论文成果
The oocyte cumulus complex regulates mouse sperm migration in the oviduct.
  • 发表刊物:
    Communications Biology
  • 刊物所在地:
    UK
  • 摘要:
    As the time of ovulation draws near, mouse spermatozoa move out of the isthmic reservoir, which is a prerequisite for fertilization. However, the molecular mechanism remains unclear. The present study revealed that mouse cumulus cells of oocytes-cumulus complexes (OCCs) expressed transforming growth factor-β ligand 1 (TGFB1), whereas ampullary epithelial cells expressed the TGF-β receptors, TGFBR1 and TGFBR2, and all were upregulated by luteinizing hormone (LH)/human chorionic gonadotropin (hCG). OCCs and TGFB1 increased natriuretic peptide type C (NPPC) expression in cultured ampullae via TGF-β signaling, and NPPC treatment promoted spermatozoa moving out of the isthmic reservoir of the preovulatory oviducts. Deletion of Tgfb1 in cumulus cells and Tgfbr2 in ampullary epithelial cells blocked OCC-induced NPPC expression and spermatozoa moving out of the isthmic reservoir, resulting in compromised fertilization and fertility. Oocyte-derived paracrine factors were required for promoting cumulus cell expression of TGFB1. Therefore, oocyte-dependent and cumulus cell-derived TGFB1 promotes the expression of NPPC in oviductal ampulla, which is critical for sperm migration in the oviduct and subsequent fertilization.
  • 全部作者:
    Hongwei Wei,Zhanying Wu,Xiaodan Zhang,Yanli Sun,Longwei Gao,Wenqing Zhang
  • 第一作者:
    Zhijuan Wang
  • 论文类型:
    Research Article
  • 通讯作者:
    YouQiang Su,Meijia Zhang
  • 论文编号:
    10.1038/s42003-022-04287-8
  • 卷号:
    5
  • 期号:
    1
  • 页面范围:
    1327
  • ISSN号:
    2399-3642
  • 是否译文:
  • 发表时间:
    2022-12-03

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