1. 微生物活性代谢产物的挖掘与生物合成机制研究
以活性和基因组信息为导向，挖掘微生物源活性天然产物，通过生物信息学、基因工程技术、代谢组学、功能酶学等手段解析天然产物的生物合成机制；利用合成生物学等方法改造天然产物生物合成途径及分子结构，深度挖掘微生物的代谢潜能。
2. 微生物基因组编辑与应用
以前沿的分子克隆和DNA编辑技术为基础，开发细菌、噬菌体等微生物基因组组装和编辑新技术，拓展微生物的应用价值。
暂无内容
1. Gu Yuan#, Wang Zong-Jie#, Wang Huimei#, Su Anqi, Dai Quan, Zhang Youming, Huo Liujie*, Yan Fu*. Biosynthetic Investigations of Ulbactins Unveil Two Novel Thiazolinyl Imine Reductases Crucial for the Generation of Siderophore Activity. Journal of Agricultural and Food Chemistry, 73, 8352−8366, 2025.
2. Han Liyuan, Xu Jiaxin, Gu Yuan, Feng Ruiying, Liu Shuo, Lu Qiujie, Lv Shuzhe, Zhang Youming * and Yan Fu*. Teichoic acid biosynthesis enhances conjugation efficiency in Streptomyces: A key to unlocking DNA delivery into industrially relevant strains. International Journal of Biological Macromoleclues, 307, 142248, 2025.
3. Feng Ruiying,# Dai Quan,# Ma Meina,# Zhao Shuang, Wang Zongjie, Wang Huimei, Zhang Youming*, Huo Liujie*, and Yan Fu*. Discovery of Ureido-Containing Alteropeptilides and Intramolecular Cyclized Alteramides in Pseudoalteromonas flavipulchra S16 by Promoter Engineering of Cryptic Biosynthetic Gene Clusters. Journal of Natural Product, 87, 2801-2809, 2024.
4. Cong, Xiaomei, Zhao Shuang, Zhang Qing, Liu Shuo, Zhang Youming*, and Yan Fu*. Isolation, Characterization, and Genome Engineering of a Lytic Pseudomonas aeruginosa Phage. Microorganisms, 12, 2346, 2024.
5. Liu Yang,† Zhou Haibo,† Zhao Shuang, Hao Xingkun, Dai Guangzhi, Zhong Lin, Ren Xiangmei, Sui Haiyan, Zhang Youming *, Yan Fu *. Biosynthesis of trans-AT PKS-Derived Shuangdaolides Featuring a trans-acting Enzyme for Online Epoxidation. ACS Chemical Biology, 18, 2474, 2023.
6. Zhao Shuang,† Feng Ruiying,† Gu Yuan,† Han Liyuan, Cong Xiaomei, Liu Yang, Liu Shuo, Shen Qiyao, Huo Liujie, Yan Fu*. Heterologous expression facilitates the discovery and characterization of marine microbial natural products. Engineering Microbiology, 100137, 2023.
7. Wang Zong-Jie,† Liu Xiaotong,† Zhou Haibo,† Liu Yang, Tu Qiang, Huo Liujie, Yan Fu, Müller Rolf, Zhang Youming,* Xu Xiaokun*. Engineered Biosynthesis of Complex Disorazol Polyketides in a Streamlined Burkholderia thailandensis. ACS Synthetic Biology, 12, 971-977, 2023.
8. Liu Renzong, Sun Yi, Ma Meina, Han Liyuan, Gao Sheng, Zhang Youming, Yan Fu*. Aurantimonas marianensis sp. nov., isolated from deep-sea sediment of the Mariana Trench. International Journal of Systematic and Evolutionary Microbiology, 73, 005816, 2023.
9. Zhang Songya,† Chen Yunliang,† Zhu Jing, Lu Qiujie, Cryle Max J.,* Zhang Youming *, Yan Fu*. Structural diversity, biosynthesis, and biological functions of lipopeptides from Streptomyces. Natural Product Reports, 40, 557, 2023.
10. Liu Zhiguo, Yashiroda Yoko, Sun Peng, Ma Hai, Wang Yanan, Li Li, Yan Fu*, Sun Yi*. Argenteolides A and B, Glycosylated Polyketide-Peptide Hybrid Macrolides from an Actinomycete Streptomyces argenteolus. Organic Letters, 25, 571-575, 2022.
11. Zhong Guannan,† Wang Zong-Jie,† Yan Fu, Zhang Youming, Huo Liujie*. Recent advances in discovery, bioengineering and bioactivity-evaluation of ribosomally synthesized and post-translationally modified peptides. ACS BIO&MED CHEM Au, 2022.
12. Chen Hanna, Sun Tao, Bai Xianping, Yang Jie, Yan Fu, Yu Lei, Tu Qiang, Li Aiying, Tang Yajie, Zhang Youming,* Bian Xiaoying * and Zhou Haibo*. Genomics-Driven Activation of Silent Biosynthetic Gene Clusters in Burkholderia gladioli by Screening Recombineering System. MOLECULES, 2021.
13. 李瑞娟，赵晓雨，杨润雨，刘洋，颜富，王海龙，张友明，符军*. 噬菌体重组酶介导的DNA 同源重组工程. 微生物学通报, 2021.
14. Zhang Chao, Yu Jing, Tu Qiang, Yan Fu, Hu Zhao, Zhang Youming*, Song Chun*. Antioxidant Capacities and Enzymatic Inhibitory Effects of Different Solvent Fractions and Major Flavones from Celery Seeds Produced in Different Geographic Areas in China. Antioxidants (Basel, Switzerland), 11, 2022.
15. Zhang Jingdan, Xu Huizhong, Yang Hu, Li Jia, Xiao Shuai, Hu Shengbiao, Yan Fu, Xia Liqiu*, Zhang Youming*. Screening of a Plesiomonas shigelloides phage and study of the activity of its lysis system.. Virus Research, 306, 198581, 2021.
16. Wang Zong-Jie†, Liu Xiaotong†, Zhou Haibo†, Liu Yang, Zhong Lin, Wang Xue, Tu Qiang, Huo Liujie, Yan Fu, Gu Lichuan, Müller Rolf, Zhang Youming*, Bian Xiaoying*, Xu Xiaokun*. Engineering of Burkholderia thailandensis strain E264 serves as a chassis for expression of complex specialized metabolites. Frontiers in Microbiology, 2022.
17. Liu Yang, Zhou Haibo, Shen Qiyao, Dai Guangzhi, Yan Fu, Li Xiaoju, Ren Xiangmei, Sun Qin, Tang Ya-Jie , Zhang Youming, Bian Xiaoying*. Discovery of Polycyclic Macrolide Shuangdaolides by Heterologous Expression of a Cryptic trans-AT PKS Gene Cluster. Organic Letters, 23, 6967, 2021.
18. Yan Fu and Müller Rolf*. Class I methyltransferase VioH catalyzes unusual SAM cyclization leading to 4-methylazetidinecarboxylic acid formation in vioprolide biosynthesis. ACS Chemical Biology, 14, 99, 2019.
19. Auerbach David,# Yan Fu,# Zhang Youming and Müller Rolf*. Characterization of an unusual glycerate esterification process in vioprolide biosynthesis. ACS Chemical Biology, 13, 3123, 2018.
20. Yan Fu,# Burgard Christian,# Popoff Alexander, Zaburannyi Nestor, Zipf Gregor, Maier Josef, Bernauer S. Hubert, Wenzel C. Silke, Müller Rolf*. Synthetic biology approaches and combinatorial biosynthesis towards heterologous lipopeptide production. Chemical Science, 9, 7510, 2018.
21. Yan Fu,# Auerbach David,# Chai Yi, Keller Lena, Tu Qiang, Hüttel Stephan, Glemser Ameli, Grab A. Hanusch, Bach Thorsten, Zhang Youming,* Müller Rolf*. Biosynthesis and Heterologous Production of Vioprolides: Rational Biosynthetic Engineering and Unprecedented 4-Methylazetidinecarboxylic Acid Formation. Angewandte Chemie-International Edition, 57, 8754, 2018.
22. Yin Jia, Hoffmann Michael, Bian Xiaoying, Tu Qiang, Yan Fu, Xia Liqiu, Ding Xuezhi, Stewart A. Francis, Müller Rolf, Fu Jun, Zhang Youming*. Direct cloning and heterologous expression of the salinomycin biosynthetic gene cluster from Streptomyces albus DSM41398 in Streptomyces coelicolor A3(2). Scientific Reports, 5, 2015.
23. Bian Xiaoying, Plaza Alberto, Yan Fu, Zhang Youming, Müller Rolf*. Rational and efficient site-directed mutagenesis of adenylation domain alters relative yields of luminmide. Biotechnology and Bioengineering, 112, 1343, 2015.
24. Yan Fu, Cheng Xing, Ding Xuezhi, Yao Ting, Chen Hanna, Li Wenping, Hu Shengbiao, Yu Ziquan, Sun Yunjun, Zhang Youming, Xia Liqiu*. Improved insecticidal toxicity by fusing Cry1Ac of Bacillus thuringiensis with Av3 of Anemonia viridis. Current Microbiology, 68, 604, 2013.
1. 微生物活性多肽的定向挖掘与合成生物学改造, 2024/01/01-2026/12/31
2. 微生物次级代谢混合能力挖掘：细菌杂合萜烯类天然产物生物合成, 2022/01/01-2024/12/31
3. 工业微生物基因组大片段DNA的设计原理与操纵方法, 2021/06/21-2024/06/30
4. 厌氧梭菌活性天然产物的发现及生物合成机制研究, 2020/09/18-2023/12/31
5. 基因组重编程实现噬菌体精准控制, 2020/01/01-2024/12/31
