Representative Publications

1.    Luan, J.^#; Song, C.; Liu, Y.; He, R.; Guo, R.; Cui, Q.; Jiang, C.; Li, X.; Hao, K.; Stewart, A. F.; Fu, J.; Zhang, Y. & Wang, H.*, Seamless site-directed mutagenesis in complex cloned DNA sequences using the RedEx method, Nature Protocols, 2024, published online.（corresponding author，IF=17.4）

2.    Yu, G. ^#; Duan, Q.; Cui, T.; Jiang, C.; Li, X.; Li, Y.; Fu, J.; Zhang, Y.;Wang, H.* & Luan, J.*, Development of a bacterial gene transcription activating strategy based on transcriptional activator positive feedback, Journal of Advanced Research, 2023, Dec 18:S2090-1232(23)00400-9.（co-corresponding author，IF=11.0）

3.    Li, X. ^#; Guo, R.; Luan, J.; Fu, J.; Zhang, Y. & Wang, H.*, Improving spinosad production by tuning expressions of the forosamine methyltransferase and the forosaminyl transferase to reduce undesired less active byproducts in the heterologous host Streptomyces albus J1074, Microbial Cell Factories, 2023, 22(1), 15.（corresponding author，IF=5.5）

4.     Jiang, C. ^#; She, Q. & Wang, H.*, Editorial: Insights in genome editing tools and mechanisms: 2022. Frontiers in Genome Editing, 2023, 5, 1240576.（corresponding author，IF=4.6）

5.   李雨桐Li, Y. ^#; 崔天琦Cui, T.; 张海林Zhang, H.; 于广乐Yu, G.; 栾霁Luan, J.*& 王海龙Wang, H.*，肿瘤靶向细菌Escherichia coli Nissle 1917在癌症治疗中的研究进展Research progress of tumor-targeting bacteria Escherichia coli Nissle 1917 in cancer treatment，中国生物工程杂志China Biotechnology，2023, 43(6): 54-68.（co-corresponding author，in Chinese）

6.   姜婵娟Jiang, C. ^#; 崔天琦Cui, T.; 孙洪娈Sun, H.; 焦念志Jiao, N.; 符军Fu, J.; 张友明Zhang, Y.*; 王海龙Wang, H.*，ExoCET-BAC策略高效抓取和组装高AT含量基因组大片段Efficient capture and assembly of AT-rich genomic fragments using ExoCET-BAC strategy，合成生物学Synthetic Biology Journal，2022, 3(1): 238-251.（co-corresponding author, in Chinese）Award：Outstanding Papers of 2024 in Synthetic Biology Journal

7.    Jiang, T. ^#,*; Yang, X. ^#; Li, G.; Zhao, X.; Sun, T.; Muller, R.; Wang, H.*; Li, M.* & Zhang, Y.*, Bacteria-based live vehicle for in vivo bioluminescence imaging, Analytical Chemistry, 2021, 93(47), 15687-15695.（co-corresponding author，IF=6.5）

8.    Tang, Y. ^#; Zhang, C.; Cui, T.; Lei, P.; Guo, Z. *; Wang, H.*& Liu, Q.*, The discovery of actinospene, a new polyene macrolide with broad activity against plant fungal pathogens and pathogenic yeasts, Molecules, 2021, 26(22).（co-corresponding author，IF=4.6）

9.     Song, C.^#; Luan, J.^#; Li, R.; Jiang, C.; Hou, Y.; Cui, Q.; Cui, T.; Tan, L.; Ma, Z.; Tang, Y. J.; Stewart, A. F.; Fu, J.; Zhang, Y. & Wang, H.*, RedEx: a method for seamless DNA insertion and deletion in large multimodular polyketide synthase gene clusters, Nucleic Acids Research, 2020, 48(22), e130.（corresponding author，IF=16.1）

10.     Jiang, C. ^#; Zhou, H; Sun, H; He, R; Song, C; Cui, T; Luan, J; Fu, J; Zhang, Y; Jiao, N; Wang, H.*, Establishing an efficient salinomycin biosynthetic pathway in three heterologous Streptomyces hosts by constructing a 106-kb multioperon artificial gene cluster, Biotechnology & Bioengineering, 2021, 118: 4668-4677.（corresponding author，IF=4.0）

11.    Abbasi, M. N. ^#; Fu, J.; Bian, X.; Wang, H.*; Zhang, Y.* & Li, A.*, Recombineering for genetic engineering of natural product biosynthetic pathways, Trends in Biotechnology, 2020, 38(7), 715-728.（co-corresponding author，IF=16.8）

12.    Song, C. ^#; Luan, J.^#; Cui, Q.; Duan, Q.; Li, Z.; Gao, Y.; Li, R.; Li, A.; Shen, Y.; Li, Y.; Stewart, A. F.; Zhang, Y.*; Fu, J.* & Wang, H.*, Enhanced heterologous spinosad production from a 79-kb synthetic multi-operon assembly, ACS Synthetic Biology, 2019, 8(1), 137-147.（co-corresponding author，IF=4.2）

13.    Wang, H. ^#; Li, Z.; Jia, R.; Yin, J.; Li, A.; Xia, L.; Yin, Y.; Muller, R.; Fu, J.*; Stewart, A. F.* & Zhang, Y.*, ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes, Nucleic Acids Research, 2018, 46(5), e28.（first author，IF=16.1）

14.    Wang, H. ^#; Li, Z.^#; Jia, R.; Hou, Y.; Yin, J.; Bian, X.; Li, A.; Muller, R.; Stewart, A. F.*; Fu, J.* & Zhang, Y.*, RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression, Nature Protocols, 2016, 11(7), 1175-1190.（co-first author，IF=17.4）

15.    Wang, H. ^#; Bian, X.; Xia, L.; Ding, X.; Müller, R.; Zhang, Y.*; Fu, J.* & Stewart, A. F.*, Improved seamless mutagenesis by recombineering using ccdB for counterselection, Nucleic Acids Research, 2014, 42(5), e37.（first author，IF=16.1）

16.    Jing, X. ^#; Cui, Q. ^#; Li, X.; Yin, J.; Ravichandran, V.; Pan, D.; Fu, J.; Tu, Q.; Wang, H.*; Bian, X.* & Zhang, Y.*, Engineering Pseudomonas protegens Pf-5 to improve its antifungal activity and nitrogen fixation, Microbial Biotechnology, 2020, 13(1), 118-133.（co-corresponding author，IF=5.8）

17.    Luan, J. ^#; Li, Z. ^#; Wang, H.*; Fu, J. & Zhang, Y., In vitro assay revealed mismatches between guide RNA and target DNA can enhance Cas9 nuclease activity, Current Chinese Science, 2020, 1(1), 69-72.（corresponding author）

18.    Zhang, Y. ^#; Liu, R. ^#; Tian, K. ^#; Wang, Z. ^#; Yang, X.; Gao, D.; Zhang, Y.; Fu, J.*; Wang, H.* & Zhao, J.*, Fiber2 and hexon genes are closely associated with the virulence of the emerging and highly pathogenic fowl adenovirus 4, Emerging Microbes & Infections, 2018, 7(1), 199.（co-corresponding author，IF=8.3）

19.    Wang, H. ^#; Yao, T.; Cai, M.; Xiao, X.; Ding, X. & Xia, L.*, A genome walking strategy for the identification of nucleotide sequences adjacent to known regions, Biotechnology Letters, 2013, 35(2), 279-284.（first author）

20.     Yu, G. ^#; Li, X.; Duan, Q.; Fu, J.; Zhang, Y.; Wang, H. & Luan J.*, Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity. Microbial Cell Factories, 2023, 22(1): 91.

21.    Rostovskaya, M. ^#; Fu, J.; Obst, M.; Baer, I.; Weidlich, S.; Wang, H.; Smith, A. J. H.; Anastassiadis, K.* & Stewart, A. F.*, Transposon-mediated BAC transgenesis in human ES cells, Nucleic Acids Research, 2012, 40(19), e150.

22.    Zhang, W. ^#; Fu, J. ^#; Liu, J.; Wang, H.; Schiwon, M.; Janz, S.; Schaffarczyk, L.; von der Goltz, L.; Ehrke-Schulz, E.; Domer, J.; Solanki, M.; Boehme, P.; Bergmann, T.; Lieber, A.; Lauber, C.; Dahl, A.; Petzold, A.; Zhang, Y.*; Stewart, A. F.* & Ehrhardt, A.*, An engineered virus library as a resource for the spectrum-wide exploration of virus and vector diversity, Cell Reports, 2017, 19(8), 1698-1709.

23.    Zhang, W. ^#; Fu, J.; Liu, J.; Wang, H.; Schulz, E.; Solanki, M.; Boehme, P.; Gebbing, M.; Bergmann, T.; Doerner, J.; Stewart, A. F. & Ehrhardt, A.*, High-throughput cloning and tagging of an engineered adenovirus-library empowers vector diversity for broad applications, Molecular Therapy, 2015, 23, S21.

24.    Bian, X. ^#; Tang, B. ^#; Yu, Y.; Tu, Q.; Gross, F.; Wang, H.; Li, A.; Fu, J.; Shen, Y.; Li, Y.; Stewart, A. F.; Zhao, G.; Ding, X.*; Muller, R.* & Zhang, Y.*, Heterologous production and yield improvement of epothilones in Burkholderiales strain DSM 7029, ACS Chemical Biology, 2017, 12(7), 1805-1812.

25.    Yin, J. ^#; Wang, H.; Li, R.; Ravichandran, V.; Bian, X.; Li, A.; Tu, Q.; Stewart, A.F.; Fu, J.* & Zhang, Y.*, A practical guide to recombineering in Photorhabdus and Xenorhabdus, Current Topics in Microbiology and Immunology, 2017.

26.    Ravichandran, V. ^#; Zhong, L.; Wang, H.; Yu, G.; Zhang, Y.* & Li, A.*, Virtual screening and biomolecular interactions of CviR-based quorum sensing inhibitors against chromobacterium violaceum, Frontiers in Cellular and Infection Microbiology, 2018, 8, 292.

27.    Liu, Q. ^#; Shen, Q. ¹; Bian, X.; Chen, H.; Fu, J.; Wang, H.; Lei, P.; Guo, Z.; Chen, W.*; Li, D.* & Zhang, Y.*, Simple and rapid direct cloning and heterologous expression of natural product biosynthetic gene cluster in Bacillus subtilis via Red/ET recombineering, Scientific Reports, 2016, 6, 34623.

28.    Jiang, B. ^#; Zhang, R.; Feng, D.; Wang, F.; Liu, K.; Jiang, Y.; Niu, K.; Yuan, Q.; Wang, M.; Wang, H.; Zhang, Y. & Fang, X.*, A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers in Penicillium oxalicum, Frontiers in Microbiology, 2016, 7, 485.

29.    Zhu, L. ^#; Yue, X.; Han, K.; Li, Z.; Zheng, L.; Yi, X.; Wang, H.; Zhang, Y. & Li, Y.*, Allopatric integrations selectively change host transcriptomes, leading to varied expression efficiencies of exotic genes in Myxococcus xanthus, Microbial Cell Factories, 2015, 14, 105.

30.    Bian, X. ^#; Huang, F.; Wang, H.; Klefisch, T.; Muller, R.* & Zhang, Y.*, Heterologous production of glidobactins/luminmycins in Escherichia coli Nissle containing the glidobactin biosynthetic gene cluster from Burkholderia DSM7029, Chembiochem, 2014, 15(15), 2221-2224.

31.    Yu, F. ^#; Jing, X.; Li, X.; Wang, H.; Chen, H.; Zhong, L.; Yin, J.; Pan, D.; Yin, Y.; Fu, J.; Xia, L.; Bian, X.; Tu, Q. & Zhang, Y., Recombineering Pseudomonas protegens CHA0: An innovative approach that improves nitrogen fixation with impressive bactericidal potency, Microbiology Research, 2019, 218, 58-65.

32.    Fu, J.^#; Bian, X.^#; Hu, S.; Wang, H.; Huang, F.; Seibert, P. M.; Plaza, A.; Xia, L.; Müller, R.*; Stewart, A. F.* & Zhang, Y.*, Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting, Nature Biotechnology, 2012, 30(5), 440-446.