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赵广

        2002年和2007年在南开大学获得微生物学专业学士和博士学位,2007-2011年在亚利桑那州立大学进行博士后研究,2011年加入中国科学院青岛生物能源与过程研究所,2020年调入山东大学微生物技术研究院。2012年获中国科学院“百人计划”支持,2017年获国家自然科学基金委员会“优秀青年科学基金”和“山东省自然科学杰出青年基金”资助。已在Nat Commun、Nucleic Acids Res、Metab Eng、PNAS、Bioresour Technol等国际主流刊物上发表SCI论文60余篇,参与撰写英文书籍章节2章,申请发明专利30余项,其中18项已获得授权。


发表论文:(*通讯作者,†并列第一作者)

Liu M, Guo LK, Fu YX, Huo MT, Qi QS, Zhao G*. Bacterial protein acetylation and its role in cellular physiology and metabolic regulation. Biotechnol Adv, 2021, 53: 107842. DOI: 10.1016/j.biotechadv.2021. 107842.

Wang JC, Sui XY, Ding YM, Fu YX, Feng XJ, Liu M, Zhang YM, Xian M*, Zhao G*. A fast and robust iterative genome-editing method based on a rock-paper-scissors strategy. Nucleic Acids Res, 2020, DOI: 10.1093/nar/gkaa1141.

Liu XT, Feng XJ, Ding YM, Gao WJ, Xian M, Wang JC*, Zhao G*. Characterization and directed evolution of propionyl-CoA carboxylase and its application in succinate biosynthetic pathway with two CO2 fixation reactions. Metab Eng, 2020, 62:42-50. DOI: 10.1016/j.ymben.2020.08.012.

Sun SM, Ding YM, Liu M, Xian M, Zhao G*. Comparison of glucose, acetate and ethanol as carbon resource for production of poly(3-hydroxybutyrate) and other acetyl-CoA derivatives. Front Bioeng Biotechnol, 2020, 8:33. DOI: 10.3389/fbioe.2020.00833.

Zhou YF, Ding YM, Gao WJ, Wang JC, Liu XT, Xian M, Feng XJ*, Zhao G*. Biosynthesis of acetylacetone inspired by its biodegradation. Biotechnol Biofuels, 2020, 13:88. DOI: 10.1186/s13068-020-01725-9.

Xu Y, Zhao Z, Tong WH, Ding YM, Liu B, Shi YX, Wang JC, Sun SM, Liu M, Wang YH, Qi QS, Xian M*, Zhao G*. An acid-tolerance response system protecting exponentially growing Escherichia coli. Nat Commun, 2020, 11:1496. DOI: 10.1038/s41467-020-15350-5.

Liu M, Fu YX, Gao WJ, Xian M*, Zhao G*. Highly efficient biosynthesis of hypoxanthine in Escherichia coli and transcriptome-based analysis of the purine metabolism. ACS Synth Biol, 2020, 9(3):525-535. DOI: 10.1021/acssynbio. 9b00396.

Zhao Z, Xian M, Liu M*, Zhao G*. Biochemical routes for uptake and conversion of xylose by microorganisms. Biotechnol Biofuels, 2020, 13:21. DOI:10.1186/s13068-020-1662-x

Feng XJ, Gao WJ, Zhou YF, Zhao ZQ, Liu XT, Han XJ, Xian M*, Zhao G*. Coupled biosynthesis and esterification of 1,2,4-butanetriol to simplify its separation from fermentation broth. Eng Life Sci, 2019, 19:444-451.DOI: 10.1002/elsc. 201800131.

Liu B, Xiang SM, Zhao G, Wang BJ, Ma YH*, Liu WF*, Tao Y. Efficient production of 3-hydroxypropionate from fatty acids feedstock in Escherichia coliMetab Eng, 2019, 51:121. DOI: 10.1016/j.ymben.2018. 10.003.

Wang YH, Xian M, Feng XJ, Liu M*, Zhao G*. Biosynthesis of ethylene glycol from d-xylose in recombinant Escherichia coliBioengineered, 2018, 9(1): 233-241. DOI: 10.1080/21655979.2018.1478489.

Liu M, Ding YM, Xian M*, Zhao G*. Metabolic engineering of a xylose pathways for biotechnological production of glycolate in Escherichia coliMicrob Cell Fact, 2018, 17:51. DOI: 10.1186/s12934-018-0900-4.

Feng XJ, Jiang LQ, Han XJ, Liu XT, Zhao ZQ, Liu HZ, Xian M*, Zhao G*. Production of D-lactate from glucose using Klebsiella pneumoniae mutants. Microb Cell Fact, 2017, 16: 209. DOI: 10.1186/s12934-017-0822-6.

Liu H, Cheng T, Zou HB*, Zhang HB, Xu X, Sun C, Aboulnaga E, Cheng ZK, Zhao G*, Xian M*. High titer mevalonate fermentation and its feeding as a building block for isoprenoids (isoprene and sabinene) production in engineered Escherichia coliProcess Biochem, 2017, 62: 1-9. DOI: 10.1016/j. procbio.2017.07.021.

Lactama ST Kuiate JR, Ding YM, Xian M, Liu HZ, Boudjeko T, Feng XJ*, Zhao G*. Enhanced poly(3-hydroxypropionate) production via β-alanine pathway in recombinant Escherichia coliPLoS One, 2017, 12:e0173150. DOI: 10.1371/journal.pone.0173150.

Lactama ST, Yao L, Xian M, Liu H, Kuiate JR, Liu HZ, Feng XJ*, Zhao G. A novel autolysis system controlled by magnesium and its application to poly(3-hydroxypropionate) production in engineered Escherichia coliBioengineered, 2017, 8(5):594-9. DOI: 10.1080/21655979.2017.1286432.

Liu CS, Ding YM, Xian M, Liu M, Liu HZ, Ma QJ, Zhao G*. Malonyl-CoA pathway: a promising route for 3-hydroxypropionate biosynthesis. Crit Rev Biotechnol, 2017, 37(7):933-41. DOI: 10.1080/07388551.2016. 1272093.

Cheng T, Liu H, Zou HB*, Cheng NN, Shi MX, Xie CX, Zhao G*, Xian M*. Enzymatic process optimization for the in vitro production of isoprene from mevalonate. Microb Cell Fact, 2017, 16:8. DOI: 10.1186/s12934-016-0622-4.

Liu M, Ding YM, Chen HL, Zhao Z, Liu HZ, Xian M*, Zhao G*. Improving the production of acetyl-CoA-derived chemicals in Escherichia coli BL21(DE3) through iclR and arcA deletion. BMC Microbiol, 2017, 17:10. DOI: 10.1186/s12866-016-0913-2.

Tong WH, Xu Y, Xian M, Niu W, Guo JT, Liu HZ*, Zhao G*. Biosynthetic pathway for acrylic acid from glycerol in recombinant Escherichia coliAppl Mircobiol Biotechnol, 2016, 100(11): 4901-7. DOI: 10.1007/s00253-015-7272-z.

Liu M, Han XP, Xian M, Ding YM, Liu HZ*, Zhao G*. Development of a 3-hydroxypropionate resistant Escherichia coli strain. Bioengineered, 2016, 7(1): 21-7. DOI: 10.1080/21655979.2015.1122143.

Liu CS, Ding YM, Zhang RB, Liu HZ, Xian M*, Zhao G*. Functional balance between enzymes in malonyl-CoA pathway for 3-hydroxypropionate biosynthesis. Metab Eng, 2016, 34: 104-11. DOI: 10.1016/j.ymben.2016.01.001.

Liu M, Yao L, Xian M, Ding YM, Liu HZ*, Zhao G*. Deletion of arcA increased the production of acetyl-CoA-derived chemicals in recombinant Escherichia coliBiotechnol Lett, 2016, 38(1):97-101. DOI: 10.1007/s10529-015-1953-7.

Liu M, Feng XJ, Ding YM, Zhao G, Liu HZ*, Xian M*. Metabolic engineering of Escherichia coli to improve recombinant protein production. Appl Microbiol Biotechnol, 2015, 99(24):10367-77. DOI: 10.1007/ s00253-015-6955-9.

Feng XJ, Xian M, Liu W, Xu C, Zhang HB, Zhao G*. Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineered Klebsiella pneumoniae strain without vitamin B12. Bioengineered, 2015, 6(2):77-81. DOI: 10.1080/21655979.2015.1011027.

Feng XJ, Ding YM, Xian M, Xu X, Zhang RB, Zhao G*. Production of optically pure D-lactate from glycerol by engineered Klebsiella pneumoniae strain. Bioresour Technol, 2014, 172:269-75. DOI: 10.1016/j.biortech. 2014.09.074.

Wang Q, Yang P, Xian M, Feng L, Wang JM, Zhao G*. Metabolic engineering of Escherichia coli for poly (3-hydroxypropionate) production from glycerol and glucose. Biotechnol Lett, 2014, 36(11):2257-62. DOI: 10.1007/s10529-014-1600-8.

Gao YQ, Liu CS, Ding YM, Sun C, Zhang RB, Xian M*, Zhao G*. Development of genetically stable Escherichia coli strains for poly(3-hydroxypropionate) production. PLoS One, 2014, 9(5): e97845. DOI: 10.1371/journal.pone.0097845.

Wang Q, Yang P, Xian M, Liu H, Cao YJ, Yang Y, Zhao G*. Production of block copolymer poly(3-hydroxybutyrate)-block-poly(3-hydroxypropionate) with adjustable structure from inexpensive carbon source. ACS Macro Lett, 2013, 2(11): 996-1000. DOI: 10.1021/mz400446g.

Liu CS, Wang Q, Xian M, Ding YM, Zhao G*. Dissection of malonyl-coenzyme A reductase of Chloroflexus aurantiacus results in enzyme activity improvement. PLoS One, 2013, 8(9): e75554. DOI: 10.1371/journal.pone.0075554.

Gao YQ, Feng XJ, Xian M, Wang Q, Zhao G*. Inducible cell lysis systems in microbial production of bio-based chemicals. Appl Microbiol Biotechnol, 2013, 97(16): 7121-9. DOI: 10.1007/s00253-013-5100-x.

Wang Q, Yang P, Xian M, Yang Y, Liu CS, Xue YC, Zhao G*. Biosynthesis of Poly(3-hydroxypropionate-co-3-hydroxybutyrate) with fully controllable structures from glycerol. Bioresour Technol, 2013, 142: 741-4. DOI: 10.1016/j.biortech.2013.05.121.

Wang Q, Yang P, Liu CS, Xue YC, Xian M*, Zhao G*. Biosynthesis of poly(3-hydroxypropionate) from glycerol by recombinant Escherichia coliBioresour Technol, 2013, 131: 548-51. DOI: 10.1016/j.biortech.2013.01.096.

Wang Q, Liu CS, Xian M, Zhang YG, Zhao G*. Biosynthetic pathway for poly(3-hydroxypropionate) in recombinant Escherichia coliJ Microbiol, 2012, 50(4): 693-7.  DOI: 10.1007/s12275-012-2234-y.

Zheng YN, Li LL, Liu Q, Yang JM, Wang X, Liu W, Xu X, Liu H, Zhao G*, Xian M*. Optimization of fatty alcohol biosynthesis pathway for selectively enhanced production of C12/14 and C16/18 fatty alcohols in engineered Escherichia coliMicrob Cell Fact, 2012, 11: 65. DOI: 10.1186/1475-2859-11-65.

Shi Y*, Zhao G, Kong W. Genetic analysis of riboswitch-mediated transcriptional regulation responding to Mn2+ in SalmonellaJ Biol Chem, 2014, 289(16): 11353-66. DOI: 10.1074/jbc.M113.517516.

Zhao G, Kong W, Weatherspoon N, Clark-Curtiss J, Shi Y*. Mg2+ facilitates leader peptide translation to induce riboswitch-mediated transcription termination. EMBO J, 2011, 30(8): 1485-96. DOI: 10.1038/emboj.2011.66

Weatherspoon NZhao G, Kong W, Kong Y, Morigen, Andrews-Polymenis H, McClelland M, Shi Y*. The CpxR/CpxA two-component system upregulates two Tat-dependent peptidoglycan amidases to confer bacterial resistance to antimicrobial peptide protamine. J Biol Chem, 2011, 286(7): 5529-39. DOI: 10.1074/jbc.M110.200352

Zhao G, Weatherspoon N, Kong W, Curtiss R 3rd, Shi Y*. A dual-signal regulatory circuit activates transcription of a set of divergent operons in Salmonella typhimuriumP Natl Acad Sci USA, 2008, 105(52): 20924-9.  DOI: 10.1073/pnas.0807071106.

Feng L, Wang W, Cheng JS, Ren Y, Zhao G, Gao CX, Tang Y, Liu XQ, Han WQ, Peng X, Liu RL and Wang L*. Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir. P Natl Acad Sci USA, 2007, 104(13): 5602-7.  DOI: 10.1073/pnas.0609650104.

Feng L, Perepelov AVZhao G, Shevelev SD, Wang Q, Senchenkova SN, Shashkov AS, Geng YQ, Reeves PR, Knirel YA and Wang L*. Structural and genetic evidence that the Escherichia coli O148 O antigen is the precursor of the Shigella dysenteriae type 1 O antigen and identification of a glucosyltransferase gene. Microbilogy, 2007, 153(1): 139-47. DOI: 10.1099/mic.0.2006/001107-0

Zhao G, Perepelov AV*, Senchenkova SN, Shashkov AS, Feng L, Li XM, Knirel YA, Wang L. Structural relation of the antigenic polysaccharides of Escherichia coli O40, Shigella dysenteriae type 9, and E. coli K47. Carbohydr Res, 2007, 342(9): 1275-9. DOI: 10.1016/j.carres.2007.03.005.