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发表刊物：
Nature Cell Biology
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刊物所在地：
USA
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摘要：
Piwi-interacting RNAs (piRNAs) are predominantly expressed in germ cells and function in gametogenesis in various species. However, Piwi-deficient female mice are fertile and mouse oocytes express a panel of small RNAs that do not appear to be widely representative of mammals. Thus, the function of piRNAs in mammalian oogenesis remains largely unclear. Here, we generated Piwil1- and Mov10l1-deficient golden hamsters and found that all female and male mutants were sterile, with severe defects in embryogenesis and spermatogenesis, respectively. In Piwil1-deficient female hamsters, the oocytes and embryos displayed aberrant transposon accumulation and extensive transcriptomic dysregulation, and the embryos were arrested at the two-cell stage with impaired zygotic genome activation. Moreover, PIWIL1-piRNAs exert a non-redundant function in silencing endogenous retroviruses in the oocytes and embryos. Together, our findings demonstrate that piRNAs are indispensable for generating functional germ cells in golden hamsters and show the value of this model species for piRNA studies in gametogenesis, especially those related to female infertility.
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第一作者：
Hongdao Zhang
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通讯作者：
Jianmin Li,Ligang Wu,Wentao Zeng
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全部作者：
YouQiang Su,Aimin Shi,Feiyang Diao,Jianli Zhou,Wen Xiao,Heling Fu,Shiqi Gao,Aihua Zhang,Ying Zhang,Yana Lai,Li Hou,Zhaozhen Zhang,Yali Xiao,Xiaolong Lv,Mingzhe Li,Qinghua Chen,Fengjuan Zhang
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论文类型：
Research Article
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论文编号：
10.1038/s41556-021-00750-6
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卷号：
23
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期号：
9
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页面范围：
1013-1022
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ISSN号：
1476-4679
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是否译文：
否
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发表时间：
2021-09
