姜玮，男，1962年生人，1984年7月毕业于山东大学化学系，获理学学士学位并留校任教，分别于1992年和2003年在山东大学获理学硕士、博士学位。现任山东大学化学与化工学院教授，博士生导师，院学术委员会委员，山东大学基础学部学术委员会委员，山东省化学化工学会常务理事，中国化学会会员，中国化学会有机分析专业委员会委员，山东省环境学会理事。

学习及工作经历：

1984.07 － 1991.12   山东大学化学系，助教

1991.12 － 1997.09   山东大学化学与化工学院，讲师

1997.09 － 2002.09   山东大学化学与化工学院，副教授

2002.09 － 现在         山东大学化学与化工学院，教授

2006.06 － 现在         山东大学化学与化工学院，教授，博士生导师

1999.11 － 2003.12   山东大学化学与化工学院，副院长

2004.01 － 2007.12   山东大学研究生院，副院长

2004.06 － 2007.12   兼任山东大学威海分校研究生处，处长

2007.12 － 2013.01   山东大学化学与化工学院，党委书记兼副院长

2013.01 － 2018.04   山东大学化学与化工学院，党委书记

主讲课程：

分析化学前沿，分析化学，无机及分析化学，分析化学实验，仪器分析实验

研究领域和兴趣：

研究领域为生物分析化学，研究工作集中在：
1、DNA纳米材料的设计、制备、表征与功能化；
2、多模态、多维度荧光显微成像技术与单分子检测、单细胞分析；
3、新型荧光信号放大策略与生物分子超灵敏检测技术；
4、新型纳米荧光生物传感器的设计、构建与生物医学应用。
作为项目负责人承担国家自然科学基金面上项目5项，山东省自然科学基金重点项目2项。在Analytical  Chemistry，Chemical Science, Biomaterials，Chemical Communications，Biosensor and  Bioelectronics，ACS Applied Materials & Interfaces，Bioconjugate  Chemistry等国际知名学术杂志发表SCI收录研究论文100余篇。

主要论著：

103. A bipedal-unequivalent three-dimensional DNA walker and its biosensing application

Sensors and Actuators: B. Chemical 327 (2021) 128942

102.A bimolecular i-motif mediated FRET strategy for imaging protein homodimerization on a living tumor cell surface

Chemical Communications, 2020, 56, 13405-13408

101.An interparticle relatively motional DNA walker and its sensing application

Chemical Science, 2020, 11, 7415-7423

100.A track-regenerated DNA walker: Construction and its derived sensing application

Sensors and Actuators B-Chemical, 2020, 314, 128053

99.Multifunctional molecular beacons-modified gold nanoparticles as a nanocarrier for synergistic inhibition and in situ imaging of drug-resistant-related mRNAs in living cells.

ACS Appl. Mater. Interfaces, 2019, 11, 35548-35555

98.A DNA walker powered by endogenous enzymes for imaging uracil-DNA glycosylase activity in living cells

Chemical Communications, 2019, 55, 6026-6029

97.pH-responsive ZnO nanoprobe mediated DNAzyme signal amplification strategy for sensitive detection and live cell imaging of multiple microRNAs

Sensors and Actuators B-Chemical, 2019, 293, 93-99

96.MnO₂ nanosheet-mediated ratiometric fluorescence biosensor for microRNA detection and imaging in living cells

Analytica Chimica Acta, 2019, 1063, 152-158

95.Multiple sealed primers-mediated rolling circle amplification strategy for sensitive and specific detection of DNA methyltransferase activity

Talanta, 2019, 194, 282-288.

94.T7 exonuclease-assisted and target-triggered cascade dual recycling signal amplification strategy for the sensitive and specific detection of adenosine

Talanta, 2019, 197, 234-238

93.Endogenous stimuli-responsive nucleus-targeted nanocarrier for intracellular mRNA imaging and drug delivery

ACS Appl. Mater. Interfaces 2018, 10, 39524-39531

92.Binding-induced nicking site reconstruction strategy for quantitative detection of membrane protein on living cell

Talanta, 2018, 189, 383-388

91.An enzyme-free and label-free fluorescence biosensor for microRNA detection based on cascade amplification of DNAzyme-powered three-dimensional DNA walker and hybridization chain reaction

Sensors and Actuators B-Chemical, 2018, 268, 287-292

90.Protein binding protection in combination with DNA masking for sensitive and reliable transcription factor detection

Talanta, 2018, 186, 293-298

89.A smart DNA tweezer for detection of human telomerase activity

Analytical Chemistry, 2018, 90, 3521-3530

88.An integrated and restructive probe mediated strand displacement amplification strategy for sensitive and specific DNA methyltransferase activity detection

Sensors and Actuators B-Chemical, 2018, 266, 124-130

87.Multifunctional aptamer probe mediated cascade amplification for label-free detection of adenosine

Sensors and Actuators B-Chemical, 2018, 260, 581-586

86.A dual-targeting DNA tetrahedron nanocarrier for breast cancer cell imaging and drug delivery

Talanta, 2018, 179, 356-363

85.Target binding protection mediated rolling circle amplification for sensitive detection of transcription factors

Talanta, 2018, 179, 331-336

84.Self-primer and self-template recycle rolling circle amplification strategy for sensitive detection of uracil-DNA glycosylase activity

Analytica Chimica Acta, 2018, 1001, 119-124

83.Convertible DNA ends-based silver nanoprobes for colorimetric detection human telomerase activity

Talanta, 2018, 178, 458-463

82.Integrating DNA structure switch with branched hairpins for the detection of uracil-DNA glycosylase activity and inhibitor screening

Talanta, 2018, 179, 51-56

81.Sensitive and selective detection of the p53 gene based on a triple-helix magnetic probe coupled to a fluorescent liposome hybridization assembly via rolling circle amplification

Analyst, 2017, 142, 3598-3604

80.Ultrasensitive and accurate assay of human methyltransferase activity at the single-cell level based on a single integrated magnetic microprobe

ACS Appl. Mater. Interfaces, 2017, 9, 29554-29561

79.Liposome-encoded magnetic beads initiated by padlock exponential rolling circle amplification for portable and accurate quantification of microRNAs

Chemical Communications, 2017, 53, 10772-10775

78.Uracil removal-inhibited ligase reaction in combination with catalytic hairpin assembly for sensitive and specific detection of uracil-DNA glycosylase activity

Analyst, 2017, 142, 4655-4660

77.DNA nanostructure-based drug delivery nanosystems in cancer therapy

International Journal of Pharmaceutics, 2017, 533, 169-178

76.A catalytic assembled enzyme-free three-dimensional DNA walker and its sensing application

Chemical Communications, 2017, 53, 5527-5530

75.Terminal protection-mediated autocatalytic cascade amplification coupled with graphene oxide fluorescence switch for sensitive and rapid detection of folate receptor 

Talanta, 2017, 174, 684-688

74.A target triggered proximity combination-based fluorescence sensing strategy for adenosine detection

Analyst, 2017, 142, 2247-2252

73.A loop-mediated cascade amplification strategy for highly sensitive detection of DNA methyltransferase activity

Sensors and Actuators B-Chemical, 2017, 244, 599-605

72.Sensitive detection of T4 polynucleotide kinase activity based on multifunctional magnetic probes and polymerization nicking reactions mediated hyperbranched rolling circle amplification

Biosensors and Bioelectronics, 2017, 91, 631-636

71.Label-free molecular beacons-based cascade amplification DNA machine for sensitive detection of telomerase activity

Talanta, 2017, 167, 645-650

70.Toehold-mediated strand displacement reaction-dependent fluorescent strategy for sensitive detection of uracil-DNA glycosylase activity
Biosensors and Bioelectronics, 2017, 89, 984-988

69.Colocalization recognition-activated cascade signal amplification strategy for ultrasensitive detection of transcription factors
Biosensors and Bioelectronics, 2017, 89, 978-983

68.A bicyclo-hairpin probe mediated strand displacement amplification strategy for label-free and sensitive detection of bleomycin
Sensors and Actuators B, 2017, 238, 318-324

67.Model-guided interface probe arrangement for sensitive protein detection
Analytical Chemistry, 2016, 88, 9885-9889

66.Application of an ultrahigh-performance liquid chromatography coupled to quadrupole-Orbitrap high-resolution mass spectrometry for the rapid screening, identification and quantification of illegal adulterated glucocorticoids in herbal medicines
Journal of Chromatography B, 2016, 1038, 34-42

65.Target-controlled gating liposome "off–on"cascade amplification for sensitive and accurate detection of phospholipase D in breast cancer cells with a low-background signal
Chemical Communications, 2016, 52, 10660-10663

64.Binding Induced Colocalization Activated Hybridization Chain Reaction on the Surface of Magnetic Nanobead for Sensitive Detection of Adenosine
Biosensors and Bioelectronics, 2016, 86, 966--970

63.A split recognition mode combined with cascade signal amplification strategy for highly specific, sensitive detection of microRNA
Biosensors and Bioelectronics, 2016, 86, 834-839

62.T-Hg2+-T metallo-base pair-mediated dual amplification fluorescent strategy for the selective and sensitive detection of Hg2+
RSC Advances, 2016, 6, 70984-70989

61.Protein binding-protected DNA three-way junction-mediated rolling circle amplification for sensitive and specific detection of transcription factors
RSC Advances, 2016, 6, 68846-68851

60.Modular nuclease-responsive DNA three-way junctions-based dynamic assembly of DNA device and its sensing application
Analytical Chemistry, 2016, 88, 3817-3825

59.A unique dual recognition hairpin probe mediated fluorescent amplification method for sensitive detection of uracil-DNA glycosylase and endonuclease IV activities
Analyst，2016, 141, 1789-1795

58.Au Nanoparticles Fluorescence Switch-mediated Target Recycling Amplification Strategy for Sensitive Nucleic Acid Detection
RSC Advances, 2016, 6, 10650-10654

57.Cascade Signal Amplification Based on Copper Nanoparticle-Reported Rolling Circle Amplification for Ultrasensitive Electrochemical Detection of the Prostate Cancer Biomarker
ACS Appl. Mater. Interfaces 2016, 8, 2573−2581

56.Multiplexed detection of cytokines based on dual bar-code strategy and single-molecule counting
Analytical Chemistry, 2016, 88, 1578-1584

55.Target-protected dumbbell molecular probe mediated cascade rolling circle amplification strategy for the sensitive assay of DNA methyltransferase activity
Chemical Communications, 2016, 52, 2517-2520

54.A dual amplification fluorescent strategy for sensitive detection of DNA methyltransferase activity based on strand displacement amplification and DNAzyme amplification
Biosensors and Bioelectronics, 2016, 77, 650-655

53.Label-free and enzyme-free detection of transcription factors with graphene oxide fluorescence switch-based multifunctional G-quadruplex-hairpin probe
Biosensors and Bioelectronics，2016, 75, 155-160

52.Label-free detection of nicotinamide adenine dinucleotide based on ligation-triggered exonuclease III-assisted signal amplification
RSC Advances, 2015, 5, 86625-86630

51.Label-free Molecular Beacons-Based Quadratic Isothermal exponential Amplification: A simple and Sensitive One-Pot Method to Detection DNA Methyltransferase Activity
Chemical Communications, 2015, 51, 13538-13541

50.Hairpin assembly circuit-based fluorescence cooperative amplification strategy for enzyme-free and label-free detection of small molecule
Talanta, 2015, 143: 101-106

49.FokI cleavage–inhibition strategy for the specific and accurate detection of transcription factors
Talanta, 2015,144, 44-50

48.Label-free and dual-amplified detection of protein via small molecule-ligand linked DNA and a cooperative DNA machine
Biosensors and Bioelectronics, 2015, 72, 107-113

47.Highly selective and sensitive detection of miRNA based on toehold-mediated strand displacement reaction and DNA tetrahedron substrate
Biosensors and Bioelectronics, 2015, 71, 401-406

46.Label-free fluorescence dual-amplified detection of adenosine based on exonucleaseIII-assisted DNA cycling and hybridization chain reaction
Biosensors and Bioelectronics, 2015, 70, 15-20

45.Label-free Nucleic Acid Detection Based on DNA Templated Silver Nanoclusters Fluorescent Probe
Talanta, 2015, 138, 163-168

44.A DNA machine-based fluorescence amplification strategy for sensitive detection of uracil-DNA glycosylase activity
Biosensors and Bioelectronics, 2015, 68, 654-659

43.A Binding-induced Sutured Toehold Activation for Controllable DNA Strand Displacement Reactions
Chemical Communications, 2015, 51, 2903-2906

42.Sensitive detection of transcription factors with Ag+-stabilized self-assembly triplex DNA molecular switch
Chemical Communications, 2014, 50, 14987-14990

41.Visualizing the endocytosis of phenylephrine in living cells by quantum dot-based tracking
Biomaterials, 2014, 35, 7042-7049

40.Quantitative detection of tumor necrosis factor-α by single molecule counting based on hybridization chain reaction
Biosensors and Bioelectronics, 2014, 60, 180-184

39.Toehold-mediated Strand Displacement Reaction Triggered Isothermal DNA Amplification for Highly Sensitive and Selective Fluorescent Detection of Single-base Mutation
Biosensors and Bioelectronics, 2014, 59, 276-281

38.Cascade Signal Amplification Strategy for Sensitive and Label-free DNA Detection Based on Exo III-catalyzed Recycling Coupled with Rolling Circle Amplification
Analyst, 2014, 139, 2884-2889

37.Aptamer-Based Exonuclease Protection and Enzymatic Recycling Cleavage Amplification Homogeneous Assay for Highly sensitive Detection of thrombin
Analyst, 2014, 139, 3167-3173

36.Quantitative Single-molecule Detection of Protein Based on DNA Tetrahedron Fluorescent Nanolabels
Talanta, 2014, 125, 393-399

35.An ultrasensitive fluorescence assay for protein detection by hybridization chain reaction-based DNA nanotags
Biosensors and Bioelectronics, 2014, 51, 421-425

34.Surface-enhanced Raman spectroscopy detection of polybrominated diphenylethers using a portable Raman spectrometer
Talanta, 2013, 116, 14-17

33.Cysteamine-Modified Silver Nanoparticle Aggregates for Quantitative SERS Sensing of Pentachlorophenol with a Portable Raman Spectrometer
ACS Appl. Mater. Interfaces, 2013, 5 (15), 6902-6908

32.Cysteine-Modulated Colorimetric Sensing of Extracellular Mg2+ in Rat Brain Based on the Strong Chelation Interaction between Dithiothreitol and Mg2+
Analyst, 2013,138, 3046-3052

31.A novel label-free cascade amplification strategy based on dumbbell probe-mediated rolling circle amplification-responsive G-quadruplex formation for highly sensitive and selective detection of NAD+ or ATP
Chemical Communications, 2013, 49, 2640-2642

30.A Novel Enzyme-free and Label-free Fluorescence Aptasensor for Amplified Detection of Adenosine
Biosensors and Bioelectronics, 2013, 44, 52-56

29.Selective and Sensitive Mercuric (II) Ion Detection Based on Quantum Dots and Nicking Endonuclease Assisted Signal Amplification
Biosensors and Bioelectronics, 2013, 43, 84-87

28.Electrospun porous CuO-Ag nanofibers for quantitative sensitive SERS detection
CrystEngComm, 2013, 15, 1339-1344

27.Visualization the Intracellular Location and Stability of DNA origami with a Label-free Fluorescent Probe
Chemical Communications, 2012, 48, 11301-11303

26.Quantitative Detection of Single DNA Molecules on DNA Tetrahedron Decorated Substrates
Chemical Communications, 2012, 48, 9661-9663

25.In situ fabrication of single-crystalline porous ZnO nanoplates on zinc foil to support silver nanoparticles as a stable SERS substrate
CrystEngComm, 2012, 14, 6023-6028

24.UV Photolysis-induced Generation of Graphene/M (M=Ag/Au) Hybrid Nanomaterials
Materials Letters, 2012, 84, 120-123

23.Silver nanoparticle aggregates on copper foil for reliable quantitative SERS analtsis of polycyclic aromatic hydrocarbons with a portable Raman spectrometer
Analyst, 2012, 137, 3995-4000

22.Micrococcal Nuclease Detection Based on Peptide-Bridged Energy Transfer between Quantum Dots and Dye-Labeled DNA
Talanta, 2012, 97, 533-538

21.Sensitive Detection of Proteins Using Assembled Cascade Fluorescent DNA Nanotags Based on Rolling Circle Amplication
Bioconjugate Chemistry, 2012, 23, 734-739

20.Strong Interaction between Imidazolium-Based Polycationic Polymer and Ferricyanide: toward Redox Potential Regulation for Selective In Vivo Electrochemical Measurements
Analytical Chemistry, 2012, 84, 1900-1906

19.A versatile platform for highly sensitive detection of protein: DNA enriching magnetic nanoparticles based rolling circle amplication immunoassay
Chemical Communications, 2012, 33, 3930-3932.  Inside cover paper

18.Synthesis of a quinazoline derivative: A new a 1-adrenoceptors ligand for conjugation to quantum dots to study a 1-adrenoceptors in living cells
Bioorganic & Medicinal Chemistry Letters, 2011, 21, 5905-5909

17.Label free DNA detection based on gold nanoparticles quenching fluorescence of Rhodamine B
Talanta, 2011, 85, 725-729

16.A Sensitive Enzymatic Method for Paraoxon Detection based on Enzyme Inhibition and Fluorescence Quenching
Talanta, 2011, 84, 400-405

15.Quantitative Detection of Single Molecules Using Enhancement of Dye/DNA Conjugate-Labeled Nanoparticles
Bioconjugate Chemistry, 2010, 21, 1987-1993

14.Solid phase single-molecule counting of antibody binding to supported protein layers surface with low nonspecific adsorption
Talanta, 2010, 82, 1003-1009

13.Fluorescence single-molecule counting assays for protein quantification using epi-fluorescence microscopy  with quantum dots labeling
Analytical Chimica Acta, 2010, 662, 170-176

12.DNA Quantification Based on FRET Realized by Combination with Surfactant CPB
Talanta, 2010, 81, 597-601

11.The co-luminescence effect of a europium(III)-lanthanum(III)-gatifloxacin-sodium dodecylbenzene sulfonate system and its application for the determination of trace amount of europium(III)
Journal of Luminescence, 2010, 130, 591-597

10.Quantitative detection of antibody based on single-molecule counting by total internal reflection fluorescence microscopy with quantum dot labeling
Analytica Chimica Acta, 2009, 634, 83-88

09.Rapid Determination of Minocycline in Pharmaceutical Formulations and Human Urine/Serum Samples Based on the Minocycline-Europium-Sodium Dodecylbenzene Sulfonate Luminescence System
Analytical Letters, 2009, 42, 228-242

08.Luminescence enhancement effect for the determination of balofloxacin with balofloxacin–europium (III)–sodium dodecylbenzene sulfonate system
Journal of Luminescence, 2009, 129, 90-94

07.Micelle-enhanced and terbium-sensitized spectrofluorimetric determination of gatifloxacin and its interaction mechanism
Spectrochimica Acta Part A, 2009, 72, 766-771

06.Rapid determination of gatifloxacin in biological samples and pharmaceutical products using europium-sensitized fluorescence spectrophotometry
Luminescence, 2008, 23, 7-13

05.Quantification of protein based on single-molecule counting by total internal reflection fluorescence microscopy with adsorption equilibrium
Analytica Chimica Acta, 2007, 590, 104-109

04.Fluorimetric Determination of Europium Based on the Co-fluorescence System Eu-Y-Sparfloxacin-SDS
Mikrochimica Acta, 2004, 144, 29-34

03.Determination of trace europium based on a new fluorescence system of europium-sparfloxacin-1,10-phenanthroline-sodium dodecyl sulfate
Analytical Bioanalytical Chemstry, 2003, 377(4), 681-684

02.Spectrofluorimetric determination of trace amounts terbium with 4-chlorsalicylic acid, EDTA, and cetyltrimethylammonium bromide
Analytical Sciences, 2003, 19, 923-925

01.Study of the absorption spectra of the 4f electron transitions of the praseodymium complex with ciprofloxacin and its analytical application
Analyst, 2001, 126, 1440-1443

科研项目：

01. 国家基金面上项目：内源性刺激响应的复合DNA纳米探针的构建与活细胞内功能生物分子的原位成像及调控研究（No.22074081），2021.01-2024.12，主持

02. 国家基金面上项目：基于功能化石墨烯的循环肿瘤细胞分选与活细胞原位荧光成像研究（No.21675100），2017.01-2020.12，主持

03. 国家基金面上项目：新型细胞表面膜蛋白适体荧光探针的构建与活细胞原位分析研究（No.21675101），2017.01-2020.12，主持

04. 国家基金面上项目：基于DNA催化自组装的模块化荧光生物传感技术及其生物医学应用研究 (No.21475077)，2015.01-2018.12，主持

05. 国家基金面上项目：基于结构DNA纳米材料的生物单分子荧光成像技术研究 (No.21175081)，2012.01-2015.12，主持

06. 国家基金面上项目：全内反射荧光显微术检测单个生物大分子 (No.20775043)，2008.01-2010.12，主持

07. 山东省基金重点项目：量子点结合荧光共振能量转移技术检测生物大分子 (Z2008B05)，2009.01-2011.12，主持

08. 山东省基金重点项目：单分子计数及细胞内单分子运动轨迹的观测 (Z2004B03 )，2005.01-2007.12，主持