论文成果
RSPO2 Coordinates with GDF9:BMP15 Heterodimers to Promote Granulosa Cell and Oocyte Development in Mice
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所属单位：
生命科学学院
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发表刊物：
Adv Sci (Weinh).
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项目来源：
科技部/基金委
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关键字：
CTNNB1; GDFP:BMP15 heterodimer; OSF; RSPO2; SMAD2; female fertility; follicles; granulosa cells; oocytes; oocyte‐granulosa cell communication.
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摘要：
The generation of mature oocytes, a cornerstone of reproduction, relies on the coordinated interactions between oocytes and surrounding follicular somatic cells. Central to this process is the bidirectional communication between the oocyte and granulosa cells, mediated by oocyte-secreted factors (OSFs), including GDF9 and BMP15. While GDF9 and BMP15 are well-established regulators of oocyte and follicle development, the role of additional OSFs and their coordination with GDF9 and BMP15 remains largely unclear. Here, RSPO2 is identified as a key OSF that coordinates with the GDF9:BMP15 heterodimer to regulate granulosa cell development and enhance oocyte competence. RSPO2, primarily expressed in oocytes, interacts with GDF9:BMP15 to preserve transcriptomic integrity in preantral granulosa cells. This coordination is gene-specific, exhibiting either synergistic or antagonistic effects depending on the target genes, and involves crosstalk between CTNNB1- and SMAD2-dependent pathways. Conditional knockout of Rspo2 in oocytes causes severe defects in granulosa cell and oocyte development, leading to subfertility and earlier reproductive lifespan termination. Transcriptomic analysis shows that RSPO2 loss disrupts key granulosa cell genes (e.g., Amh, Ccnd2, Inhbb, Kitl) and compromises oocyte mitochondrial function, reducing developmental competence. These findings establish RSPO2 as an essential factor in the oocyte-granulosa cell regulatory loop, crucial for ovarian function and fertility.
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全部作者：
Hongjiang Li,Liji You,Shuhui Wang,Jinglei Bie,Ziyang Su,Lanying Shi
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第一作者：
Yingmei Wang
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论文类型：
基础研究
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通讯作者：
You-Qiang Su
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论文编号：
advs.202501973
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学科门类：
理学
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一级学科：
生物学
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文献类型：
J
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卷号：
e01973
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字数：
7000
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ISSN号：
Online ISSN:2198-3844
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是否译文：
否
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发表时间：
2025-06-01
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收录刊物：
SCI
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