论文成果
The oocyte cumulus complex regulates mouse sperm migration in the oviduct.
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发表刊物：
Communications Biology
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刊物所在地：
UK
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摘要：
As the time of ovulation draws near, mouse spermatozoa move out of the isthmic reservoir, which is a prerequisite for fertilization. However, the molecular mechanism remains unclear. The present study revealed that mouse cumulus cells of oocytes-cumulus complexes (OCCs) expressed transforming growth factor-β ligand 1 (TGFB1), whereas ampullary epithelial cells expressed the TGF-β receptors, TGFBR1 and TGFBR2, and all were upregulated by luteinizing hormone (LH)/human chorionic gonadotropin (hCG). OCCs and TGFB1 increased natriuretic peptide type C (NPPC) expression in cultured ampullae via TGF-β signaling, and NPPC treatment promoted spermatozoa moving out of the isthmic reservoir of the preovulatory oviducts. Deletion of Tgfb1 in cumulus cells and Tgfbr2 in ampullary epithelial cells blocked OCC-induced NPPC expression and spermatozoa moving out of the isthmic reservoir, resulting in compromised fertilization and fertility. Oocyte-derived paracrine factors were required for promoting cumulus cell expression of TGFB1. Therefore, oocyte-dependent and cumulus cell-derived TGFB1 promotes the expression of NPPC in oviductal ampulla, which is critical for sperm migration in the oviduct and subsequent fertilization.
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全部作者：
Hongwei Wei,Zhanying Wu,Xiaodan Zhang,Yanli Sun,Longwei Gao,Wenqing Zhang
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第一作者：
Zhijuan Wang
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论文类型：
Research Article
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通讯作者：
YouQiang Su,Meijia Zhang
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论文编号：
10.1038/s42003-022-04287-8
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卷号：
5
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期号：
1
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页面范围：
1327
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ISSN号：
2399-3642
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是否译文：
否
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发表时间：
2022-12-03
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