902004,以改变给药途径为导向的合成生物学创制(课题4;所属项目:2018YFA0902000,基于成药性特征的微生物天然产物合成生物学创制;负责人:陈依军 中国药科大学),2019-07 2024-0646万元,在研,参加(子课题负责人)

2)国家自然科学基金委员会,面上项目,21778035,新型O-乙酰葡糖胺-6-磷酸蛋白糖基化的检测、过程机理及生物功能研究,2018-012021-1265万元,在研,主持

3)山东省自然科学基金委员会,面上项目,ZR2016BM13,蛋白氧连接乙酰葡糖胺-6-磷酸修饰的检测及功能研究,2016-112019-0615万元,结题,主持

4)国家自然科学基金委员会,面上项目,21372145,氟代肿瘤相关糖抗原的化学酶法合成及其在肿瘤糖疫苗中的应用,2014-012017-1280万元,结题,参加(第2位)

5)山东省自然科学基金委员会,山东省优秀中青年科学家科研奖励基金,BS2013SW023,细菌蛋白O-寡糖基转移酶研究及其在新型糖疫苗研制中的应用,2013-102015-105万元,结题,主持

6)科技部,国家高技术研究发展计划(863计划)课题,2012AA021504,“糖工程关键技术与重大产品开发”项目之课题4“糖链修饰与糖衍生物的研制”,2012-012015-12200万元,结题,参加(第7位)

7)科技部,国家重大新药创制专项课题,2012ZX09502001-005,糖疫苗及相关技术体系的建立与研究,2012-012015-12400万元,结题,参加(第7位)

8)科技部,国家重点基础研究发展计划(973计划)课题,2012CB822102,“肿瘤的糖化学生物学前沿研究”项目之课题2“糖链探针的生物合成研究”,2012-012016-10595万元,结题,参加(第11位)

9 国家自然科学基金委员会,青年科学基金项目,31000369,一种植物来源的双功能蛋白N-糖苷酶/转谷氨酰胺酶的研究及其在合成糖蛋白中的应用,2011-012013-1220万元,结题,主持

10)中国博士后科学基金,20090461238,酶法合成岩藻糖基化药用寡糖及其类似物,2010-012012-125万元,结题,主持

代表性研究成果

一、期刊论文

1. 第一作者论文

(1) Jinfeng Ye#, Xian-wei Liu#, Peng Peng, Wen Yi, Xi Chen, Fengshan Wang, Hongzhi Cao*. 2016. Diversity-Oriented Enzymatic Modular Assembly of ABO Histo-blood Group Antigens. ACS Catalysis, 6 (12): 8140-8144.

(2) Xian-wei Liu, Chengfeng Xia, Lei Li, Wan-yi Guan, Nicholas Pettit, Hou-cheng Zhang, Min Chen*, Peng George Wang. 2009. Characterization and synthetic application of a novel β1,3-galactosyltransferase from Escherichia coli O55:H7. Bioorganic & Medicinal Chemistry, 17(14): 4910-4915.

2. 通讯作者论文

(1) Jing Liu, Xuefei Yin, Zitao Li, Xiaocong Wu, Zhaoxuan Zheng, Junqiang Fang, Guofeng Gu, Peng G. Wang, Xianwei Liu*. 2021. Facile enzymatic synthesis of diverse naturally-occurring β-D-mannopyranosides catalyzed by glycoside phosphorylases. ACS Catalysis, 11(5): 2763-2768.

(2) Wenjing Shang, Yafei Zhai, Zhongrui Ma, Gongjin Yang, Yan Ding, Donglei Han, Jiang Li, Houcheng Zhang, Jun Liu, Peng George Wang, Xian-wei Liu*, Min Chen*, 2016. Production of human blood group B antigen epitope conjugated protein in Escherichia coli and utilization of the adsorption blood group B antibody. Microbial Cell Factories, 15: 138.

(3) Yafei Zhai, Donglei Han, Ying Pan, Shuaishuai Wang, Junqiang Fang, Peng Wang, Xian-wei Liu*. 2015. Enhancing GDP-fucose production in recombinant Escherichia coli by metabolic pathway engineering. Enzyme and Microbial Technology, 69: 38-45.

(4) Junqiang Fang, Mengyang Xue, Guofeng Gu, Xian-wei Liu*, Peng George Wang. 2013. A chemoenzymatic route to synthesize unnatural sugar nucleotides using a novel N-acetylglucosamine-1-phosphate pyrophosphorylase from Camphylobacter jejuni NCTC 11168. Bioorganic & Medicinal Chemistry Letters, 23(15): 4303-4307.

(5) Mengyang Xue, Wanyi Guan, Yang Zou, Junqiang Fang*, Xian-wei Liu*, Peng George Wang, Fengshan Wang. 2012. Investigation of the nucleotide triphosphate substrate specificity of Homo sapiens UDP-N-acetylgalactosamine pyrophosphorylase (AGX1). Bioorganic & Medicinal Chemistry Letters, 22 (12): 3957-3961.

(6) Yafei Zhai, Min Liang, Junqiang Fang, Xiaoyue Wang, Wanyi Guan, Xian-wei Liu*, Peng Wang, Fengshan Wang. 2012. NahK/GlmU fusion enzyme: Characterization and one-step enzymatic synthesis of UDP-N-acetylglucosamine. Biotechnology Letters, 34(7): 1321-1326.

(7) Min Liang, Min Chen, Xinying Liu, Yafei Zhai, Xian-wei Liu*, Houcheng Zhang*, Min Xiao, Peng Wang. 2012. Bioconversion of D-galactose to D-tagatose: continuous packed bed reaction with an immobilized thermostable L-arabinose isomerase and efficient purification by selective microbial degradation. Applied Microbiology and Biotechnology, 93(4): 1469-1474.

(8) Junqiang Fang, Wanyi Guan, Li Cai, Guofeng Gu*, Xianwei Liu*, Peng George Wang. 2009. Systematic study on the broad nucleotide triphosphate specificity of the pyrophosphorylase domain of the N-acetylglucosamine-1-phosphate uridyltransferase from Escherichia coli K12. Bioorganic & Medicinal Chemistry Letters, 19(22): 6429-6432.

3. 既非第一作者又非通讯作者论文

(1) Jinfeng Ye, Hui Xia, Na Sun, Chang-Cheng Liu, Anran Sheng, Lianli Chi, Xian-Wei Liu, Guofeng Gu, Shu-Qi Wang, Jie Zhao, Ping Wang, Min Xiao, Fengshan Wang, Hongzhi Cao*. 2019. Reprogramming the enzymatic assembly line for site-specific fucosylation. Nature Catalysis, 2(6): 514-522.

(2) Liuqing Wen*, Garrett Edmunds, Christopher Gibbons, Jiabin Zhang, Madhusudhan Reddy Gadi, Hailiang Zhu, Junqiang Fang, Xianwei Liu, Yun Kong, and Peng George Wang*. 2018. Toward Automated Enzymatic Synthesis of Oligosaccharides. Chemical Reviews, 118(17): 8151-8187.

(3) Shuaishuai Wang, Xuan Fu, Yunpeng Liu, Xian-wei Liu, Lin Wang, Junqiang Fang*, Peng George Wang. 2015. Probing the roles of conserved residues in uridyltransferase domain of Escherichia coli K12 GlmU by site-directed mutagenesis. Carbohydrate Research, 413: 70-74

(4) Wanyi Guan, Lan Ban, Li Cai, Lei Li, Wenlan Chen, Xianwei Liu, Milan Mrksich, Peng George Wang*. 2011. Combining carbochips and mass spectrometry to study the donor specificity for the Neisseria meningitidis β1,3-N-acetylglucosaminyl-transferase LgtA. Bioorganic & Medicinal Chemistry Letters, 21(17): 5025-5028.

(5) Robert Woodward, Wen Yi, Lei Li, Guohui Zhao, Hironobu Eguchi, Perali Ramu Sridhar, Hongjie Guo, Jing Katherine Song, Edwin Motari, Li Cai, Patrick Kelleher, Xianwei Liu, Weiqing Han, Wenpeng Zhang, Yan Ding, Mei Li, Peng George Wang*. 2010. In vitro bacterial polysaccharide biosynthesis: defining the functions of Wzy and Wzz. Nature Chemical Biology, 6(6): 418-423.

(6) Lei Li, Robert Woodward, Yan Ding, Xian-wei Liu, Wen Yi, Veer S. Bhatt, Min Chen, Lian-wen Zhang, Peng George Wang*. 2010. Overexpression and topology of bacterial oligosaccharyltransferase PglB. Biochemical and Biophysical Research Communications, 394(4): 1069-1074.

(7) Wen Yi, Xianwei Liu, Yanhong Li, Jianjun Li, Chengfeng Xia, Guangyan Zhou, Wenpeng Zhang, Wei Zhao, Xi Chen, Peng George Wang*. Remodeling bacterial polysaccharides by metabolic pathway engineering. 2009. Proceedings of the National Academy of Sciences of the United States of America, 106(11): 4207-4212.

二、专著章节

(1) Xian-wei Liu, Lei Li, Hai-tao Gao, Li-jun Dang, Peng G. Wang, Chapter 15 Synthesis and Modification of Carbohydrates via Metabolic Pathway Engineering in Microorganisms, in Carbohydrate-Modifying Biocatalysts (Edited by Peter Grunwald), pp 657–682, Pan Stanford Publishing Pte. Ltd., 2012


Educational Experience

  • 2003.9-2009.6  

    Shandong University       Microbiology       Graduate class completion       Doctoral Degree in Science

  • 1999.9-2003.7  

    Shanxi University       Biology       University graduated       Bachelor's Degree in Science

  • 1996.9-Now  

    山东省兖州市第一中学

Work Experience

  • 2009.7-Now

    山东大学国家糖工程技术研究中心

  • 2009.7-Now

    Shandong University      National Glycoengineering Research Center      Associate Professor

  • 2007.2-2009.2

    The Ohio State University      Department of Biochemistry and Chemistry      Visiting Scholar

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