Affiliation of Author(s):山东大学
Teaching and Research Group:谭保才课题组
Journal:Plant Communications
Place of Publication:中国
Funded by:国家自然科学基金
Key Words:maizeplant organellesRNA editingZmNUWAZmDYW2A/2B
Abstract:RNA C-to-U editing is essential for plant organellar gene expression. It is proposed that PPR-E+ proteins bind to target sites and recruit cytidine deaminase AtDYW2 facilitated by AtNUWA. We analyzed the function of ZmNUWA, ZmDYW2A, and ZmDYW2B and their relationship with other editing factors in maize. The zmdyw2a and zmdyw2b single mutants are normal, but the zmdyw2a::zmdyw2b and zmnuwa mutants are severely arrested in seed development. ZmNUWA, ZmDYW2A, and ZmDYW2B are dual-localized in mitochondria and plastids. Loss of ZmNUWA decreases the editing at 99 mitochondrial sites and 8 plastid sites. Surprisingly, loss of ZmDYW2A:ZmDYW2B affects almost the same set of sites targeted by PPR-E+ proteins. ZmNUWA interacts with ZmDYW2A and ZmDYW2B, suggesting that ZmNUWA recruits ZmDYW2A/2B in the editing of PPR-E+ protein targeted sites in maize. Further protein interaction analyses show that ZmNUWA and ZmDYW2A/2B interact with ZmMORF1, ZmMORF8, ZmMORF2, and ZmMORF9; and ZmOZ1 interacts with ZmORRM1, ZmDYW2A, ZmDYW2B, ZmMORF8, and ZmMORF9. These results suggest that the mitochondrial PPR-E+ editosome contains PPR-E+, ZmDYW2A/2B, ZmNUWA, and ZmMORF1/8, whereas the plastid PPR-E+ editosome composes of PPR-E+, ZmDYW2A/2B, ZmNUWA, ZmMORF2/8/9, ZmORRM1 and ZmOZ1 in maize.
All the Authors:Huang Zi-Qin,Tian Kai-Di,Li Hao,Xu Chunhui,Xia Bingyujie
First Author:Wang Yong
Indexed by:Journal paper
Correspondence Author:Tan Bao-Cai
Discipline:Natural Science
First-Level Discipline:Biology
Translation or Not:no
Date of Publication:2024-02-01
Included Journals:SCI
Date of Publication:2024-02-01