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    姜玮

    • 教授 博士生导师 硕士生导师
    • 性别:男
    • 毕业院校:山东大学
    • 学历:博士研究生毕业
    • 学位:理学博士学位
    • 在职信息:在职
    • 所在单位:化学与化工学院
    • 入职时间: 1984-07-01

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    个人简介

    姜玮,男,1962年生人,1984年7月毕业于山东大学化学系,获理学学士学位并留校任教,分别于1992年和2003年在山东大学获理学硕士、博士学位。现任山东大学化学与化工学院教授,博士生导师,院学术委员会委员,山东大学基础学部学术委员会委员,山东省化学化工学会常务理事,中国化学会会员,中国化学会有机分析专业委员会委员,山东省环境学会理事。


    学习及工作经历:

    1984.07 - 1991.12   山东大学化学系,助教

    1991.12 - 1997.09   山东大学化学与化工学院,讲师

    1997.09 - 2002.09   山东大学化学与化工学院,副教授

    2002.09 - 现在         山东大学化学与化工学院,教授

    2006.06 - 现在         山东大学化学与化工学院,教授,博士生导师

    1999.11 - 2003.12   山东大学化学与化工学院,副院长

    2004.01 - 2007.12   山东大学研究生院,副院长

    2004.06 - 2007.12   兼任山东大学威海分校研究生处,处长

    2007.12 - 2013.01   山东大学化学与化工学院,党委书记兼副院长

    2013.01 - 2018.04   山东大学化学与化工学院,党委书记


    主讲课程:

    分析化学前沿,分析化学,无机及分析化学,分析化学实验,仪器分析实验


    研究领域和兴趣:

    研究领域为生物分析化学,研究工作集中在:
    1、DNA纳米材料的设计、制备、表征与功能化;
    2、多模态、多维度荧光显微成像技术与单分子检测、单细胞分析;
    3、新型荧光信号放大策略与生物分子超灵敏检测技术;
    4、新型纳米荧光生物传感器的设计、构建与生物医学应用。
    作为项目负责人承担国家自然科学基金面上项目5项,山东省自然科学基金重点项目2项。在Analytical  Chemistry,Chemical Science, Biomaterials,Chemical Communications,Biosensor and  Bioelectronics,ACS Applied Materials & Interfaces,Bioconjugate  Chemistry等国际知名学术杂志发表SCI收录研究论文100余篇。


    主要论著:

    103. A bipedal-unequivalent three-dimensional DNA walker and its biosensing application

    Sensors and Actuators: B. Chemical 327 (2021) 128942

    102.A bimolecular i-motif mediated FRET strategy for imaging protein homodimerization on a living tumor cell surface

    Chemical Communications, 2020, 56, 13405-13408

    101.An interparticle relatively motional DNA walker and its sensing application

    Chemical Science, 2020, 11, 7415-7423

    100.A track-regenerated DNA walker: Construction and its derived sensing application

    Sensors and Actuators B-Chemical, 2020, 314, 128053

    99.Multifunctional molecular beacons-modified gold nanoparticles as a nanocarrier for synergistic inhibition and in situ imaging of drug-resistant-related mRNAs in living cells.

    ACS Appl. Mater. Interfaces, 2019, 11, 35548-35555

    98.A DNA walker powered by endogenous enzymes for imaging uracil-DNA glycosylase activity in living cells

    Chemical Communications, 2019, 55, 6026-6029

    97.pH-responsive ZnO nanoprobe mediated DNAzyme signal amplification strategy for sensitive detection and live cell imaging of multiple microRNAs

    Sensors and Actuators B-Chemical, 2019, 293, 93-99

    96.MnO2 nanosheet-mediated ratiometric fluorescence biosensor for microRNA detection and imaging in living cells

    Analytica Chimica Acta, 2019, 1063, 152-158

    95.Multiple sealed primers-mediated rolling circle amplification strategy for sensitive and specific detection of DNA methyltransferase activity

    Talanta, 2019, 194, 282-288.

    94.T7 exonuclease-assisted and target-triggered cascade dual recycling signal amplification strategy for the sensitive and specific detection of adenosine

    Talanta, 2019, 197, 234-238

    93.Endogenous stimuli-responsive nucleus-targeted nanocarrier for intracellular mRNA imaging and drug delivery

    ACS Appl. Mater. Interfaces 2018, 10, 39524-39531

    92.Binding-induced nicking site reconstruction strategy for quantitative detection of membrane protein on living cell

    Talanta, 2018, 189, 383-388

    91.An enzyme-free and label-free fluorescence biosensor for microRNA detection based on cascade amplification of DNAzyme-powered three-dimensional DNA walker and hybridization chain reaction

    Sensors and Actuators B-Chemical, 2018, 268, 287-292

    90.Protein binding protection in combination with DNA masking for sensitive and reliable transcription factor detection

    Talanta, 2018, 186, 293-298

    89.A smart DNA tweezer for detection of human telomerase activity

    Analytical Chemistry, 2018, 90, 3521-3530

    88.An integrated and restructive probe mediated strand displacement amplification strategy for sensitive and specific DNA methyltransferase activity detection

    Sensors and Actuators B-Chemical, 2018, 266, 124-130

    87.Multifunctional aptamer probe mediated cascade amplification for label-free detection of adenosine

    Sensors and Actuators B-Chemical, 2018, 260, 581-586

    86.A dual-targeting DNA tetrahedron nanocarrier for breast cancer cell imaging and drug delivery

    Talanta, 2018, 179, 356-363

    85.Target binding protection mediated rolling circle amplification for sensitive detection of transcription factors

    Talanta, 2018, 179, 331-336

    84.Self-primer and self-template recycle rolling circle amplification strategy for sensitive detection of uracil-DNA glycosylase activity

    Analytica Chimica Acta, 2018, 1001, 119-124

    83.Convertible DNA ends-based silver nanoprobes for colorimetric detection human telomerase activity

    Talanta, 2018, 178, 458-463

    82.Integrating DNA structure switch with branched hairpins for the detection of uracil-DNA glycosylase activity and inhibitor screening

    Talanta, 2018, 179, 51-56

    81.Sensitive and selective detection of the p53 gene based on a triple-helix magnetic probe coupled to a fluorescent liposome hybridization assembly via rolling circle amplification

    Analyst, 2017, 142, 3598-3604

    80.Ultrasensitive and accurate assay of human methyltransferase activity at the single-cell level based on a single integrated magnetic microprobe

    ACS Appl. Mater. Interfaces, 2017, 9, 29554-29561

    79.Liposome-encoded magnetic beads initiated by padlock exponential rolling circle amplification for portable and accurate quantification of microRNAs

    Chemical Communications, 2017, 53, 10772-10775

    78.Uracil removal-inhibited ligase reaction in combination with catalytic hairpin assembly for sensitive and specific detection of uracil-DNA glycosylase activity

    Analyst, 2017, 142, 4655-4660

    77.DNA nanostructure-based drug delivery nanosystems in cancer therapy

    International Journal of Pharmaceutics, 2017, 533, 169-178

    76.A catalytic assembled enzyme-free three-dimensional DNA walker and its sensing application

    Chemical Communications, 2017, 53, 5527-5530

    75.Terminal protection-mediated autocatalytic cascade amplification coupled with graphene oxide fluorescence switch for sensitive and rapid detection of folate receptor 

    Talanta, 2017, 174, 684-688

    74.A target triggered proximity combination-based fluorescence sensing strategy for adenosine detection

    Analyst, 2017, 142, 2247-2252

    73.A loop-mediated cascade amplification strategy for highly sensitive detection of DNA methyltransferase activity

    Sensors and Actuators B-Chemical, 2017, 244, 599-605

    72.Sensitive detection of T4 polynucleotide kinase activity based on multifunctional magnetic probes and polymerization nicking reactions mediated hyperbranched rolling circle amplification

    Biosensors and Bioelectronics, 2017, 91, 631-636

    71.Label-free molecular beacons-based cascade amplification DNA machine for sensitive detection of telomerase activity

    Talanta, 2017, 167, 645-650

    70.Toehold-mediated strand displacement reaction-dependent fluorescent strategy for sensitive detection of uracil-DNA glycosylase activity
    Biosensors and Bioelectronics, 2017, 89, 984-988

    69.Colocalization recognition-activated cascade signal amplification strategy for ultrasensitive detection of transcription factors
    Biosensors and Bioelectronics, 2017, 89, 978-983

    68.A bicyclo-hairpin probe mediated strand displacement amplification strategy for label-free and sensitive detection of bleomycin
    Sensors and Actuators B, 2017, 238, 318-324

    67.Model-guided interface probe arrangement for sensitive protein detection
    Analytical Chemistry, 2016, 88, 9885-9889

    66.Application of an ultrahigh-performance liquid chromatography coupled to quadrupole-Orbitrap high-resolution mass spectrometry for the rapid screening, identification and quantification of illegal adulterated glucocorticoids in herbal medicines
    Journal of Chromatography B, 2016, 1038, 34-42

    65.Target-controlled gating liposome "off–on"cascade amplification for sensitive and accurate detection of phospholipase D in breast cancer cells with a low-background signal
    Chemical Communications, 2016, 52, 10660-10663

    64.Binding Induced Colocalization Activated Hybridization Chain Reaction on the Surface of Magnetic Nanobead for Sensitive Detection of Adenosine
    Biosensors and Bioelectronics, 2016, 86, 966--970

    63.A split recognition mode combined with cascade signal amplification strategy for highly specific, sensitive detection of microRNA
    Biosensors and Bioelectronics, 2016, 86, 834-839

    62.T-Hg2+-T metallo-base pair-mediated dual amplification fluorescent strategy for the selective and sensitive detection of Hg2+
    RSC Advances, 2016, 6, 70984-70989

    61.Protein binding-protected DNA three-way junction-mediated rolling circle amplification for sensitive and specific detection of transcription factors
    RSC Advances, 2016, 6, 68846-68851

    60.Modular nuclease-responsive DNA three-way junctions-based dynamic assembly of DNA device and its sensing application
    Analytical Chemistry, 2016, 88, 3817-3825

    59.A unique dual recognition hairpin probe mediated fluorescent amplification method for sensitive detection of uracil-DNA glycosylase and endonuclease IV activities
    Analyst,2016, 141, 1789-1795

    58.Au Nanoparticles Fluorescence Switch-mediated Target Recycling Amplification Strategy for Sensitive Nucleic Acid Detection
    RSC Advances, 2016, 6, 10650-10654

    57.Cascade Signal Amplification Based on Copper Nanoparticle-Reported Rolling Circle Amplification for Ultrasensitive Electrochemical Detection of the Prostate Cancer Biomarker
    ACS Appl. Mater. Interfaces 2016, 8, 2573−2581

    56.Multiplexed detection of cytokines based on dual bar-code strategy and single-molecule counting
    Analytical Chemistry, 2016, 88, 1578-1584

    55.Target-protected dumbbell molecular probe mediated cascade rolling circle amplification strategy for the sensitive assay of DNA methyltransferase activity
    Chemical Communications, 2016, 52, 2517-2520

    54.A dual amplification fluorescent strategy for sensitive detection of DNA methyltransferase activity based on strand displacement amplification and DNAzyme amplification
    Biosensors and Bioelectronics, 2016, 77, 650-655

    53.Label-free and enzyme-free detection of transcription factors with graphene oxide fluorescence switch-based multifunctional G-quadruplex-hairpin probe
    Biosensors and Bioelectronics,2016, 75, 155-160

    52.Label-free detection of nicotinamide adenine dinucleotide based on ligation-triggered exonuclease III-assisted signal amplification
    RSC Advances, 2015, 5, 86625-86630

    51.Label-free Molecular Beacons-Based Quadratic Isothermal exponential Amplification: A simple and Sensitive One-Pot Method to Detection DNA Methyltransferase Activity
    Chemical Communications, 2015, 51, 13538-13541

    50.Hairpin assembly circuit-based fluorescence cooperative amplification strategy for enzyme-free and label-free detection of small molecule
    Talanta, 2015, 143: 101-106

    49.FokI cleavage–inhibition strategy for the specific and accurate detection of transcription factors
    Talanta, 2015,144, 44-50

    48.Label-free and dual-amplified detection of protein via small molecule-ligand linked DNA and a cooperative DNA machine
    Biosensors and Bioelectronics, 2015, 72, 107-113

    47.Highly selective and sensitive detection of miRNA based on toehold-mediated strand displacement reaction and DNA tetrahedron substrate
    Biosensors and Bioelectronics, 2015, 71, 401-406

    46.Label-free fluorescence dual-amplified detection of adenosine based on exonucleaseIII-assisted DNA cycling and hybridization chain reaction
    Biosensors and Bioelectronics, 2015, 70, 15-20

    45.Label-free Nucleic Acid Detection Based on DNA Templated Silver Nanoclusters Fluorescent Probe
    Talanta, 2015, 138, 163-168

    44.A DNA machine-based fluorescence amplification strategy for sensitive detection of uracil-DNA glycosylase activity
    Biosensors and Bioelectronics, 2015, 68, 654-659

    43.A Binding-induced Sutured Toehold Activation for Controllable DNA Strand Displacement Reactions
    Chemical Communications, 2015, 51, 2903-2906

    42.Sensitive detection of transcription factors with Ag+-stabilized self-assembly triplex DNA molecular switch
    Chemical Communications, 2014, 50, 14987-14990

    41.Visualizing the endocytosis of phenylephrine in living cells by quantum dot-based tracking
    Biomaterials, 2014, 35, 7042-7049

    40.Quantitative detection of tumor necrosis factor-α by single molecule counting based on hybridization chain reaction
    Biosensors and Bioelectronics, 2014, 60, 180-184

    39.Toehold-mediated Strand Displacement Reaction Triggered Isothermal DNA Amplification for Highly Sensitive and Selective Fluorescent Detection of Single-base Mutation
    Biosensors and Bioelectronics, 2014, 59, 276-281

    38.Cascade Signal Amplification Strategy for Sensitive and Label-free DNA Detection Based on Exo III-catalyzed Recycling Coupled with Rolling Circle Amplification
    Analyst, 2014, 139, 2884-2889

    37.Aptamer-Based Exonuclease Protection and Enzymatic Recycling Cleavage Amplification Homogeneous Assay for Highly sensitive Detection of thrombin
    Analyst, 2014, 139, 3167-3173

    36.Quantitative Single-molecule Detection of Protein Based on DNA Tetrahedron Fluorescent Nanolabels
    Talanta, 2014, 125, 393-399

    35.An ultrasensitive fluorescence assay for protein detection by hybridization chain reaction-based DNA nanotags
    Biosensors and Bioelectronics, 2014, 51, 421-425

    34.Surface-enhanced Raman spectroscopy detection of polybrominated diphenylethers using a portable Raman spectrometer
    Talanta, 2013, 116, 14-17

    33.Cysteamine-Modified Silver Nanoparticle Aggregates for Quantitative SERS Sensing of Pentachlorophenol with a Portable Raman Spectrometer
    ACS Appl. Mater. Interfaces, 2013, 5 (15), 6902-6908

    32.Cysteine-Modulated Colorimetric Sensing of Extracellular Mg2+ in Rat Brain Based on the Strong Chelation Interaction between Dithiothreitol and Mg2+
    Analyst, 2013,138, 3046-3052

    31.A novel label-free cascade amplification strategy based on dumbbell probe-mediated rolling circle amplification-responsive G-quadruplex formation for highly sensitive and selective detection of NAD+ or ATP
    Chemical Communications, 2013, 49, 2640-2642

    30.A Novel Enzyme-free and Label-free Fluorescence Aptasensor for Amplified Detection of Adenosine
    Biosensors and Bioelectronics, 2013, 44, 52-56

    29.Selective and Sensitive Mercuric (II) Ion Detection Based on Quantum Dots and Nicking Endonuclease Assisted Signal Amplification
    Biosensors and Bioelectronics, 2013, 43, 84-87

    28.Electrospun porous CuO-Ag nanofibers for quantitative sensitive SERS detection
    CrystEngComm, 2013, 15, 1339-1344

    27.Visualization the Intracellular Location and Stability of DNA origami with a Label-free Fluorescent Probe
    Chemical Communications, 2012, 48, 11301-11303

    26.Quantitative Detection of Single DNA Molecules on DNA Tetrahedron Decorated Substrates
    Chemical Communications, 2012, 48, 9661-9663

    25.In situ fabrication of single-crystalline porous ZnO nanoplates on zinc foil to support silver nanoparticles as a stable SERS substrate
    CrystEngComm, 2012, 14, 6023-6028

    24.UV Photolysis-induced Generation of Graphene/M (M=Ag/Au) Hybrid Nanomaterials
    Materials Letters, 2012, 84, 120-123

    23.Silver nanoparticle aggregates on copper foil for reliable quantitative SERS analtsis of polycyclic aromatic hydrocarbons with a portable Raman spectrometer
    Analyst, 2012, 137, 3995-4000

    22.Micrococcal Nuclease Detection Based on Peptide-Bridged Energy Transfer between Quantum Dots and Dye-Labeled DNA
    Talanta, 2012, 97, 533-538

    21.Sensitive Detection of Proteins Using Assembled Cascade Fluorescent DNA Nanotags Based on Rolling Circle Amplication
    Bioconjugate Chemistry, 2012, 23, 734-739

    20.Strong Interaction between Imidazolium-Based Polycationic Polymer and Ferricyanide: toward Redox Potential Regulation for Selective In Vivo Electrochemical Measurements
    Analytical Chemistry, 2012, 84, 1900-1906

    19.A versatile platform for highly sensitive detection of protein: DNA enriching magnetic nanoparticles based rolling circle amplication immunoassay
    Chemical Communications, 2012, 33, 3930-3932.  Inside cover paper

    18.Synthesis of a quinazoline derivative: A new a 1-adrenoceptors ligand for conjugation to quantum dots to study a 1-adrenoceptors in living cells
    Bioorganic & Medicinal Chemistry Letters, 2011, 21, 5905-5909

    17.Label free DNA detection based on gold nanoparticles quenching fluorescence of Rhodamine B
    Talanta, 2011, 85, 725-729

    16.A Sensitive Enzymatic Method for Paraoxon Detection based on Enzyme Inhibition and Fluorescence Quenching
    Talanta, 2011, 84, 400-405

    15.Quantitative Detection of Single Molecules Using Enhancement of Dye/DNA Conjugate-Labeled Nanoparticles
    Bioconjugate Chemistry, 2010, 21, 1987-1993

    14.Solid phase single-molecule counting of antibody binding to supported protein layers surface with low nonspecific adsorption
    Talanta, 2010, 82, 1003-1009

    13.Fluorescence single-molecule counting assays for protein quantification using epi-fluorescence microscopy  with quantum dots labeling
    Analytical Chimica Acta, 2010, 662, 170-176

    12.DNA Quantification Based on FRET Realized by Combination with Surfactant CPB
    Talanta, 2010, 81, 597-601

    11.The co-luminescence effect of a europium(III)-lanthanum(III)-gatifloxacin-sodium dodecylbenzene sulfonate system and its application for the determination of trace amount of europium(III)
    Journal of Luminescence, 2010, 130, 591-597

    10.Quantitative detection of antibody based on single-molecule counting by total internal reflection fluorescence microscopy with quantum dot labeling
    Analytica Chimica Acta, 2009, 634, 83-88

    09.Rapid Determination of Minocycline in Pharmaceutical Formulations and Human Urine/Serum Samples Based on the Minocycline-Europium-Sodium Dodecylbenzene Sulfonate Luminescence System
    Analytical Letters, 2009, 42, 228-242

    08.Luminescence enhancement effect for the determination of balofloxacin with balofloxacin–europium (III)–sodium dodecylbenzene sulfonate system
    Journal of Luminescence, 2009, 129, 90-94

    07.Micelle-enhanced and terbium-sensitized spectrofluorimetric determination of gatifloxacin and its interaction mechanism
    Spectrochimica Acta Part A, 2009, 72, 766-771

    06.Rapid determination of gatifloxacin in biological samples and pharmaceutical products using europium-sensitized fluorescence spectrophotometry
    Luminescence, 2008, 23, 7-13

    05.Quantification of protein based on single-molecule counting by total internal reflection fluorescence microscopy with adsorption equilibrium
    Analytica Chimica Acta, 2007, 590, 104-109

    04.Fluorimetric Determination of Europium Based on the Co-fluorescence System Eu-Y-Sparfloxacin-SDS
    Mikrochimica Acta, 2004, 144, 29-34

    03.Determination of trace europium based on a new fluorescence system of europium-sparfloxacin-1,10-phenanthroline-sodium dodecyl sulfate
    Analytical Bioanalytical Chemstry, 2003, 377(4), 681-684

    02.Spectrofluorimetric determination of trace amounts terbium with 4-chlorsalicylic acid, EDTA, and cetyltrimethylammonium bromide
    Analytical Sciences, 2003, 19, 923-925

    01.Study of the absorption spectra of the 4f electron transitions of the praseodymium complex with ciprofloxacin and its analytical application
    Analyst, 2001, 126, 1440-1443


    科研项目:

    01. 国家基金面上项目:内源性刺激响应的复合DNA纳米探针的构建与活细胞内功能生物分子的原位成像及调控研究(No.22074081),2021.01-2024.12,主持

    02. 国家基金面上项目:基于功能化石墨烯的循环肿瘤细胞分选与活细胞原位荧光成像研究(No.21675100),2017.01-2020.12,主持

    03. 国家基金面上项目:新型细胞表面膜蛋白适体荧光探针的构建与活细胞原位分析研究(No.21675101),2017.01-2020.12,主持

    04. 国家基金面上项目:基于DNA催化自组装的模块化荧光生物传感技术及其生物医学应用研究 (No.21475077),2015.01-2018.12,主持

    05. 国家基金面上项目:基于结构DNA纳米材料的生物单分子荧光成像技术研究 (No.21175081),2012.01-2015.12,主持

    06. 国家基金面上项目:全内反射荧光显微术检测单个生物大分子 (No.20775043),2008.01-2010.12,主持

    07. 山东省基金重点项目:量子点结合荧光共振能量转移技术检测生物大分子 (Z2008B05),2009.01-2011.12,主持

    08. 山东省基金重点项目:单分子计数及细胞内单分子运动轨迹的观测 (Z2004B03 ),2005.01-2007.12,主持







    



    教育经历

    1980.9 -- 1984.7
    山东大学       化学       理学学士学位

    工作经历

    1984.7 -- 至今

    山东大学化学院