论文成果
LSM14B is an oocyte-specific RNA-binding protein indispensable for maternal mRNA metabolism and oocyte development in mice.
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发表刊物：
Advanced Science
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刊物所在地：
USA
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关键字：
LSM14B,RNA-binding proteins (RBPs),female infertility,mRNA metabolism,oocytes,ribonucleoprotein (RNP) complexes
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摘要：
Mammalian oogenesis features reliance on the mRNAs produced and stored during early growth phase. These are essential for producing an oocyte competent to undergo meiotic maturation and embryogenesis later when oocytes are transcriptionally silent. The fate of maternal mRNAs hence ensures the success of oogenesis and the quality of the resulting eggs. Nevertheless, how the fate of maternal mRNAs is determined remains largely elusive. RNA-binding proteins (RBPs) are crucial regulators of oogenesis, yet the identity of the full complement of RBPs expressed in oocytes is unknown. Here, a global view of oocyte-expressed RBPs is presented: mRNA-interactome capture identifies 1396 RBPs in mouse oocytes. An analysis of one of these RBPs, LSM family member 14 (LSM14B), demonstrates that this RBP is specific to oocytes and associated with many networks essential for oogenesis. Deletion of Lsm14b results in female-specific infertility and a phenotype characterized by oocytes incompetent to complete meiosis and early embryogenesis. LSM14B serves as an interaction hub for proteins and mRNAs throughout oocyte development and regulates translation of a subset of its bound mRNAs. Therefore, RNP complexes tethered by LSM14B are found exclusively in oocytes and are essential for the control of maternal mRNA fate and oocyte development.
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全部作者：
Hailian Zhao,Chunhui Yang,Ruibao Su,Min Long,Jinliang Liu,Lanying Shi
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第一作者：
Hui Li
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论文类型：
Research Article
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通讯作者：
Yuanchao Xue,You-Qiang Su
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论文编号：
10.1002/advs.202300043
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卷号：
10
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期号：
18
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页面范围：
e2300043
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ISSN号：
2198-3844
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是否译文：
否
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发表时间：
2023-04-21
下一条：MTOR-mediated interaction between the oocyte and granulosa cells regulates the development and function of both compartments in mice.
