论文成果
The RNA-binding protein MARF1 promotes cortical neurogenesis through its RNase activity domain.
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发表刊物：
Sci Rep
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刊物所在地：
UK
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摘要：
Cortical neurogenesis is a fundamental process of brain development that is spatiotemporally regulated by both intrinsic and extrinsic cues. Although recent evidence has highlighted the significance of transcription factors in cortical neurogenesis, little is known regarding the role of RNA-binding proteins (RBPs) in the post-transcriptional regulation of cortical neurogenesis. Here, we report that meiosis arrest female 1 (MARF1) is an RBP that is expressed during neuronal differentiation. Cortical neurons expressed the somatic form of MARF1 (sMARF1) but not the oocyte form (oMARF1). sMARF1 was enriched in embryonic brains, and its expression level decreased as brain development progressed. Overexpression of sMARF1 in E12.5 neuronal progenitor cells promoted neuronal differentiation, whereas sMARF1 knockdown decreased neuronal progenitor differentiation in vitro. We also examined the function of sMARF1 in vivo using an in utero electroporation technique. Overexpression of sMARF1 increased neuronal differentiation, whereas knockdown of sMARF1 inhibited differentiation in vivo. Moreover, using an RNase domain deletion mutant of sMARF1, we showed that the RNase domain is required for the effects of sMARF1 on cortical neurogenesis in vitro. Our results further elucidate the mechanisms of post-transcriptional regulation of cortical neurogenesis by RBPs.
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全部作者：
Masashi Fujitani, Yuki Fujita, Suxiang Zhang, You-Qiang Su
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第一作者：
Yoshitaka Kanemitsu
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论文类型：
Research Article
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通讯作者：
Masashi Fujitani,Yukio Kawahara & ToshihideYamashita
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论文编号：
10.1038/s41598-017-01317-y
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卷号：
7
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期号：
1
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页面范围：
1155
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ISSN号：
2045-2322
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是否译文：
否
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发表时间：
2017-04-25
上一条：Interference with the C-terminalstructure of MARF1 causes defective oocyte meiotic division and female infertility in mice.
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