论文成果
Oocyte-dependent activation of MTOR in cumulus cells controls the development and survival of cumulus-oocyte complexes.
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发表刊物：
J Cell Sci
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刊物所在地：
UK
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关键字：
Apoptosis; Cumulus cells; DDIT4L; Female infertility; MTOR; Oocyte
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摘要：
Communication between oocytes and their companion somatic cells promotes the healthy development of ovarian follicles, which is crucial for producing oocytes that can be fertilized and are competent to support embryogenesis. However, how oocyte-derived signaling regulates these essential processes remains largely undefined. Here, we demonstrate that oocyte-derived paracrine factors, particularly GDF9 and GDF9-BMP15 heterodimer, promote the development and survival of cumulus-cell-oocyte complexes (COCs), partly by suppressing the expression of Ddit4l, a negative regulator of MTOR, and enabling the activation of MTOR signaling in cumulus cells. Cumulus cells expressed less Ddit4l mRNA and protein than mural granulosa cells, which is in striking contrast to the expression of phosphorylated RPS6 (a major downstream effector of MTOR). Knockdown of Ddit4l activated MTOR signaling in cumulus cells, whereas inhibition of MTOR in COCs compromised oocyte developmental competence and cumulus cell survival, with the latter likely to be attributable to specific changes in a subset of transcripts in the transcriptome of COCs. Therefore, oocyte suppression of Ddit4l expression allows for MTOR activation in cumulus cells, and this oocyte-dependent activation of MTOR signaling in cumulus cells controls the development and survival of COCs.
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全部作者：
Lanying Shi, Xuhong Gong, Mengjie Jiang, Yaoxue Yin, Xiaoyun Zhang, Hong Yin, Hui Li, Chihiro Emori, Koji Sugiura, John J Eppig
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第一作者：
Jing Guo
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论文类型：
Research Article
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通讯作者：
You-Qiang Su
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论文编号：
10.1242/jcs.182642
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卷号：
129
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期号：
16
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页面范围：
3091-103
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ISSN号：
1477-9137
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是否译文：
否
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发表时间：
2016-08-15
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下一条：Ribonuclease activity of MARF1 controls oocyte RNA homeostasis and genome integrity in mice.
