Affiliation of Author(s):山东大学
Teaching and Research Group:谭保才教授
Journal:New Phytol.
Funded by:国家自然科学基金
Key Words:Rrp15p; maize DEK58; pre-rRNA processing; ribosome biogenesis; seed development
Abstract:Ribosome biogenesis is a highly dynamic and orchestrated process facilitated by hundreds of ribosomal biogenesis factors and small nucleolar RNAs. While many of the advances are derived from studies in yeast, ribosome biogenesis remains largely unknown in plants despite its importance to plant growth and development. Through characterizing the maize (Zea mays) defective kernel and embryo-lethal mutant dek58, we show that DEK58 encodes an Rrp15p domain-containing protein with 15.3% identity to yeast Rrp15. Over-expression of DEK58 rescues the mutant phenotype. DEK58 is localized in the nucleolus. Ribosome profiling and RNA gel blot analyses show that the absence of DEK58 reduces ribosome assembly and impedes pre-rRNA processing, accompanied by the accumulation of nearly all the pre-rRNA processing intermediates and the production of an aberrant processing product P-25S*. DEK58 interacts with ZmSSF1, a maize homolog of the yeast Ssf1 in the 60S processome. DEK58 and ZmSSF1 interact with ZmCK2α, a putative component of the yeast UTP-C complex involved in the small ribosomal subunit processome. These results demonstrate that DEK58 is essential to seed development in maize. It functions in the early stage of pre-rRNA processing in ribosome biogenesis, possibly through interacting with ZmSSF1 and ZmCK2α in maize.
All the Authors:Xiu Zhi-hui,Yang Huan-huan,Wang Hongqiu,Wang Yong
First Author:Ma Bing,Liu Hui
Indexed by:Journal paper
Correspondence Author:Tan Bao-Cai
Discipline:Natural Science
First-Level Discipline:Biology
Translation or Not:no
Date of Publication:2024-01-01
Included Journals:SCI
Date of Publication:2024-01-01