|
个人信息Personal Information
教授 博士生导师 硕士生导师
性别：男
毕业院校：德国德累斯顿工业大学/山东大学/湖南师范大学
学历：研究生(博士后)
学位：博士后
在职信息：在职
所在单位：微生物技术研究院（微生物改造技术全国重点实验室）
入职时间：2013-06-03
学科：微生物学
1. Liu, Y. # and Wang, H.*, Reply to: Docking domains from modular polyketide synthases and their use in engineering. Nature Communications, 2025. 16(1). （独立通讯作者corresponding author，IF=17.2，综合性一区Top期刊）
2. Jiang, C. #; Hao, K.; Wang, S.; Guo, R.; Li, X.; Lv, L.; Luan, J.; Wang, H.*, Developing the protein quality control system in Streptomyces for selective translation of ultra-long full-length polyketide synthase mRNAs to enhance biosynthesis efficiency. Synthetic and Systems Biotechnology. 已接收（独立通讯作者corresponding author，IF=4.5，生物工程与应用微生物一区Top期刊）
3. Cui, T. #; Jiang, C.; Li, J.; Wang, S.; Li, L.; Luan, J.; Wang, H.*, Modular engineering of the doxorubicin biosynthetic gene cluster based on component functional clustering in Streptomyces. Journal of Biotechnology, 2025. 408: p. 265-274.（独立通讯作者corresponding author，IF=3.8，生物学二区）
4. Guan, Z. #; Wang, H.* and Feng, Q.*, Establishment and improvement of genetic manipulation tools for Fusobacterium nucleatum. Engineering Microbiology, 2025. 5(1): p. 100192.（并列通讯作者co-corresponding author）
5. Zhang, H. #; Zhu, R.; Wang, Z.; He, R.; Zhang, Y.; Luan, J.; Yan, Y.; Zhang, Y.; Wang, H.*, Programming virulent bacteriophages by developing a multiplex genome engineering method. mBio, 2025, p. e0358224.（独立通讯作者corresponding author，IF=5.5，微生物学二区Top期刊）
6. Liu, Y. #; Song, C.; Cui, Q.; Sun, H.; Jiang, C.; Guo, R.; He, R.; Li, Z.; Luan, J.; & Wang, H.*, Improving polyketide biosynthesis by rescuing the translation of truncated mRNAs into functional polyketide synthase subunits, Nature Communications, 2025, 16(1).（独立通讯作者corresponding author，IF=17.2，综合性一区Top期刊）
7. Yang, Y. #; Yuan, H.; Zhang, Y.; Luan, J.; & Wang, H.*, Progress in African swine fever vector vaccine development, International Journal of Molecular Sciences, 2025, 26(3):921.（独立通讯作者corresponding author，IF=5.7，生化与分子生物学三区）
8. Luan, J. #; Song, C.; Liu, Y.; He, R.; Guo, R.; Cui, Q.; Jiang, C.; Li, X.; Hao, K.; Stewart, A. F.; Fu, J.; Zhang, Y. & Wang, H.*, Seamless site-directed mutagenesis in complex cloned DNA sequences using the RedEx method, Nature Protocols, 2024, 19(11), 3360-3388.（独立通讯作者corresponding author，IF=19.4，生化研究方法一区Top期刊）
9. Yu, G. #; Duan, Q.; Cui, T.; Jiang, C.; Li, X.; Li, Y.; Fu, J.; Zhang, Y.;Wang, H.* & Luan, J.*, Development of a bacterial gene transcription activating strategy based on transcriptional activator positive feedback, Journal of Advanced Research, 2023, 66, 155-164.（并列通讯作者co-corresponding author，IF=11.6，综合性一区Top期刊）
10. Li, X. #; Guo, R.; Luan, J.; Fu, J.; Zhang, Y. & Wang, H.*, Improving spinosad production by tuning expressions of the forosamine methyltransferase and the forosaminyl transferase to reduce undesired less active byproducts in the heterologous host Streptomyces albus J1074, Microbial Cell Factories, 2023, 22(1), 15.（独立通讯作者corresponding author，IF=6.1，生物工程与应用微生物二区）
11. Jiang, C. #; She, Q. & Wang, H.*, Editorial: Insights in genome editing tools and mechanisms: 2022. Frontiers in Genome Editing, 2023, 5, 1240576.（独立通讯作者corresponding author，IF=4.9，生物工程与应用微生物二区）
12. 李雨桐Li, Y. #; 崔天琦Cui, T.; 张海林Zhang, H.; 于广乐Yu, G.; 栾霁Luan, J.*&王海龙Wang, H.*，肿瘤靶向细菌Escherichia coli Nissle 1917在癌症治疗中的研究进展Research progress of tumor-targeting bacteria Escherichia coli Nissle 1917 in cancer treatment，中国生物工程杂志China Biotechnology，2023, 43(6): 54-68.（并列通讯作者co-corresponding author，in Chinese）
13. 姜婵娟Jiang, C. #; 崔天琦Cui, T.; 孙洪娈Sun, H.; 焦念志Jiao, N.; 符军Fu, J.; 张友明Zhang, Y.*; 王海龙Wang, H.*，ExoCET-BAC策略高效抓取和组装高AT含量基因组大片段Efficient capture and assembly of AT-rich genomic fragments using ExoCET-BAC strategy，合成生物学Synthetic Biology Journal，2022, 3(1): 238-251.（末位并列通讯作者co-corresponding author, in Chinese） 获得《合成生物学》期刊2024年度优秀论文
14. Jiang, T. #,*; Yang, X. #; Li, G.; Zhao, X.; Sun, T.; Muller, R.; Wang, H.*; Li, M.* & Zhang, Y.*, Bacteria-based live vehicle for in vivo bioluminescence imaging, Analytical Chemistry, 2021, 93(47), 15687-15695.（并列通讯作者co-corresponding author，IF=6.6，化学一区Top期刊）
15. Tang, Y. #; Zhang, C.; Cui, T.; Lei, P.; Guo, Z. *; Wang, H.*& Liu, Q.*, The discovery of actinospene, a new polyene macrolide with broad activity against plant fungal pathogens and pathogenic yeasts, Molecules, 2021, 26(22).（并列通讯作者co-corresponding author，IF=5.0，生化与分子生物学三区）
16. Song, C.#; Luan, J.#; Li, R.; Jiang, C.; Hou, Y.; Cui, Q.; Cui, T.; Tan, L.; Ma, Z.; Tang, Y. J.; Stewart, A. F.; Fu, J.; Zhang, Y. & Wang, H.*, RedEx: a method for seamless DNA insertion and deletion in large multimodular polyketide synthase gene clusters, Nucleic Acids Research, 2020, 48(22), e130.（独立通讯作者corresponding author，IF=16.8，生化与分子生物学二区Top期刊）
17. Jiang, C. #; Zhou, H; Sun, H; He, R; Song, C; Cui, T; Luan, J; Fu, J; Zhang, Y; Jiao, N; Wang, H.*, Establishing an efficient salinomycin biosynthetic pathway in three heterologous Streptomyces hosts by constructing a 106-kb multioperon artificial gene cluster, Biotechnology and Bioengineering, 2021, 118: 4668-4677.（独立通讯作者corresponding author，IF=3.8，生物工程与应用微生物三区）
18. Abbasi, M. N. #; Fu, J.; Bian, X.; Wang, H.*; Zhang, Y.* & Li, A.*, Recombineering for genetic engineering of natural product biosynthetic pathways, Trends in Biotechnology, 2020, 38(7), 715-728.（并列通讯作者co-corresponding author，IF=16.6，生物工程与应用微生物一区Top期刊）
19. Song, C. #; Luan, J.#; Cui, Q.; Duan, Q.; Li, Z.; Gao, Y.; Li, R.; Li, A.; Shen, Y.; Li, Y.; Stewart, A. F.; Zhang, Y.*; Fu, J.* & Wang, H.*, Enhanced heterologous spinosad production from a 79-kb synthetic multi-operon assembly, ACS Synthetic Biology, 2019, 8(1), 137-147.（末位并列通讯作者co-corresponding author，IF=4.3，生化研究方法一区）
20. Wang, H. #; Li, Z.; Jia, R.; Yin, J.; Li, A.; Xia, L.; Yin, Y.; Muller, R.; Fu, J.*; Stewart, A. F.* & Zhang, Y.*, ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes, Nucleic Acids Research, 2018, 46(5), e28.（独立一作first author，IF=16.8，生化与分子生物学二区Top期刊）
21. Wang, H. #; Li, Z.#; Jia, R.; Hou, Y.; Yin, J.; Bian, X.; Li, A.; Muller, R.; Stewart, A. F.*; Fu, J.* & Zhang, Y.*, RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression, Nature Protocols, 2016, 11(7), 1175-1190.（首位并列一作，co-first author，IF=19.4，生化研究方法一区Top期刊）
22. Wang, H. #; Bian, X.; Xia, L.; Ding, X.; Müller, R.; Zhang, Y.*; Fu, J.* & Stewart, A. F.*, Improved seamless mutagenesis by recombineering using ccdB for counterselection, Nucleic Acids Research, 2014, 42(5), e37.（独立一作first author，IF=16.8，生化与分子生物学二区Top期刊）
23. Jing, X. #; Cui, Q. #; Li, X.; Yin, J.; Ravichandran, V.; Pan, D.; Fu, J.; Tu, Q.; Wang, H.*; Bian, X.* & Zhang, Y.*, Engineering Pseudomonas protegens Pf-5 to improve its antifungal activity and nitrogen fixation, Microbial Biotechnology, 2020, 13(1), 118-133.（并列通讯作者co-corresponding author，IF=5.9，生物工程与应用微生物二区）
24. Luan, J. #; Li, Z. #; Wang, H.*; Fu, J. & Zhang, Y., In vitro assay revealed mismatches between guide RNA and target DNA can enhance Cas9 nuclease activity, Current Chinese Science, 2020, 1(1), 69-72.（独立通讯作者corresponding author）
25. Zhang, Y. #; Liu, R. #; Tian, K. #; Wang, Z. #; Yang, X.; Gao, D.; Zhang, Y.; Fu, J.*; Wang, H.* & Zhao, J.*, Fiber2 and hexon genes are closely associated with the virulence of the emerging and highly pathogenic fowl adenovirus 4, Emerging Microbes & Infections, 2018, 7(1), 199.（并列通讯作者co-corresponding author，IF=7.2，微生物学一区Top期刊）
26. Wang, H. #; Yao, T.; Cai, M.; Xiao, X.; Ding, X. & Xia, L.*, A genome walking strategy for the identification of nucleotide sequences adjacent to known regions, Biotechnology Letters, 2013, 35(2), 279-284.（独立一作first author）
27. Sun J.; Yu, X.; Tang G.; Chen. M.; Zheng Y.; Hu Y.; Li, Q.; Li, X.; Li, N.; Li, Z.; Li, Y.; Lu, N.; Tan, W.; Yang Y.; Lyu X.; Zhao, G.; Wang, H.; Dai, L.; Zhao, G.; Ai, L.*; Zhao, W.*, A CRISPR-SpCas9M-reporting system for efficient and rapid genome editing in Caulobacter crescentus. Nucleic Acids Research, 2025. 53(8).
28. Yu, G. #; Li, X.; Duan, Q.; Fu, J.; Zhang, Y.; Wang, H. & Luan J.*, Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity. Microbial Cell Factories, 2023, 22(1): 91.
29. Hao, Y. #; Fu, J.; Zhang, J.; Du,N.; Ta, H.; Zhu, T.; Wang, H.; Lou, H.; Cheng, A.*, Identification and Functional Characterization of UDP-Glycosyltransferases Involved in Isoflavone Biosynthesis in Astragalus membranaceus. Journal of Agricultural and Food Chemistry, 2023. 71(34): p. 12775-12784.
30. Rostovskaya, M.#; Fu, J.; Obst, M.; Baer, I.; Weidlich, S.; Wang, H.; Smith, A. J. H.; Anastassiadis, K.* & Stewart, A. F.*, Transposon-mediated BAC transgenesis in human ES cells, Nucleic Acids Research, 2012, 40(19), e150.
31. Zhang, W. #; Fu, J. #; Liu, J.; Wang, H.; Schiwon, M.; Janz, S.; Schaffarczyk, L.; von der Goltz, L.; Ehrke-Schulz, E.; Domer, J.; Solanki, M.; Boehme, P.; Bergmann, T.; Lieber, A.; Lauber, C.; Dahl, A.; Petzold, A.; Zhang, Y.*; Stewart, A. F.* & Ehrhardt, A.*, An engineered virus library as a resource for the spectrum-wide exploration of virus and vector diversity, Cell Reports, 2017, 19(8), 1698-1709.
32. Zhang, W. #; Fu, J.; Liu, J.; Wang, H.; Schulz, E.; Solanki, M.; Boehme, P.; Gebbing, M.; Bergmann, T.; Doerner, J.; Stewart, A. F. & Ehrhardt, A.*, High-throughput cloning and tagging of an engineered adenovirus-library empowers vector diversity for broad applications, Molecular Therapy, 2015, 23, S21.
33. Bian, X. #; Tang, B. #; Yu, Y.; Tu, Q.; Gross, F.; Wang, H.; Li, A.; Fu, J.; Shen, Y.; Li, Y.; Stewart, A. F.; Zhao, G.; Ding, X.*; Muller, R.* & Zhang, Y.*, Heterologous production and yield improvement of epothilones in Burkholderiales strain DSM 7029, ACS Chemical Biology, 2017, 12(7), 1805-1812.
34. Yin, J. #; Wang, H.; Li, R.; Ravichandran, V.; Bian, X.; Li, A.; Tu, Q.; Stewart, A.F.; Fu, J.* & Zhang, Y.*, A practical guide to recombineering in Photorhabdus and Xenorhabdus, Current Topics in Microbiology and Immunology, 2017.
35. Ravichandran, V. #; Zhong, L.; Wang, H.; Yu, G.; Zhang, Y.* & Li, A.*, Virtual screening and biomolecular interactions of CviR-based quorum sensing inhibitors against chromobacterium violaceum, Frontiers in Cellular and Infection Microbiology, 2018, 8, 292.
36. Liu, Q. #; Shen, Q. 1; Bian, X.; Chen, H.; Fu, J.; Wang, H.; Lei, P.; Guo, Z.; Chen, W.*; Li, D.* & Zhang, Y.*, Simple and rapid direct cloning and heterologous expression of natural product biosynthetic gene cluster in Bacillus subtilis via Red/ET recombineering, Scientific Reports, 2016, 6, 34623.
37. Jiang, B. #; Zhang, R.; Feng, D.; Wang, F.; Liu, K.; Jiang, Y.; Niu, K.; Yuan, Q.; Wang, M.; Wang, H.; Zhang, Y. & Fang, X.*, A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers in Penicillium oxalicum, Frontiers in Microbiology, 2016, 7, 485.
38. Zhu, L. #; Yue, X.; Han, K.; Li, Z.; Zheng, L.; Yi, X.; Wang, H.; Zhang, Y. & Li, Y.*, Allopatric integrations selectively change host transcriptomes, leading to varied expression efficiencies of exotic genes in Myxococcus xanthus, Microbial Cell Factories, 2015, 14, 105.
39. Bian, X. #; Huang, F.; Wang, H.; Klefisch, T.; Muller, R.* & Zhang, Y.*, Heterologous production of glidobactins/luminmycins in Escherichia coli Nissle containing the glidobactin biosynthetic gene cluster from Burkholderia DSM7029, Chembiochem, 2014, 15(15), 2221-2224.
40. Yu, F. #; Jing, X.; Li, X.; Wang, H.; Chen, H.; Zhong, L.; Yin, J.; Pan, D.; Yin, Y.; Fu, J.; Xia, L.; Bian, X.; Tu, Q. & Zhang, Y., Recombineering Pseudomonas protegens CHA0: An innovative approach that improves nitrogen fixation with impressive bactericidal potency, Microbiology Research, 2019, 218, 58-65.
41. Fu, J.#; Bian, X.#; Hu, S.; Wang, H.; Huang, F.; Seibert, P. M.; Plaza, A.; Xia, L.; Müller, R.*; Stewart, A. F.* & Zhang, Y.*, Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting, Nature Biotechnology, 2012, 30(5), 440-446.