Team leader
崔球
山东大学特聘教授，博士/硕士生导师
个人简介
教育背景
博士：（2000.09-2004.3）中国科学院生物物理研究所，生物化学与分子生物学
硕士：（1997.09-2000.07）四川大学，遗传学
学士：（1993.09-1997.07）四川大学，微生物学
工作经历
2024.01-至今，山东大学微生物改造技术全国重点实验室，教授
2009.4-2023.12，中科院青岛生物能源与过程研究所，研究员
2008.01－2009.03，美国威斯康星大学麦迪逊分校，生化系，助理研究员
2004.08－2007.12，美国威斯康星大学麦迪逊分校，生化系，博士后
研究方向
1.非粮生物质的高效降解利用与秸秆糖平台
2.蛋白及脂类的先进生物制造
3.生物-化工融合工艺、自动化装备及智能优化算法
技术转移转化
1. 高效基因转化仪，青岛百奥新创生物技术有限公司
2. 高通量菌株选育系统，青岛百奥新创生物技术有限公司
3. DHA单细胞油脂生产技术，青岛琅琊台集团
4. ARA单细胞油脂生产技术，青岛琅琊台集团
5. 非粮生物质高效转化联产单细胞蛋白及油脂，山东寿光巨能金玉米开发有限公司
6. 微生物油脂高效发酵生产技术，河南新拓洋生物工程有限公司
7. 盐湖卤虫营养强化的单细胞蛋白饲料生产技术，山西南风化工集团股份有限公司
8. 微生物油脂高效生产技术，杭州合碳创物科技有限公司
获奖情况
1. 2009，中国科学院百人计划
2. 2016， 高产DHA海洋微藻的菌株选育及清洁生产示范项目，山东省科学技术奖，排名第一
3. 2017，高产ARA高产高山被孢霉的菌株选育及清洁产生示范，青岛市科技进步三等奖，排名第一
4. 2022，秸秆木质纤维清洁高效预处理关键技术及应用，中国石油和化工联合会技术发明三等奖，排名第三
5. 2023，高产DHA海洋微藻的菌株优选及清洁生产示范，中国食品工业协会科学技术奖，排名第三
6. 2024，山东省泰山产业领军人才
科研项目
1. 2025.2-2030.2，山东大学-合碳创物工程生物前沿创新研究院，企业创新联合体项目，住持
2. 2023.08-2028.07， 新一代秸秆高效糖化利用技术，山东大学微生物技术国家重点实验室揭榜挂帅项目SKLMTFCP-2023-04，主持
3. 2022.01-2025.12 木质纤维素糖化过程中的纤维小体自适应调控机制及优化，国家自然科学基金面上项目，32170051，主持
4. 2021.10-2024.01，间歇式CBS秸秆糖化中试示范，山东省能源研究院专项基金，SEIS202106，主持
5. 2020.05-2022.4，青岛生物智造GMP公共服务平台，青岛市区局会商专项，20-3-1-7-CG，主持
6. 2018.06-2020.06，纤维素乙醇关键技术示范，中科院重点培育项目，Y87230190G，主持
7. 2018.01-2020.12，重要功能脂质分子的合成途径设计及细胞工厂构建，山东省重大基础研究项目，ZR2018ZB0208，主持
8. 2018.07-2023.07，秸秆清洁预处理及一锅法糖化关键技术，中科院战略性先导科技专项（A类）子课题，XDA21060201，主持
9. 2016.06-2021.05，近海生态系统碳汇形成过程与机理，国家重点研发计划课题，2016YFA0601402，主持
10. 2015.01-2018.12，热纤梭菌纤维小体的产物抑制机理及工程改造，国家自然科学基金面上项目，31470210，主持
11. 2011.01-2015.12，细菌酶复合体纤维素降解转化机理和纤维小体的解析重构，科技部973计划课题，2011CB707404，主持
12. 2011.01-2013.12，基于超声空化及电穿孔联合作用的通用细胞基因转移系统，中科院科研装备研制项目，YZ201138，主持
代表性学术论文
2024
1.Huan Liu#*, Wei Gao#, Tianlun Cui, Sen Wang, Xiaojin Song, Zhuojun Wang, Huidan Zhang, Shiming Li, Yong-Liang Yu, Qiu Cui*(2024) A high-throughput platform enables in situ screening of fatty acid-producing strains using laser ablation electrospray ionization mass spectrometry and a Python package. Talanta 268:125234. [PMID: 37839326] [Full text (Publisher website)]
2. Fei Xiao, Tai-Ping Zhou, Sheng Dong, Tong Li, Cai-Hong Yun, Yinggang Feng, Qiu Cui, Kui Hong, Binju Wang*, Wenli Li* （2024） Molecular basis for the P450-catalyzed sp3 C‒N glycosidic bond formation in staurosporine biosynthesis. ACS Catalysis, 14, 14274–14284.
3.Sheng Dong, Jinsong Xuan, Yingang Feng, Qiu Cui (2024) Deciphering the stereo-specific catalytic mechanisms of cis-epoxysuccinate hydrolases producing L(+)-tartaric acid. Journal of Biological Chemistry 300(2)：105635-105641.
4.Yilan Wang, Yuedong Zhang, Qiu Cui, Yingang Feng, Jinsong Xuan (2024) Composition of Lignocellulose Hydrolysate in Different Biorefinery Strategies: Nutrients and Inhibitors Molecules 29(10)：2275-2301.
5.Chao Chen; Hongwu Yang; ShengDong; Cai You; Sarah Moraïs; EdwardA.Bayer; Ya-Jun Liu; Jinsong Xuan; Qiu Cui; Itzhak Mizrahi; Yingang Feng (2024) Acellulosomal double-dockerin module from Clostridium thermocellum shows distinct structural and cohesin-binding features. Protein Science. 33:e4937.
6. Min Xiao; Ya-Jun Liu; Edward A. Bayer; Akihiko Kosugi; Qiu Cui; Yingang Feng (2024) Cellulosomal hemicellulases: Indispensable players for ensuring effective lignocellulose bioconversion. Green Carbon 2(2)：57-69.
7. Sheng Dong; Chao Chen; Jie Li; Ya‐Jun Liu; Edward A. Bayer; Raphael Lamed; Itzhak Mizrahi; Qiu Cui; Yingang Feng (2024) Unique Fn3‐like biosensor in σI/anti‐σI factors for regulatory expression of major cellulosomal scaffoldins in Pseudobacteroides cellulosolvens. Protein Science, 33(11).
2023
1. Huidan Zhang, Zhuojun Wang, Caili Sun, Chuchu Zhang, Huan Liu, Qiu Cui, Xiaojin Song* and Sen Wang*（2023）A phospholipid:diacylglycerol acyltransferase is involved in the regulation of phospholipids homeostasis in oleaginous Aurantiochytrium sp. Biotechnology for Biofuels and Bioproducts16:142.[PMID:37752571] [Full text (Publisher website)]
2. 刘欢，崔球* (2023) 原位电离质谱技术在微生物菌株筛选中的应用进展，合成生物学4(5):980-999, [Full text (Publisher website)]
3. 肖艳，刘亚君，冯银刚*，崔球* 热纤梭菌在生物质能源开发中的合成生物学研究进展，合成生物学 4(XX):1-26,[Full text (Publisher website)]
4. Jie Li, Haonan Zhang, Dongyu Li, Ya-Jun Liu, Edward A. Bayer, Qi Cui, Yingang Feng*, Ping Zhu* (2023) Structure of the transcription open complex of distinct σI factors. Nature Communications 14:6455 [PMID: 37833284] [Full text (Publisher website)]
5. Chao Chen#, Bo Ma#, Yefei Wang#*, Qiu Cui, Lishan Yao, Yaohao Li, Baoquan Chen, Yingang Feng*, Zhongping Tan* (2023) Structural insight into why S-linked glycosylation cannot adequately mimic the role of natural O-glycosylation. Int. J. Biol. Macromol. 253:126649. [PMID: 37666405] [Full text (Publisher website)]
6. Huidan Zhang, Weijian Wan, Qiu Cui*, Xiaojin Song* (2023) Modular metabolic engineering of Mortierella alpina by the 2A peptide platform to improve arachidonic acid production. J. Agric. Food Chem. 71(33):12519-12527. [PMID: 37561084] [Full text (Publisher website)]
7. Yan Xiao, Sheng Dong, Ya-Jun Liu, Chun You, Yingang Feng*, Qiu Cui* (2023) Key roles of β-glucosidase BglA for the catabolism of both laminaribiose and cellobiose in the lignocellulolytic bacterium Clostridium thermocellum. Int. J. Biol. Macromol. 250:126226. [PMID: 37558019] [Full text (Publisher website)]
8. Chao Chen, Sheng Dong, Zhaoli Yu, Yichen Qiao, Jie Li, Xiaoke Ding, Renmin Li, Jinzhong Lin, Edward A. Bayer, Ya-Jun Liu, Qiu Cui, Yingang Feng* (2023) Essential autoproteolysis of bacterial anti-σ factor RsgI for transmembrane signal transduction. Sci. Adv. 9(27):eadg4846. [PMID: 37418529] [Full text (Publisher website)]
9. Ya-Jun Liu, Yuedong Zhang, Fang Chi, Chaoyang Chen, Weijian Wan, Yingang Feng, Xiaojin Song, Qiu Cui* (2023) Integrated lactic acid production from lignocellulosic agricultural wastes under thermal conditions. J. Environ. Manage. 342:118281. [PMID: 37290309] [Full text (Publisher website)]
10. Cai You, Ya-Jun Liu, Qiu Cui*, Yingang Feng* (2023) Glycoside hydrolase family 48 cellulase: A key player in cellulolytic bacteria for lignocellulose biorefinery. Fermentation 9(3):204. [Full text (Publisher website)]
11. Guang-Lei Liu, Xian-Ying Bu, Chaoyang Chen, Chunxiang Fu, Zhe Chi, Akihiko Kosugi, Qiu Cui, Zhen-Ming Chi, Ya-Jun Liu* (2023) Bioconversion of non-food corn biomass to polyol esters of fatty acid and single-cell oils. Biotechnol. Biofuels Bioprod. 16:9. [PMID: 36650607] [Full text (Publisher website)]
2022
1. Fei Yan#, Sheng Dong#, Ya-Jun Liu#, Xingzhe Yao, Chao Chen, Yan Xiao, Edward A. Bayer, Yuval Shoham, Chun You, Qiu Cui*, Yingang Feng* (2022) Deciphering cellodextrin and glucose uptake in Clostridium thermocellum. mBio 13(5):e01476-22. [PMID: 36069444] [Full text (Publisher website)]
2. 颜飞，董维亮，崔球*，刘亚君*（2022）不可降解塑料的全细胞催化降解及升级再造。生物加工过程 20(4):416-427.（综述）[Full text (Publisher website)]
3. 肖艳，王璐，王森，丛培虎，陆栋，冯银刚，崔球，宋晓金*(2022) 碳离子束诱变选育高产角鲨烯类酵母及电转化初探。南方水产科学 18(2): 98-104. [Full text (Publisher website)]
4. Chao Chen, Kuan Qi, Fang Chi, Xiaojin Song, Yingang Feng, Qiu Cui, Ya-Jun Liu* (2022) Dissolved xylan inhibits cellulosome-based saccharification by binding to the key cellulosomal component of Clostridium thermocellum. Int. J. Biol. Macromol. 207: 784-790. [PMID: 35351552] [Full text (Publisher website)]
5. 冯银刚*，刘亚君，崔球（2022）纤维小体在合成生物学中的应用研究进展。合成生物学 3(1):138-154.（综述）[Full text (Publisher website)]
2021
1. Sen Wang, Weijian Wan, Zhuojun Wang, Huidan Zhang, Huan Liu, K K I U Arunakumara, Qiu Cui, Xiaojin Song* (2021) A two-stage adaptive laboratory evolution strategy to enhance docosahexaenoic acid synthesis in oleaginous Thraustochytrid. Front. Nutr. 8:795491. [PMID: 35036411] [Full text (Publisher website)]
2. Chuanzeng Lan#, Sen Wang#, Huidan Zhang, Zhuojun Wang, Weijian Wan, Huan Liu, Yang Hu, Qiu Cui, Xiaojin Song* (2021) Cocktail biosynthesis of triacylglycerol by rational modulation of diacylglycerol acyltransferases in industrial oleaginous Aurantiochytrium. Biotechnol. Biofuels 14(1):246. [PMID: 34961557] [Full text (Publisher website)]
3. Zhuojun Wang, Sen Wang*, Yingang Feng, Weijian Wan, Huidan Zhang, Xinfeng Bai, Qiu Cui, Xiaojin Song* (2021) Obtaining high-purity docosahexaenoic acid oil in Thraustochytrid Aurantiochytrium through a combined metabolic engineering strategy. J. Agric. Food. Chem. 69(35):10215-10222. [PMID: 34415758] [Full text (Publisher website)]
4. Kuan Qi, Chao Chen, Fei Yan, Yingang Feng, Edward A. Bayer, Akihiko Kosugih, Qiu Cui, Ya-Jun Liu* (2021) Coordinated β-glucosidase activity with the cellulosome is effective for enhanced lignocellulose saccharification. Bioresour. Technol. 337: 125441. [PMID: 34182347] [Full text (Publisher website)]
5. Jie Li, Chao Chen, Ya-Jun Liu, Qiu Cui, Edward A. Bayer, Yingang Feng* (2021) NMR chemical shift assignments of a module of unknown function in the cellulosomal secondary scaffoldin ScaF from Clostridium thermocellum. Biomol. NMR Assign. 15(2):329-334. [PMID: 33876380] [Full text (Publisher website)]
6. Huidan Zhang, Qiu Cui, Xiaojin Song* (2021) Research advances on arachidonic acid production by fermentation and genetic modification of Mortierella alpina. World J. Microbiol. Biotechnol. 37(1):4. [PMID: 33392832] [Full text (Publisher website)]
7. Sheng Dong, Ya-Jun Liu, Haixia Zhou, Yan Xiao, Jian Xu, Qiu Cui*, Xinquan Wang*, Yingang Feng* (2021) Structural insight into a GH1 β-glucosidase from the oleaginous microalga, Nannochloropsis oceanica. Int. J. Biol. Macromol. 170:196-206. [PMID: 33347927] [Full text (Publisher website)]
8. Fei Yan, Ren Wei*, Qiu Cui*, Uwe T. Bornscheuer, Ya-Jun Liu* (2021) Thermophilic whole-cell degradation of polyethylene terephthalate using engineered Clostridium thermocellum. Microbial Biotechnol. 14(2):374-385. [PMID: 32343496] [Full text (Publisher website)]
2020
1. Xingzhe Yao†, Chao Chen†, Yefei Wang†, Sheng Dong, Ya-Jun Liu, Yifei Li, Zhenling Cui, Weibin Gong, Sarah Perrett, Lishan Yao, Raphael Lamed, Edward A. Bayer, Qiu Cui, Yingang Feng* (2020) Discovery and mechanism of a pH-dependent dual-binding-site switch in the interaction of a pair of protein modules. Sci. Adv. 6(43):eabd7182. [PMID: 33097546] [Full text (Publisher website)]
2. Sen Wang†, Chuanzeng Lan†, Zhuojun Wang, Weijian Wan, Huidan Zhang, Qiu Cui*, Xiaojin Song* (2020) Optimizing eicosapentaenoic acid production by grafting a heterologous polyketide synthase pathway in the Thraustochytrid Aurantiochytrium. J. Agric. Food Chem. 68(40):11253-11260. [PMID: 32829640] [Full text (Publisher website)]
3. Sen Wang†, Chuanzeng Lan†, Zhuojun Wang, Weijian Wan, Qiu Cui*, Xiaojin Song* (2020) PUFA-synthase-specific PPTase enhanced the polyunsaturated fatty acid biosynthesis via the polyketide synthase pathway in Aurantiochytrium. Biotechnol. Biofuels. 13:152.[PMID: 32874202] [Full text (Publisher website)]
4. Jie Yang, Xiaojin Song, Lina Wang*, Qiu Cui* (2020) Comprehensive analysis of metabolic alterations in Schizochytrium sp. strains with different DHA content. J. Chromatog. B 1160:122193. [PMID: 32949924] [Full text (Publisher website)]
5. Guanglei Liu, Xiaoxue Zhao, Chao Chen, Zhe Chi, Yuedong Zhang, Qiu Cui, Zhenming Chi, Ya-Jun Liu* (2020) Robust production of pigment-free pullulan from lignocellulosic hydrolysate by a new fungus co-utilizing glucose and xylose. Carbohydr. Polym. 241:116400. [PMID: 32507165] [Full text (Publisher website)]
6. Ya-Jun Liu*, Bin Li, Yingang Feng*, Qiu Cui* (2020) Consolidated bio-saccharification: Leading lignocellulose bioconversion into the real world. Biotechnol. Adv. 40:107535. [PMID: 32105675] [Full text (Publisher website)]
2019
1. Huidan Zhang, Zhuojun Wang, Yingang Feng, Qiu Cui*, Xiaojin Song* (2019) Phytohormones as stimulators to improve arachidonic acid biosynthesis in Mortierella alpine. Enzyme Microbial Technol. 131:109381. [PMID: 31615662] [Full text (Publisher website)]
2. Yanchao Wang, Xiaojin Song, Yingang Feng, Qiu Cui* (2019) Changes in peptidomes and Fischer ratios of corn-derived oligopeptides depending on enzyme hydrolysis approaches. Food Chem. 297:124931. [PMID: 31253328] [Full text (Publisher website)]
3. Zhen Wei, Chao Chen, Ya-Jun Liu, Sheng Dong, Jie Li, Kuan Qi, Shiyue Liu, Xiaoke Ding, Lizett Ortiz de Ora, Iván Muñoz-Gutiérrez, Yifei Li, Hongwei Yao, Raphael Lamed, Edward A. Bayer, Qiu Cui, Yingang Feng* (2019) Alternative σI/anti-σI factors represent a unique form of bacterial σ/anti-σ complex. Nucleic Acids Res. 47(11):5988-5997. [PMID: 31106374] [Full text (Publisher website)]
4. Guzhen Cui†, Zhuojun Wang†, Wei Hong, Ya-Jun Liu, Zhenghong Chen, Qiu Cui*, Xiaojin Song* (2019) Enhancing tricarboxylate transportation-related NADPH generation to improve biodiesel production by Aurantiochytrium. Algal Res. 40:101505. [Full text (Publisher website)]
5. Xingzhe Yao, Ya-Jun Liu, Qiu Cui, Yingang Feng* (2019) Solution structure of a unicellular microalgae-derived translationally controlled tumor protein revealed both conserved features and structural diversity. Arch. Biochem. Biophys. 665(1):23-29. [PMID: 30797749] [Full text (Publisher website)]
6. Shiyue Liu†, Ya-Jun Liu†, Yingang Feng, Bin Li and Qiu Cui* (2019) Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization. Biotechnol. Biofuels 12:35. [PMID: 30820245] [Full text (Publisher website)]
7. Chao Chen, Hongwu Yang, Jinsong Xuan, Qiu Cui, Yingang Feng* (2019) Resonance assignments of a cellulosomal double-dockerin from Clostridium thermocellum. Biomol. NMR Assign. 13(1):97-101. [PMID: 30377946] [Full text (Publisher website)]
代表性软件著作权
1．高通量基因转化系统控制平台，2023SR0808352
2．青能所代谢组学视觉预处理平台，2023SR0752423
3．青能云数据可视化分析平台，2023SR0752424
4．智能生物实验室深度学习视觉标注系统，2023SR0988017
5．智能实验室DNA序列编辑系统，2023SR0989011
6．智能液体处理系统控制平台，2023SR0808353
7．自动化生物实验室机器人集群控制平台，2023SR0808351
代表性专利
1. 采用氧气处理方法的木质纤维素全细胞糖化技术；申请号CN202210645875.1；崔球，刘亚君，陈朝阳，宋晓金
2. 原位分析微生物单菌落的激光电喷雾质谱装置；申请号202011006611.9；崔球；刘欢；宋晓金；王森；王卓君；蓝传增；2021.5.18授权
3. 原位分析微生物菌落中角鲨烯含量的方法；申请号202011006613.8；宋晓金；刘欢；崔球；王森；张慧丹；万伟建；蓝传增；2021.8.17授权
4. PET降解生物催化剂及其应用；申请号202010013024.6；崔球，刘亚君，颜飞，韦韧；2021.12.31授权
5. 富含均衡不饱和脂肪酸的橄榄油衍生物的制备及应用；申请号201910519311.1；崔球，邓煜，宋晓金，刘婷；2022.3.15授权
6. 改善鸡蛋蛋壳质量的饲料及养殖方法；申请号201910366029.4；崔球，刘冰，宋晓金，程琦，周晓峰，王彦超；2021.11.19授权
7. 一种低成本，高生物量和高蛋白量的蛋白核小球藻培养方法；申请号201811569914.4；崔球，肖艳，颜飞，祁宽；2022.4.15授权
8. 用于生产高DHA含量鸡蛋的饲料添加剂及应用；申请号201811285475.4；崔球，宋晓金，刘亚君，蓝传僧，王森
9. 用于生产适合婴幼儿的DHA-ARA鸡蛋的营养强化剂及应用；申请号201811282278.7；崔球，初欢欢，宋晓金，刘亚君，张慧丹；2021.4.13授权
10. 一种无抗生物鸡饲料的制备方法；申请号201811277537.7；崔球，刘亚君，刘世岳，李仁民
11. 生物饲料用促消化复合菌剂及其应用；发明专利号201811276681.9；崔球，初欢欢，刘亚君，刘世岳，李仁民；2020.10.20授权
12. 采用木质纤维素生产纤维寡糖的方法；发明专利号201810985861.8；崔球，刘亚君，李仁民，冯银刚；2021.1.1授权
13. 用于催化木质纤维素糖化的全菌酶制剂；申请号201810939329.2；崔球，刘亚君，刘世岳，李仁民，祁宽，冯银刚；2021.4.13授权
14. 提高木质纤维素糖化效率的全菌糖化方法；发明专利号201810939479.3；崔球，刘亚君，刘世岳，冯银刚；2020.10.20授权
15. 采用木质纤维素制备角鲨烯的方法；申请号201810939517.5；崔球，刘亚君，宋晓金；2021.4.27授权
16. 采用木质纤维素制备虾青素的方法；申请号201810939518.X；崔球，刘亚君，宋晓金；2021.4.27授权
17. 用于催化木质纤维素糖化的纤维小体酶制剂；申请号201810939532.X；崔球，刘亚君，李仁民，刘世岳，祁宽，冯银刚；2021.4.13授权
18. 采用木质纤维素培养微藻的方法；申请号201810939294.2；崔球，刘亚君，祁宽，宋晓金；2021.9.28授权
19. 采用木质纤维素生产富含多不饱和脂肪酸油脂的方法；申请号201810939296.1；崔球，刘亚君，李仁民，宋晓金，张慧丹；2022.04.15
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