李文斐
Professor Supervisor of Doctorate Candidates Supervisor of Master's Candidates
Name (Simplified Chinese):李文斐
E-Mail:
Date of Employment:2021-02
School/Department:Shandong University, School of Basic Medical Sciences
Administrative Position:Professor
Education Level:Postgraduate (Postdoctoral)
Business Address:School of Basic Medical Sciences， Shandong University
Gender:Female
Contact Information:
Degree:Doctor
Academic Titles:医学结构生物学中心副主任
Alma Mater:Tsinghua University
College:School of Basic Medical Sciences
Discipline:Biochemistry and Molecular Biology
Cell Biology
Biophysics
Genetics
Honor
2022 BMS本科生优秀班主任
2022 泰山学者青年专家
2021 齐鲁青年学者（第一层次）
2022 山东省优秀青年（海外）
2015 北京市优秀毕业生
2009 北京市大学生科学研究与创业
2015 博士生毕业论文奖
国家奖学金
2013 清华大学学生实验室建设贡献二等奖
Hits:
Title of Paper:Human ribosome interactions reframe neomycin toxicity
Journal:bioRxiv 预印本ing
Summary:Aminoglycosides like neomycin are widely used but clinically limited due to toxicity, traditionally attributed to mitoribosome inhibition. Here, we demonstrate that this assumption requires re-evaluation by comparing neomycin’s interactions with human ribosomes in vitro and in cells. While cryo-EM and biochemical assays reveal strong in vitro binding to both mitoribosomes and cytosolic ribosomes, especially at conserved regions such as the helix 44 (h44) decoding center despite sequence divergence, and H69. Cellular analyses show minimal impact on global translation, reduced occupancy on cytosolic ribosomes, and a complete absence of neomycin on mitoribosomes. These discrepancies suggest limited mitochondrial permeability rather than direct mitoribosome inhibition underlies neomycin’s toxicity. Our findings redefine the mechanistic basis of aminoglycosides side effects and call for a reassessment of their cellular targets.
Translation or Not:No
Date of Publication:2025-08
Links to Published Journals:https://www.biorxiv.org/content/10.1101/2025.07.22.666027v3
Release Time:2026-03-18
