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The oocyte cumulus complex regulates mouse sperm migration in the oviduct.

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  • Journal:Communications Biology

  • Place of Publication:UK

  • Abstract:As the time of ovulation draws near, mouse spermatozoa move out of the isthmic reservoir, which is a prerequisite for fertilization. However, the molecular mechanism remains unclear. The present study revealed that mouse cumulus cells of oocytes-cumulus complexes (OCCs) expressed transforming growth factor-β ligand 1 (TGFB1), whereas ampullary epithelial cells expressed the TGF-β receptors, TGFBR1 and TGFBR2, and all were upregulated by luteinizing hormone (LH)/human chorionic gonadotropin (hCG). OCCs and TGFB1 increased natriuretic peptide type C (NPPC) expression in cultured ampullae via TGF-β signaling, and NPPC treatment promoted spermatozoa moving out of the isthmic reservoir of the preovulatory oviducts. Deletion of Tgfb1 in cumulus cells and Tgfbr2 in ampullary epithelial cells blocked OCC-induced NPPC expression and spermatozoa moving out of the isthmic reservoir, resulting in compromised fertilization and fertility. Oocyte-derived paracrine factors were required for promoting cumulus cell expression of TGFB1. Therefore, oocyte-dependent and cumulus cell-derived TGFB1 promotes the expression of NPPC in oviductal ampulla, which is critical for sperm migration in the oviduct and subsequent fertilization.

  • All the Authors:Hongwei Wei,Zhanying Wu,Xiaodan Zhang,Yanli Sun,Longwei Gao,Wenqing Zhang

  • First Author:Zhijuan Wang

  • Indexed by:Research Article

  • Correspondence Author:YouQiang Su,Meijia Zhang

  • Document Code:10.1038/s42003-022-04287-8

  • Volume:5

  • Issue:1

  • Page Number:1327

  • ISSN No.:2399-3642

  • Translation or Not:no

  • Date of Publication:2022-12-03

  • Date of Publication:2022-12-03

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