赵广

 博士生导师  硕士生导师
教师姓名:赵广 教师拼音名称:zhaoguang 入职时间:2019-02-01 所在单位:微生物技术研究院 职务:教授 学历:研究生(博士)毕业 办公地点:青岛校区淦昌苑A座405 性别:男 学位:博士 在职信息:在职 毕业院校:南开大学 是否在职:1 学科:生物化学与分子生物学. 微生物学

论文发表

当前位置: 中文主页 >> 科研成果 >> 论文发表

Sun SM, Ding YM, Liu M, Xian M, Zhao G*. Comparison of glucose, acetate and ethanol as carbon resource for production of poly(3-hydroxybutyrate) and other acetyl-CoA derivatives. Front Bioeng Biotechnol, 2020, DOI: 10.3389/fbioe.2020.00833.

Zhou YF, Ding YM, Gao WJ, Wang JC, Liu XT, Xian M, Feng XJ*, Zhao G*. Biosynthesis of acetylacetone inspired by its biodegradation. Biotechnol Biofuels, 2020, 13:88. DOI: 10.1186/s13068-020-01725-9.

Xu Y, Zhao Z, Tong WH, Ding YM, Liu B, Shi YX, Wang JC, Sun SM, Liu M, Wang YH, Qi QS, Xian M*, Zhao G*. An acid-tolerance response system protecting exponentially growing Escherichia coli. Nat Commun, 2020, 11:1496. DOI: 10.1038/s41467-020-15350-5.

Liu M, Fu YX, Gao WJ, Xian M*, Zhao G*. Highly efficient biosynthesis of hypoxanthine in Escherichia coli and transcriptome-based analysis of the purine metabolism. ACS Synth Biol, 2020, DOI: 10.1021/acssynbio. 9b00396.

Zhao Z, Xian M, Liu M*, Zhao G*. Biochemical routes for uptake and conversion of xylose by microorganisms. Biotechnol Biofuels, 2020, 13:21. DOI:10.1186/s13068-020-1662-x

Liu HH, Zhang J, Yuan J, Jiang XL, Jiang LY, Zhao G, Huang D, Liu B. Omics-based analyses revealed metabolic responses of Clostridium acetobutylicum to lignocellulose-derived inhibitors furfural, formic acid and phenol stress for butanol fermentation. Biotechnol Biofuels, 2019, 12:101. DOI: 10.1186/s13068-019-1440-9.

Feng XJ, Gao WJ, Zhou YF, Zhao ZQ, Liu XT, Han XJ, Xian M*, Zhao G*. Coupled biosynthesis and esterification of 1,2,4-butanetriol to simplify its separation from fermentation broth. Eng Life Sci, 2019, 19:444-451.DOI: 10.1002/elsc. 201800131.

Cao YJ, Zhang RB, Liu W, Zhao G, Niu W, Guo JT, Xian M*, Liu HZ*. Manipulation of the precursor supply for high-level production of longifolene by metabolically engineered Escherichia coliSci Rep, 2019, 9:95. DOI: 10.1038/s41598-018-36495-w.

Liu B, Xiang SM, Zhao G, Wang BJ, Ma YH*, Liu WF*, Tao Y. Efficient production of 3-hydroxypropionate from fatty acids feedstock in Escherichia coliMetab Eng, 2019, 51:121. DOI: 10.1016/j.ymben.2018. 10.003.

Wang YH, Xian M, Feng XJ, Liu M*, Zhao G*. Biosynthesis of ethylene glycol from d-xylose in recombinant Escherichia coliBioengineered, 2018, 9(1): 233-241. DOI: 10.1080/21655979.2018.1478489.

Liu M, Ding YM, Xian M*, Zhao G*. Metabolic engineering of a xylose pathways for biotechnological production of glycolate in Escherichia coliMicrob Cell Fact, 2018, 17:51. DOI: 10.1186/s12934-018-0900-4.

Feng XJ, Jiang LQ, Han XJ, Liu XT, Zhao ZQ, Liu HZ, Xian M*, Zhao G*. Production of D-lactate from glucose using Klebsiella pneumoniae mutants. Microb Cell Fact, 2017, 16: 209. DOI: 10.1186/s12934-017-0822-6.

Liu H, Cheng T, Zou HB*, Zhang HB, Xu X, Sun C, Aboulnaga E, Cheng ZK, Zhao G*, Xian M*. High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coliProcess Biochem, 2017, 62: 1-9. DOI: 10.1016/j. procbio.2017.07.021.

Lactama ST Kuiate JR, Ding YM, Xian M, Liu HZ, Boudjeko T, Feng XJ*, Zhao G*. Enhanced poly(3-hydroxypropionate) production via β-alanine pathway in recombinant Escherichia coliPLoS One, 2017, 12:e0173150. DOI: 10.1371/journal.pone.0173150.

Lactama ST, Yao L, Xian M, Liu H, Kuiate JR, Liu HZ, Feng XJ*, Zhao G. A novel autolysis system controlled by magnesium and its application to poly(3-hydroxypropionate) production in engineered Escherichia coliBioengineered, 2017, 8(5):594-9. DOI: 10.1080/21655979.2017.1286432.

Liu CS, Ding YM, Xian M, Liu M, Liu HZ, Ma QJ, Zhao G*. Malonyl-CoA pathway: a promising route for 3-hydroxypropionate biosynthesis. Crit Rev Biotechnol, 2017, 37(7):933-41. DOI: 10.1080/07388551.2016. 1272093.

Cheng T, Liu H, Zou HB*, Cheng NN, Shi MX, Xie CX, Zhao G*, Xian M*. Enzymatic process optimization for the in vitro production of isoprene from mevalonate. Microb Cell Fact, 2017, 16:8. DOI: 10.1186/s12934-016-0622-4.

Liu M, Ding YM, Chen HL, Zhao Z, Liu HZ, Xian M*, Zhao G*. Improving the production of acetyl-CoA-derived chemicals in Escherichia coli BL21(DE3) through iclR and arcA deletion. BMC Microbiol, 2017, 17:10. DOI: 10.1186/s12866-016-0913-2.

Fang F, Dai BY, Zhao G, Zhao H, Sun C, Liu H*, Xian M*. In depth understanding the molecular response to the enhanced secretion of fatty acids in Sacchromyces cerevisiae due to one-step gene deletion of acyl-CoA snthetases. Process Biochem, 2016, 51(9): 1162-74. DOI: 10.1016/j.procbio.2016.05.017.

Chen DW, Liang FB, Feng DX*, Du FL, Zhao G, Liu HZ, Xian M*. Sustainable utilization of lignocellulose: Preparation of furan derivatives from carbohydrate biomass by bifunctional lignosulfonate-based catalysts. Catal Commum, 2016, 84: 159-62. DOI: 10.1016/j.catcom.2016.06.012.

Tong WH, Xu Y, Xian M, Niu W, Guo JT, Liu HZ*, Zhao G*. Biosynthetic pathway for acrylic acid from glycerol in recombinant Escherichia coliAppl Mircobiol Biotechnol, 2016, 100(11): 4901-7. DOI: 10.1007/s00253-015-7272-z.

Cao YJ, Cheng T, Zhao G, Niu W, Guo JT, Xian M*, Liu HZ*. Metabolic engineering of Escherichia coli for the production of hydroxyl fatty acids from glucose. BMC Biotechnol, 2016, 16:26. DOI: 10.1186/s12896-016-0257-x.

Liu M, Han XP, Xian M, Ding YM, Liu HZ*, Zhao G*. Development of a 3-hydroxypropionate resistant Escherichia coli strain. Bioengineered, 2016, 7(1): 21-7. DOI: 10.1080/21655979.2015.1122143.

Liu CS, Ding YM, Zhang RB, Liu HZ, Xian M*, Zhao G*. Functional balance between enzymes in malonyl-CoA pathway for 3-hydroxypropionate biosynthesis. Metab Eng, 2016, 34: 104-11. DOI: 10.1016/j.ymben.2016.01.001.

Liu M, Yao L, Xian M, Ding YM, Liu HZ*, Zhao G*. Deletion of arcA increased the production of acetyl-CoA-derived chemicals in recombinant Escherichia coliBiotechnol Lett, 2016, 38(1):97-101. DOI: 10.1007/s10529-015-1953-7.

Liu M, Feng XJ, Ding YM, Zhao G, Liu HZ*, Xian M*. Metabolic engineering of Escherichia coli to improve recombinant protein production. Appl Microbiol Biotechnol, 2015, 99(24):10367-77. DOI: 10.1007/ s00253-015-6955-9.

Feng XJ, Xian M, Liu W, Xu C, Zhang HB, Zhao G*. Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineered Klebsiella pneumoniae strain without vitamin B12. Bioengineered, 2015, 6(2):77-81. DOI: 10.1080/21655979.2015.1011027.

Feng XJ, Ding YM, Xian M, Xu X, Zhang RB, Zhao G*. Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain. Bioresour Technol, 2014, 172:269-75. DOI: 10.1016/j.biortech. 2014.09.074.

Wang Q, Yang P, Xian M, Feng L, Wang JM, Zhao G*. Metabolic engineering of Escherichia coli for poly (3-hydroxypropionate) production from glycerol and glucose. Biotechnol Lett, 2014, 36(11):2257-62. DOI: 10.1007/s10529-014-1600-8.

Gao YQ, Liu CS, Ding YM, Sun C, Zhang RB, Xian M*, Zhao G*. Development of genetically stable Escherichia coli strains for poly(3-hydroxypropionate) production. PLoS One, 2014, 9(5): e97845. DOI: 10.1371/journal.pone.0097845.

Wang Q, Yang P, Xian M, Liu H, Cao YJ, Yang Y, Zhao G*. Production of block copolymer poly(3-hydroxybutyrate)-block-poly(3-hydroxypropionate) with adjustable structure from inexpensive carbon source. ACS Macro Lett, 2013, 2(11): 996-1000. DOI: 10.1021/mz400446g.

Liu CS, Wang Q, Xian M, Ding YM, Zhao G*. Dissection of malonyl-coenzyme A reductase of Chloroflexus aurantiacus results in enzyme activity improvement. PLoS One, 2013, 8(9): e75554. DOI: 10.1371/journal.pone.0075554.

Gao YQ, Feng XJ, Xian M, Wang Q, Zhao G*. Inducible cell lysis systems in microbial production of bio-based chemicals. Appl Microbiol Biotechnol, 2013, 97(16): 7121-9. DOI: 10.1007/s00253-013-5100-x.

Wang Q, Yang P, Xian M, Yang Y, Liu CS, Xue YC, Zhao G*. Biosynthesis of Poly(3-hydroxypropionate-co-3-hydroxybutyrate) with fully controllable structures from glycerol. Bioresour Technol, 2013, 142: 741-4. DOI: 10.1016/j.biortech.2013.05.121.

Wang Q, Yang P, Liu CS, Xue YC, Xian M*, Zhao G*. Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coliBioresour Technol, 2013, 131: 548-51. DOI: 10.1016/j.biortech.2013.01.096.

Zheng YN, Li LL, Liu Q, Qin W, Yang JM, Cao YJ, Jiang XL, Zhao G, Xian M*. Boosting the free fatty acid synthesis of Escherichia coli by expression of a cytosolic Acinetobacter baylyi thioesterase. Biotechnol Biofuels, 2012, 5: 76. DOI: 10.1186/1754-6834-5-76.

Wang Q, Liu CS, Xian M, Zhang YG, Zhao G*. Biosynthetic pathway for poly(3-hydroxypropionate) in recombinant Escherichia coliJ Microbiol, 2012, 50(4): 693-7.  DOI: 10.1007/s12275-012-2234-y.

Zheng YN, Li LL, Liu Q, Yang JM, Wang X, Liu W, Xu X, Liu H, Zhao G*, Xian M*. Optimization of fatty alcohol biosynthesis pathway for selectively enhanced production of C12/14 and C16/18 fatty alcohols in engineered Escherichia coliMicrob Cell Fact, 2012, 11: 65. DOI: 10.1186/1475-2859-11-65.

Yang JM, Xian M*, Su SZ, Zhao G, Nie QJ, Jiang XL, Zheng YN, Liu W. Enhancing production of bio-isoprene using hybrid MVA pathway and isoprene synthase in E. coliPLoS One, 2012, 7(4): e33509. DOI: 10.1371/journal.pone.0033509.

Yang JM, Zhao G, Sun YZ, Zheng YN, Jiang XL, Liu W, Xian M*. Bio-isoprene production using exogenous MVA pathway and isoprene synthase in E. coliBioresour Technol, 2012, 104: 642-7. DOI: 10.1016/j.biortech.2011.10.042.

Shi Y*, Zhao G, Kong W. Genetic analysis of riboswitch-mediated transcriptional regulation responding to Mn2+ in SalmonellaJ Biol Chem, 2014, 289(16): 11353-66. DOI: 10.1074/jbc.M113.517516.

Zhao G, Kong W, Weatherspoon N, Clark-Curtiss J, Shi Y*. Mg2+ facilitates leader peptide translation to induce riboswitch-mediated transcription termination. EMBO J, 2011, 30(8): 1485-96. DOI: 10.1038/emboj.2011.66. (Contributed equally to this work)

Weatherspoon NZhao G, Kong W, Kong Y, Morigen, Andrews-Polymenis H, McClelland M, Shi Y*. The CpxR/CpxA two-component system upregulates two Tat-dependent peptidoglycan amidases to confer bacterial resistance to antimicrobial peptide protamine. J Biol Chem, 2011, 286(7): 5529-39. DOI: 10.1074/jbc.M110.200352. (Contributed equally to this work)

Zhao G, Weatherspoon N, Kong W, Curtiss R 3rd, Shi Y*. A dual-signal regulatory circuit activates transcription of a set of divergent operons in Salmonella typhimuriumP Natl Acad Sci USA, 2008, 105(52): 20924-9.  DOI: 10.1073/pnas.0807071106.

Saw JH, Mountain BW, Feng L, Omelchenko MV, Hou S, Saito JA, Stott MB, Li D, Zhao G, Wu JL, Galperin MY, Koonin EV, Makarova KS, Wolf YI, Rigden DJ, Dunfield PF, Wang L*, Alam M*. Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1. Genome Biol, 2008, 9(11): R161. DOI: 10.1186/gb-2008-9-11-r161.

Feng L, Wang W, Cheng JS, Ren Y, Zhao G, Gao CX, Tang Y, Liu XQ, Han WQ, Peng X, Liu RL and Wang L*. Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir. P Natl Acad Sci USA, 2007, 104(13): 5602-7.  DOI: 10.1073/pnas.0609650104.

Feng L, Perepelov AVZhao G, Shevelev SD, Wang Q, Senchenkova SN, Shashkov AS, Geng YQ, Reeves PR, Knirel YA and Wang L*. Structural and genetic evidence that the Escherichia coli O148 O antigen is the precursor of the Shigella dysenteriae type 1 O antigen and identification of a glucosyltransferase gene. Microbilogy, 2007, 153(1): 139-47. DOI: 10.1099/mic.0.2006/001107-0. (Contributed equally to this work)

Zhao G, Perepelov AV*, Senchenkova SN, Shashkov AS, Feng L, Li XM, Knirel YA, Wang L. Structural relation of the antigenic polysaccharides of Escherichia coli O40, Shigella dysenteriae type 9, and E. coli K47. Carbohydr Res, 2007, 342(9): 1275-9. DOI: 10.1016/j.carres.2007.03.005.

Perepelov AV*, Wang Q, Senchenkova SN, Shevelev SD, Zhao G, Shashkov AS, Feng L, Knirel YA and Wang L. Structure of a teichoic acid-like O-polysaccharide of Escherichia coli O29. Carbohydr Res, 2006, 341(12): 2176–2180. DOI: 10.1016/j.carres.2006.05.016.

Feng L, Senchenkova SN, Yang JH, Shashkov AS, Tao J, Guo HJ, Zhao G, Knirel YA, Reeves P, Wang L*. Structural and genetic characterization of the Shigella boydii type 13 O antigen. J Bacteriol, 2004, 186(2): 383-92. DOI: 10.1128/JB.186.2.383-392.2004.